An immortalized mouse Kupffer cell line established from C57BL/6 strain. The Kupffer cells were proliferated in the mixed primary culture of C57BL/6 adult mouse liver cells, then infected with a replication-deficient retroviral vector containing the human c-myc oncogene and neomycin resistance gene, isolated and selected with G418 (cloned by a limited dilution).
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1) A permission of the approver is required. 2) If any intellectual Property is generated during the use of the delivered Material, the Recipient shall consult with the Administrator of the Material at the NARO. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Results in Immunology 2014;4:68-74) designated by the DEPOSITOR is required.
The National Agriculture and Food Research Organization Institute of Agrobiological Sciences, Division of Animal Science 1-2 Owashi, Tsukuba, Ibaraki 305-8634 Japan
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replication-deficient retroviral vector , human c-myc, Neomycin resistance gene, HSV-TK
Kitani H, Sakuma C, Takenouchi T, Sato M, Yoshioka M, Yamanaka N.
Establishment of c-myc-immortalized Kupffer cell line from a C57BL/6 mouse strain.
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