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Cell No. : Cell Name
RCB4635 : #16-15 update : 2024/01/17 |
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| Comment | Hybridoma cell line producing anti-human BEND3 antibody (rat IgM). | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) The BIOLOGICAL RESOURSE should not used for any commercial purposes. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Immun Inflamm Dis. 2014;2:35-43) . | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Shimizu, Jun | ||
| Originator | Shimizu, Jun | |||
| Year of deposit | 2015 | |||
| Animal | _mouse x rat < Hybrid | |||
| Tissue | hybridoma | |||
| Classification | hybrid cells | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_KA96 | hybridoma | Fusion_cell_1 name | spleen cells |
| Fusion_cell_1 strain | rat, Wistar | |||
| Fusion_cell_2 name | P3-X63-Ag8.653 | |||
| Fusion_cell_2 strain | mouse | Fusion method | PEG | Antibody name | #16-15 |
| Subclass | rat IgM | |||
| Immunogen | T cells in human PBMCs | Antigen_name | human BEND3 | |
| Medium | Medium List | Culture type | Suspension cells | |
| Culture medium | DMEM (low glucose) + 10% FBS | |||
| Culture method | ||||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | 1 : 10 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
| Antibody subclass | rat IgM | |||
| Images | ||||
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| Reference information | Reference | 1 | ||
| User's Publication | 0 | |||
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