Hepa/8F5 derived from mouse hepatoma Hepa1-6 [RCB1638] cells was established by introducing doxycycline (Dox)-inducible eight liver-enriched transcription factor (LETF) genes. Upon overexpression of LETF genes by Dox addition, cell proliferation ceases and the cells change the morphology expressing high liver functions.
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6417
Hiroyuki Kitano . Yuki Nagae . Yoshinori Kawabe . Akira Ito . Masamichi Kamihira
Development of a genetically modified hepatoma cell line with heat-inducible high liver functionCytotechnology
2021
24 February
PubMed ID: 34149171
DOI: 10.1007/s10616-021-00457-4
7602
Fujii Y, Higashi K, Mizumoto H, Kamihira M, Kajiwara T.
A bioartificial liver device based on three-dimensional culture of genetically engineered hepatoma cells using hollow fibers.Cytotechnology
2020
72(2):227-237
PubMed ID: 32016712
DOI: 10.1007/s10616-020-00372-0
4517
Yamamoto H, Tonello JM, Sambuichi T, Kawabe Y, Ito A, Kamihira M.
Characterization of genetically engineered mouse hepatoma cells with inducible liver functions by overexpression of liver-enriched transcription factors.J Biosci Bioeng.
2018
125(1):131-139
PubMed ID: 28847578
DOI: 10.1016/j.jbiosc.2017.07.011
12242
Joyita Sarkar, Jyoti Kumari, Jane M Tonello, Masamichi Kamihira, Ashok Kumar
Enhanced Hepatic Functions of Genetically Modified Mouse Hepatoma Cells by Spheroid Culture for Drug Toxicity ScreeningBiotechnol J
2017
12(10)
PubMed ID: 28834334
DOI: 10.1002/biot.201700274
12895
Jane Marie Tonello, Saori Kawashima, Kazuki Sato, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira
Three-dimensional culture of a genetically modified hepatoma cell line using macroporous gelatin beadsCytotechnology
2017
69(6):925-931
PubMed ID: 28689279
DOI: 10.1007/s10616-017-0117-0
7580
Hideaki Yamamoto, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira
Enhanced liver functions in mouse hepatoma cells by induced overexpression of liver-enriched transcription factorBiochemical Engineering Journal
2012
60:67-73
DOI: 10.1016/j.bej.2011.10.004