Cell No. : Cell Name
RCB4670 : KN7
update : 2024/11/11
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| Comment | |
| Comment from the depositor | KN7 is an anti-idiyotypic antibody for TCRVα5Vβ11 on CD4 T celIs wihich recognize Peptide-25 of A285B from Mycobacterium tuberculosis. |
| Terms and conditions | 1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Int Immunol. 2004;16(12):1691-9) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
NAGAI, Yoshinori
|
| Originator |
TAKATSU, Kiyoshi
|
| Year of deposit |
2016
|
| Cloning (depositor) |
Yes
|
| _Method (depositor) |
limiting dilution
|
| Animal |
_mouse x rat
< Hybrid
|
| Tissue |
hybrid cells
|
| Recombinant |
non-recombinant
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_A7DM
|
|
htype |
B
|
hybridoma
|
Fusion_cell_1 name |
Lymph node cells
|
| Fusion_cell_1 strain |
rat
|
| Fusion_cell_1 gender |
Female
|
| Fusion_cell_2 name |
SP2/0
|
| Fusion_cell_2 strain |
mouse
|
|
Fusion method |
PEG1500, HAT selection
|
|
Antibody name |
TCRVα5Vβ11
|
| Subclass |
IgG1
|
| Immunogen |
TG40-BP1
|
|
Antigen_character |
Peptide-25 of A285B from Mycobacterium tuberculosis
|
|
Antigen_positive |
CD4 T cell
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Suspension cells
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
RPMI1640(Gibco), 10%FCS(HyClone), 5x10 -5M 2-mercaptoethanol
|
RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
|
| Antibiotics |
Penicillin and Streptomycin
|
Free
|
|
Passage method |
|
dilution
|
| Designated culture dish |
EasYFlask (NUNC)
|
|
Culture information
|
Passage cell No |
2x10 4 cells/plate
|
|
| Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
FCS+10%DMSO
|
90% FBS + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgG1
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
1
|
| User's Publication |
0
|
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