Cell No. : Cell Name
RCB4674 : ICH-ERMS-1
update : 2024/01/17
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| Comment | |
| Comment from the depositor | The cell line has been established from a biopsied sample with an aplastic embryonal rhabdomyosarcoma originating in the pelvis of 5-year-old female. The biopsy revealed TP53 c.733G>A; however, the autopsy and established cell line showed TP53 c.743G>T. |
| Terms and conditions | 1) There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Kato K,et al. Pediatr Int . 2021 2021 63(7):782-789) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
KATO, Keisuke
|
| Originator |
KATO, Keisuke
|
| Year of deposit |
2016
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Asian
|
| Gender |
Female
|
| Age at sampling |
5 years
|
| Tissue |
pelvic tumor
|
| Primary focus |
retroperitoneum
|
| Disease name |
rhabdomyosarcoma
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
RPMI1640 + 10% FBS
|
RPMI1640 + 10% FBS
|
| Antibiotics |
PC + SM
|
Free
|
|
Passage method |
EDTA + Trypsin
|
0.25% Trypsin
|
Culture information
|
Passage cell No |
1×10 5-10 6 cells / 25mL flask
|
|
| Passage ratio |
|
1 : 5
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| SC frequency |
|
Subculture or Medium Renewal : once/3-4 days
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
0
|
| Reference |
|
15873
Kato K, Yoshimi A, Noda A, Otani H, Hojo H, Tanaka M, Tanaka Y, Ito Y, Nishimura R, Takita J, Yanai T, Koike K, Tsuchida M.
Distinct clonal evolution in a case with anaplastic embryonal rhabdomyosarcoma
Pediatr Int . 2021
2021
63(7):782-789
PubMed ID: 33021000
DOI: 10.1111/ped.14499
|
|
15872
Nishimura R, Takita J, Sato-Otsubo A, Kato M, Koh K, Hanada R, Tanaka Y, Kato K, Maeda D, Fukayama M, Sanada M, Hayashi Y, Ogawa S.
Characterization of genetic lesions in rhabdomyosarcoma using a high-density single nucleotide polymorphism array
Cancer Sci
2013
104(7):856-64
PubMed ID: 23578105
DOI: 10.1111/cas.12173
|
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