Cell No. : Cell Name
RCB4669 : id5krm3
update : 2024/11/11
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| Comment | |
| Comment from the depositor | id5krm3 cell line has established from d5P cell line. Moreover this cell line has highly metastatic activity to lung by intracapsular transplantation. |
| Terms and conditions | Basically, there is no restriction regarding academic use. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
YAMANO, Shotaro
|
| Originator |
YAMANO, Shotaro
|
| Year of deposit |
2016
|
| Another name |
id5krm#3
|
| Animal |
_rat
< Mammals
|
| Strain name |
Wistar, Crlj:WI
|
| Gender |
Male
|
| Age at sampling |
25 weeks
|
| Tissue |
kidney
|
| Tumor |
renal cell carcinoma
|
| Classification |
cancer
|
| Recombinant |
non-recombinant
|
| Exogene |
EGFP, Luc2
|
| Recombinant virus |
No
|
|
Lifespan |
infinite
|
| Morphology |
other
(colony-formaiton)
|
| Cellosaurus(Expasy) |
CVCL_A6ZL
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM(low glucose), 10%FBS
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
1%P/S
|
Free
|
|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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Culture information
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Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture or Medium Renewal : once/2 days
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
CELLBANKER
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Reference information |
Reference |
0
|
| User's Publication |
0
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