Cell No. : Cell Name
RCB4667 : id5P
update : 2024/01/17
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| Comment | |
| Comment from the depositor | Renal cell carcinoma cell line d5P has established from renal tubule cells of Crli:WI rat. Moreover this cell line is able to do transplantation for wild type CrlJ:WI rat. |
| Terms and conditions | Basically, there is no restriction regarding academic use. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
YAMANO, Shotaro
|
| Originator |
YAMANO, Shotaro
|
| Year of deposit |
2016
|
| Another name |
rRCCd5, d5
|
| Animal |
_rat
< Mammals
|
| Strain name |
Wistar, Crlj:WI
|
| Gender |
Male
|
| Age at sampling |
25 weeks
|
| Tissue |
kidney
|
| Tumor |
renal cell carcinoma
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Exogene |
EGFP, Luc2
|
| Recombinant virus |
No
|
|
Morphology |
other
(colony-formaiton)
|
| Cellosaurus(Expasy) |
CVCL_A6ZK
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM(low glucose), 10%FBS
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
1%P/S
|
Free
|
|
Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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Culture information
|
Passage ratio |
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1 : 4-8 split
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| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
CELLBANKER
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Reference information |
Reference |
0
|
| User's Publication |
0
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