Cell No. : Cell Name
RCB4627 : KUP5
update : 2022/10/28
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| Comment | |
| Comment from the depositor | An immortalized mouse Kupffer cell line established from C57BL/6 strain. The Kupffer cells were proliferated in the mixed primary culture of C57BL/6 adult mouse liver cells, then infected with a replication-deficient retroviral vector containing the human c-myc oncogene and neomycin resistance gene, isolated and selected with G418 (cloned by a limited dilution). No significant production of infectious viral particles from KUP5 cells was confirmed by bioassay using SC-1 cells. |
| Terms and conditions | 1) There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Results Immunol 2014 4:68-74) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Kitani, Hiroshi
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| Originator |
Kitani, Hiroshi
|
| Year of deposit |
2015
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| Animal |
mouse
< Mammals
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| Strain name |
C57BL/6
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| Genus |
Mus
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| Species |
musculus
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| Gender |
Male
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| Age at sampling |
7 weeks
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| Tissue |
liver
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| Classification |
transformed
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| Recombinant |
recombinant
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| Exogene |
replication-deficient retroviral vector , human c-myc, Neomycin resistance gene, HSV-TK
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|
Lifespan |
infinite
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| Morphology |
other
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| Cellosaurus(Expasy) |
CVCL_6C88
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Medium and additives |
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DMEM (high glucose) + 10% FBS + 10μg/ml bovine Insulin + 250μM Monothioglycerol
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| Antibiotics |
|
Free
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|
Passage method |
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TrypLE Express
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| Designated culture dish |
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FALCON 1008
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Culture information
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Passage cell No |
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2-5 x 10^(5) cells/100 mm dish
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| Animal PCR |
|
OK
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| SSLP(mouse) |
|
OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
17
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| User's Publication |
|
20681
Pang Y, Yao Y, Yang M, Wu D, Ma Y, Zhang Y, Zhang T.
TFEB-lysosome pathway activation is associated with different cell death responses to carbon quantum dots in Kupffer cells and hepatocytes.
Part Fibre Toxicol
2022
19(1):31
PubMed ID: 35477523
DOI: 10.1186/s12989-022-00474-x
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16781
Jiulong Li, Graciel Diamante, In Sook Ahn, Darren Wijaya, Xiang Wang, Chong Hyun Chang, Sung-min Ha, Kavya Immadisetty, Huan Meng, Andre E. Nel, Xia Yang, Xia Tian
Determination of the Nanoparticle- and Cell-Specific Toxicological Mechanisms in 3d Liver Spheroids Using Scrnaseq Analysis
SSRN Electronic Journal
2022
DOI: 10.2139/ssrn.4139095
|
|
20007
Takata N, Ishii KA, Takayama H, Nagashimada M, Kamoshita K, Tanaka T, Kikuchi A, Takeshita Y, Matsumoto Y, Ota T, Yamamoto Y, Yamagoe S, Seki A, Sakai Y, Kaneko S, Takamura T.
LECT2 as a hepatokine links liver steatosis to inflammation via activating tissue macrophages in NASH.
Sci Rep
2021
11(1):555
PubMed ID: 33436955
DOI: 10.1038/s41598-020-80689-0
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20038
Arisaka Y, Yui N.
Molecular mobility of polyrotaxane-based biointerfaces alters inflammatory responses and polarization in Kupffer cell lines.
Biomater Sci
2021
PubMed ID: 33533783
DOI: 10.1039/d0bm02127j
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20260
Li J, Wang X, Chang CH, Jiang J, Liu Q, Liu X, Liao YP, Ma T, Meng H, Xia T.
Nanocellulose Length Determines the Differential Cytotoxic Effects and Inflammatory Responses in Macrophages and Hepatocytes.
Small
2021
e2102545
PubMed ID: 34363305
DOI: 10.1002/smll.202102545
|
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15889
Li J, Wang X, Mei KC, Chang CH, Jiang J, Liu X, Liu Q, Guiney LM, Hersam MC, Liao YP, Meng H, Xia T.
Lateral size of graphene oxide determines differential cellular uptake and cell death pathways in Kupffer cells, LSECs, and hepatocytes
Nano Today
2021
37:101061
PubMed ID: 34055032
DOI: 10.1016/j.nantod.2020.101061
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20469
Pang Y, Wu D, Ma Y, Cao Y, Liu Q, Tang M, Pu Y, Zhang T.
Reactive oxygen species trigger NF-κB-mediated NLRP3 inflammasome activation involvement in low-dose CdTe QDs exposure-induced hepatotoxicity.
Redox Biol
2021
47:102157
PubMed ID: 34614473
DOI: 10.1016/j.redox.2021.102157
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16178
Li J, Guiney LM, Downing JR, Wang X, Chang CH, Jiang J, Liu Q, Liu X, Mei KC, Liao YP, Ma T, Meng H, Hersam MC, Nel AE, Xia T.
Dissolution of 2D Molybdenum Disulfide Generates Differential Toxicity among Liver Cell Types Compared to Non-Toxic 2D Boron Nitride Effects
Small
2021
17(25):e2101084
PubMed ID: 34032006
DOI: 10.1002/smll.202101084
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16900
Islam R, Gao S, Islam W, Šubr V, Zhou JR, Yokomizo K, Etrych T, Maeda H, Fang J.
Unraveling the role of Intralipid in suppressing off-target delivery and augmenting the therapeutic effects of anticancer nanomedicines
Acta Biomater
2021
126:372-383
PubMed ID: 33774199
DOI: 10.1016/j.actbio.2021.03.044
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15261
Wang X, Chang CH, Jiang J, Liu X, Li J, Liu Q, Liao YP, Li L, Nel AE, Xia T.
Mechanistic Differences in Cell Death Responses to Metal-Based Engineered Nanomaterials in Kupffer Cells and Hepatocytes
Small
2020
16(21):e2000528
PubMed ID: 32337854
DOI: 10.1002/smll.202000528
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9620
Qi Liu, Xiang Wang, Xiangsheng Liu, Sanjan Kumar, Grant Gochman, Ying Ji, Yu-Pei Liao, Chong Hyun Chang, Wesley Situ, Jianqin Lu, Jinhong Jiang, Kuo-Ching Mei, Huan Meng, Tian Xia, Andre E Nel
Use of Polymeric Nanoparticle Platform Targeting the Liver To Induce Treg-Mediated Antigen-Specific Immune Tolerance in a Pulmonary Allergen Sensitization Model
ACS Nano
2019
13(4):4778-4794
PubMed ID: 30964276
DOI: 10.1021/acsnano.9b01444
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16898
Durymanov M, Permyakova A, Sene S, Guo A, Kroll C, Giménez-Marqués M, Serre C, Reineke J.
Cellular Uptake, Intracellular Trafficking, and Stability of Biocompatible Metal-Organic Framework (MOF) Particles in Kupffer Cells
Mol Pharm
2019
16(6):2315-2325
PubMed ID: 31012592
DOI: 10.1021/acs.molpharmaceut.8b01185
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10619
Hayashi K, Yamada S, Hayashi H, Sakamoto W, Yogo T.
Red blood cell-like particles with the ability to avoid lung and spleen accumulation for the treatment of liver fibrosis.
Biomaterials
2018
156:45-55
PubMed ID: 29190497
DOI: 10.1016/j.biomaterials.2017.11.031
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10835
Kim KH, Kim SH, Han DH, Jo YS, Lee YH, Lee MS.
Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice.
Sci Rep
2018
8:6789
PubMed ID: 29717162
DOI: 10.1038/s41598-018-25098-0
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11054
J Chilloux, F Brial, et al
Microbiome inhibition of IRAK-4 by trimethylamine mediates metabolic and immune benefits in high-fat-diet-induced insulin resistance
bioRxiv
2018
DOI: 10.1101/277434
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14535
Hayashi K, Tokuda A, Sakamoto W.
Hydroxyl Radical-Suppressing Mechanism and Efficiency of Melanin-Mimetic Nanoparticles
Int J Mol Sci
2018
19(8):2309
PubMed ID: 30087240
DOI: 10.3390/ijms19082309
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15053
Koichiro Hayashi, Takuma Maruhashi, Wataru Sakamoto, Toshinobu Yogo
Organic-Inorganic Hybrid Hollow Nanoparticles Suppress Oxidative Stress and Repair Damaged Tissues for Treatment of Hepatic Fibrosis
Advanced Functional Materials
2018
28:1706332
DOI: 10.1002/adfm.201706332
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