Back Back
Cell No. : Cell Name
RCB4675 : NMuMG/Fucci(CA)2.1  update : 2024/01/25
CommentImportant notice regarding nomenclature of Fucci. Research tools : Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator.
Comment from the depositorA subline of the NMuMG cell line expressing Fucci(CA)2.1, a cell cycle marker. Cell line name : NMuMG/Fucci(CA)2.1 #2.
Terms and conditions1)The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKENBRC. 2) ARECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3) In publishing the research results related to the BIOLOGICAL RESOURCE, a citation of the following literature is necessary. (in preparation) 4)The RECIPIENT agrees to acknowledge the DEPOSITER in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication tot he DEPOSITER.
approver's address
RIKEN Center for Brain Science
Laboratory for Cell Function Dynamics
Hirosawa 2-1, Wako, Saitama 351-0198 Japan
Fax. +81-48-467-5924
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor MIYAWAKI, Atsushi
Originator MIYAWAKI, Atsushi
Year of deposit 2016
Original cell NMuMG
Cloning (depositor) Yes
Animal _mouse < Mammals
Strain name NAMRU
Gender Female
Tissue mammary gland
Classification transformed
Recombinant recombinant
Exogene FucciCA
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM(high), 10 % FBS DMEM (high glucose) + 10% FBS
Antibiotics Penicillin, Streptomycin Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 10-20 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Reference information Reference 1
User's Publication 0

To topTop
10584  Sakaue-Sawano A, Yo M, Komatsu N, Hiratsuka T, Kogure T, Hoshida T, Goshima N, Matsuda M, Miyoshi H, Miyawaki A.  Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle.  Mol. Cell  2017  68(3):626-640  PubMed ID: 29107535   DOI: 10.1016/j.molcel.2017.10.001

To topTop
User's Publication

Back Back Return Top Page