Cell No. : Cell Name RCB4662 : HCT116 CMV-OsTIR1  update : 2024/08/14
Comment			cf.： Kanemaki's Auxin Inducible Degron (AID) System
Comment from the depositor			This is an HCT116 cell line in which CMV-OsTIR1 (pMK232, PURO) was introduced at the both alleles of the AAVS1 locus by using CRISPR/Cas.This parental cell line consitutively express OsTIR1 and can be used for generation of AID mutants.
Terms and conditions			In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Rep 2016;15:210-218) designated by the DEPOSITOR is required.
Remarks			CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology.
Order Form			Order Form(C-0005.pdf)   MTA(C-0065.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Kanemaki, Masato
		Originator	Kanemaki, Masato
		Year of deposit	2016
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Tissue	colon
		Classification	cancer
		Recombinant	recombinant
		Exogene	CMV-OsTIR1, pMK232, PURO
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_RJ11
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		McCoy's 5A (with Glutamine) + 2mM L-Glutamine + 10% FBS
		Antibiotics		free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	3
		User's Publication	2

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Reference

4031  Tungadi EA, Ito A, Kiyomitsu T, Goshima G.  Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2  J Cell Sci  2017  130(21):3676-3684  PubMed ID: 28883092   DOI: 10.1242/jcs.203703

4030  Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT.  Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors  Cell Rep  2016  15:210-218  PubMed ID: 27052166   DOI: 10.1016/j.celrep.2016.03.001

5491  Brattain MG, Fine WD, Khaled FM, Thompson J, Brattain DE.  Heterogeneity of malignant cells from a human colonic carcinoma.  Cancer Res  1981  41(5):1751-6  PubMed ID: 7214343

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User's Publication

21860  Duan H, Zhang S, Zarai Y, Öllinger R, Wu Y, Sun L, Hu C, He Y, Tian G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA.  eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content  EMBO J .  2023  42(12):e112362  PubMed ID: 37155573   DOI: 10.15252/embj.2022112362

21861  Kikuchi A, Onoda H, Yamaguchi K, Kori S, Matsuzawa S, Chiba Y, Tanimoto S, Yoshimi S, Sato H, Yamagata A, Shirouzu M, Adachi N, Sharif J, Koseki H, Nishiyama A, Nakanishi M, Defossez PA, Arita K.  Structural basis for activation of DNMT1  Nat Commun  2022  13(1):7130  PubMed ID: 36414620   DOI: 10.1038/s41467-022-34779-4