Cell No. : Cell Name
RCB4664 : d5P
update : 2024/11/11
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| Comment | |
| Comment from the depositor | Renal cell carcinoma cell line d5P has established from renal tubule cells of Crlj:WI rat. Moreover this cell line is able to do transplantation for wild type Crlj:WI rat. |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
YAMANO, Shotaro
|
| Originator |
YAMANO, Shotaro
|
| Year of deposit |
2016
|
| Another name |
rRCCd5, d5
|
| Animal |
_rat
< Mammals
|
| Strain name |
Wistar, Crlj:WI
|
| Gender |
Male
|
| Age at sampling |
25 weeks
|
| Tissue |
kidney
|
| Tumor |
renal cell carcinoma
|
| Classification |
cancer
|
| Recombinant |
non-recombinant
|
|
Lifespan |
infinite
|
| Morphology |
other
(colony-formation)
|
| Cellosaurus(Expasy) |
CVCL_A6ZH
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM(lowg lucose), 10%FBS
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
1%P/S
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Free
|
|
Passage method |
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0.25% Trypsin+ (0.02% EDTA or 0.04% EDTA)
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Culture information
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Passage ratio |
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1 : 4-8 split
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| SC frequency |
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Subculture : 2 times/week, Medium change : every other day
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| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
CELLBANKER
|
Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
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| Reference information |
Reference |
0
|
| User's Publication |
0
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