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Cell No. : Cell Name
RCB4638 : M81 update : 2024/11/11 |
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| Comment | ||||
| Comment from the depositor | Anti-human C1s monoclonal antibody producing cell. | |||
| Terms and conditions | 1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | IPR | Hokkaido University (Japan) | ||
| Depositor | MATSUMOTO, Misako | |||
| Originator | MATSUMOTO, Misako | |||
| Year of deposit | 2015 | |||
| Animal | _mouse x mouse < Hybrid | |||
| Tissue | hybridoma | |||
| Classification | hybrid cells | Lifespan | infinite | |
| Morphology | lymphocyte-like | hybridoma | Fusion_cell_1 name | spleen |
| Fusion_cell_1 strain | mouse (C57BL/6 x Balb/c F1) | |||
| Fusion_cell_1 gender | Female | |||
| Fusion_cell_2 name | NS-1 | |||
| Fusion_cell_2 strain | mouse, BALB/c | Fusion method | PEG, HAT selection | Antibody name | M81 |
| Subclass | IgG1(Kappa) | |||
| Immunogen | Activated human C1s purified protein | Antigen_name | Inactive and active forms of subcomponent C1s of the first component of human complement C1. | |
| Antigen_m.w. | 83kDa | |||
| Antigen_character | Soluble glycoprotein | |||
| Antigen_epitope | C4 binding site on the H chain | Antigen_positive | serum | |
| Medium | Medium List | Culture type | Suspension cells | Suspension cells |
| Culture method | 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | PRMI1640 (Gibco) / 10% FCS | RPMI1640 + 10% FBS | ||
| Antibiotics | 50 U/ml penicillin, 50 μg/ml streptomaycin | Free | Passage method | dilution | Culture information | Passage cell No | 0.5-1x10 5 cells/ml |
| Passage ratio | 1 : 2-5 split | |||
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | 37 ℃ | ||
| CO2 concentration | 5 % | 5 % | ||
| Freeze medium | Bambanker | CELLBANKER 1 | ||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Antibody subclass | IgG1(Kappa) | |||
| Reference information | Reference | 2 | ||
| User's Publication | 0 | |||
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