Cell No. : Cell Name
RCB4675 : NMuMG/Fucci(CA)2.1
update : 2025/02/26
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| Comment | Important notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator. |
| Comment from the depositor | A subline of the NMuMG cell line expressing Fucci(CA)2.1, a cell cycle marker. Cell line name : NMuMG/Fucci(CA)2.1 #2. |
| Terms and conditions | 1)The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies). 2) The RECIPIENT should obtain written approval from the UCSD using the [mCherry FORM]. 3) The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR. 4) When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. in preparation |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
MIYAWAKI, Atsushi
|
| Originator |
MIYAWAKI, Atsushi
|
| Year of deposit |
2016
|
| Original cell |
NMuMG
|
| Cloning (depositor) |
Yes
|
| Animal |
_mouse
< Mammals
|
| Strain name |
NAMRU
|
| Gender |
Female
|
| Tissue |
mammary gland
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
FucciCA
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
|
| Culture medium |
DMEM(high), 10 % FBS
|
DMEM (high glucose) + 10% FBS
|
| Antibiotics |
Penicillin, Streptomycin
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 10-20 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Reference information |
Reference |
1
|
| User's Publication |
0
|
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