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Cell No. : Cell Name
RCB4627 : KUP5  update : 2021/12/24
Comment
Comment from the depositorAn immortalized mouse Kupffer cell line established from C57BL/6 strain. The Kupffer cells were proliferated in the mixed primary culture of C57BL/6 adult mouse liver cells, then infected with a replication-deficient retroviral vector containing the human c-myc oncogene and neomycin resistance gene, isolated and selected with G418 (cloned by a limited dilution). No significant production of infectious viral particles from KUP5 cells was confirmed by bioassay using SC-1 cells.
Terms and conditions1) There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Results Immunol 2014 4:68-74) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kitani, Hiroshi
Originator Kitani, Hiroshi
Year of deposit 2015
Animal _mouse < Mammals
Strain name C57BL/6
Genus Mus
Species musculus
Gender Male
Age at sampling 7 weeks
Tissue liver
Classification transformed
Recombinant recombinant
Exogene replication-deficient retroviral vector , human c-myc, Neomycin resistance gene, HSV-TK
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_6C88
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 10% FBS + 10μg/ml bovine Insulin + 250μM Monothioglycerol
Antibiotics Free
Passage method TrypLE Express
Designated culture dish FALCON 1008
Culture information Passage cell No 2-5 x 10^(5) cells/100 mm dish
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 12


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Reference
4025  Kitani H, Sakuma C, Takenouchi T, Sato M, Yoshioka M, Yamanaka N.  Establishment of c-myc-immortalized Kupffer cell line from a C57BL/6 mouse strain.  Results Immunol  2014  4:68-74  PubMed ID: 25379377

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User's Publication
20260  Li J, Wang X, Chang CH, Jiang J, Liu Q, Liu X, Liao YP, Ma T, Meng H, Xia T.  Nanocellulose Length Determines the Differential Cytotoxic Effects and Inflammatory Responses in Macrophages and Hepatocytes.  Small  21  e2102545  PubMed ID: 34363305
20007  Takata N, Ishii KA, Takayama H, Nagashimada M, Kamoshita K, Tanaka T, Kikuchi A, Takeshita Y, Matsumoto Y, Ota T, Yamamoto Y, Yamagoe S, Seki A, Sakai Y, Kaneko S, Takamura T.  LECT2 as a hepatokine links liver steatosis to inflammation via activating tissue macrophages in NASH.  Sci Rep  2021  11(1):555  PubMed ID: 33436955
20038  Arisaka Y, Yui N.  Molecular mobility of polyrotaxane-based biointerfaces alters inflammatory responses and polarization in Kupffer cell lines.  Biomater Sci  2021    PubMed ID: 33533783
15889  Li J, Wang X, Mei KC, Chang CH, Jiang J, Liu X, Liu Q, Guiney LM, Hersam MC, Liao YP, Meng H, Xia T.  Lateral size of graphene oxide determines differential cellular uptake and cell death pathways in Kupffer cells, LSECs, and hepatocytes  Nano Today  2021  37:101061  PubMed ID: 34055032
20469  Pang Y, Wu D, Ma Y, Cao Y, Liu Q, Tang M, Pu Y, Zhang T.  Reactive oxygen species trigger NF-κB-mediated NLRP3 inflammasome activation involvement in low-dose CdTe QDs exposure-induced hepatotoxicity.  Redox Biol  2021  47:102157  PubMed ID: 34614473
15261  Wang X, Chang CH, Jiang J, Liu X, Li J, Liu Q, Liao YP, Li L, Nel AE, Xia T.  Mechanistic Differences in Cell Death Responses to Metal-Based Engineered Nanomaterials in Kupffer Cells and Hepatocytes  Small  2020  16(21):e2000528  PubMed ID: 32337854
9620  Qi Liu, Xiang Wang, Xiangsheng Liu, Sanjan Kumar, Grant Gochman, Ying Ji, Yu-Pei Liao, Chong Hyun Chang, Wesley Situ, Jianqin Lu, Jinhong Jiang, Kuo-Ching Mei, Huan Meng, Tian Xia, Andre E Nel  Use of Polymeric Nanoparticle Platform Targeting the Liver To Induce Treg-Mediated Antigen-Specific Immune Tolerance in a Pulmonary Allergen Sensitization Model  ACS Nano  2019  13(4):4778-4794  PubMed ID: 30964276
10619  Hayashi K, Yamada S, Hayashi H, Sakamoto W, Yogo T.  Red blood cell-like particles with the ability to avoid lung and spleen accumulation for the treatment of liver fibrosis.  Biomaterials  2018  156:45-55  PubMed ID: 29190497
10835  Kim KH, Kim SH, Han DH, Jo YS, Lee YH, Lee MS.  Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice.  Sci Rep  2018  8:6789  PubMed ID: 29717162
11054  J Chilloux, F Brial, et al  Microbiome inhibition of IRAK-4 by trimethylamine mediates metabolic and immune benefits in high-fat-diet-induced insulin resistance  bioRxiv  2018   
14535  Hayashi K, Tokuda A, Sakamoto W.  Hydroxyl Radical-Suppressing Mechanism and Efficiency of Melanin-Mimetic Nanoparticles  Int J Mol Sci   2018  19(8):2309  PubMed ID: 30087240
15053  Koichiro Hayashi, Takuma Maruhashi, Wataru Sakamoto, Toshinobu Yogo  Organic-Inorganic Hybrid Hollow Nanoparticles Suppress Oxidative Stress and Repair Damaged Tissues for Treatment of Hepatic Fibrosis  Advanced Functional Materials  2018  28:1706332 



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