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Cell No. : Cell Name
RCB4661 : Hepa/8F5  update : 2024/01/17
Comment
Comment from the depositorHepa/8F5 derived from mouse hepatoma Hepa1-6 [RCB1638] cells was established by introducing doxycycline (Dox)-inducible eight liver-enriched transcription factor (LETF) genes. Upon overexpression of LETF genes by Dox addition, cell proliferation ceases and the cells change the morphology expressing high liver functions.
Terms and conditions1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Yamamoto et al. Biochemical Engineering Journal 2012 60, 15:67-73) designated by the DEPOSITOR is requested. 2) The Modification of the BIOLOGICAL RESOURSE shall be not created without prior written consent of the DEPOSITOR. 3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURSE.
Remarks
approver's address
×
English
Address
Dept. Chem. Eng. Facultry of Eng. Kyushu Univ.
744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan
Dr.Kamihira Masamichi
Fax. +81-92-802-2753
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor KAMIHIRA, Masamichi
Originator KAMIHIRA, Masamichi
Year of deposit 2016
Another name Hepa8F
Original cell Hepa 1-6 (RCB1638)
Cloning (depositor) Yes
_Date (depositor) 2013/08
Animal _mouse < Mammals
Strain name C57L
Genus Mus
Species musculus
Tissue liver
Disease name hepatopma
Classification cancer
Exogene HNF1α, HNF1β, HNF3β, HNF4α, HNF6, C/EBPα, C/EBPβ, C/EBPγ
Lifespan infinite
Morphology epithelial-like
Differentiation Liver functions in Hepa/8F5 cell : Dox (0.1 mg/mL)
Cellosaurus(Expasy) CVCL_RI52
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM(high glucose) + 10% FBS DMEM (high glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4-8 split 1 : 4-8 split
SC frequency Subculture : 2-3 times/week Subculture : 2-3 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Reference information Reference 6
User's Publication 0


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Reference
6417  Hiroyuki Kitano . Yuki Nagae . Yoshinori Kawabe . Akira Ito . Masamichi Kamihira  Development of a genetically modified hepatoma cell line with heat-inducible high liver function  Cytotechnology  2021  24 February  PubMed ID: 34149171   DOI: 10.1007/s10616-021-00457-4
7602  Fujii Y, Higashi K, Mizumoto H, Kamihira M, Kajiwara T.  A bioartificial liver device based on three-dimensional culture of genetically engineered hepatoma cells using hollow fibers.  Cytotechnology  2020  72(2):227-237  PubMed ID: 32016712   DOI: 10.1007/s10616-020-00372-0
4517  Yamamoto H, Tonello JM, Sambuichi T, Kawabe Y, Ito A, Kamihira M.  Characterization of genetically engineered mouse hepatoma cells with inducible liver functions by overexpression of liver-enriched transcription factors.  J Biosci Bioeng.  2018  125(1):131-139  PubMed ID: 28847578   DOI: 10.1016/j.jbiosc.2017.07.011
12242  Joyita Sarkar, Jyoti Kumari, Jane M Tonello, Masamichi Kamihira, Ashok Kumar  Enhanced Hepatic Functions of Genetically Modified Mouse Hepatoma Cells by Spheroid Culture for Drug Toxicity Screening  Biotechnol J  2017  12(10)  PubMed ID: 28834334   DOI: 10.1002/biot.201700274
12895  Jane Marie Tonello, Saori Kawashima, Kazuki Sato, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira  Three-dimensional culture of a genetically modified hepatoma cell line using macroporous gelatin beads  Cytotechnology  2017  69(6):925-931  PubMed ID: 28689279   DOI: 10.1007/s10616-017-0117-0
7580  Hideaki Yamamoto, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira  Enhanced liver functions in mouse hepatoma cells by induced overexpression of liver-enriched transcription factor  Biochemical Engineering Journal  2012  60:67-73    DOI: 10.1016/j.bej.2011.10.004

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