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Cell No. : Cell Name
RCB4669 : id5krm3  update : 2024/01/17
Comment from the depositorid5krm3 cell line has established from d5P cell line. Moreover this cell line has highly metastatic activity to lung by intracapsular transplantation.
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor YAMANO, Shotaro
Originator YAMANO, Shotaro
Year of deposit 2016
Another name id5krm#3
Animal _rat < Mammals
Strain name Wistar, Crlj:WI
Gender Male
Age at sampling 25 weeks
Tissue kidney
Tumor renal cell carcinoma
Classification cancer
Recombinant non-recombinant
Exogene EGFP, Luc2
Recombinant virus No
Lifespan infinite
Morphology other (colony-formaiton)
Cellosaurus(Expasy) CVCL_A6ZL
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM(low glucose), 10%FBS DMEM (low glucose) + 10% FBS
Antibiotics 1%P/S Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 4 split
SC frequency Subculture or Medium Renewal : once/2 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium CELLBANKER Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Reference information Reference 0
User's Publication 0

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