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Cell No. : Cell Name
RCB4624 : CHO-DR1000L-4N  update : 2024/01/17
Comment
Comment from the depositorCHO DG44 based recombinant cell line which produce human GM-CSF. Cell line was constructed via gene amplification by transfection of dhfr and GM-CSF genes.
Terms and conditions1) A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (T, Yoshikawa et al, Biotechnol Prog 2000 16(5):710-5) designated by the DEPOSITOR is required.
Remarks
approver's address
×
English
Address
Dept. of Material and Life Science Graduate School of Engineering Osaka University, Yamadaoka 2-1, Suita, Osaka 565-0872 Japan
Dr.Omasa Takeshi
Fax. +81-6-6879-7938
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor OMASA, Takeshi
Originator OMASA, Takeshi
Year of deposit 2015
Another name CHO-4N
Cloning (depositor) Yes
_Method (depositor) limiting dilution
Animal _hamster < Mammals
Strain name Chinese
Gender Female
Tissue ovary
Classification other
Recombinant recombinant
Exogene dihydrofolate, human GM-CSF
6800 base
Vector pSV2-dhfr/hGM-CSF
Recombinant virus No
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium IMDM(SIGMA I7633),10% dialyzed FBS(SIGMA 172012), 1000 nM Methotrexate (SIGMA A6770) IMDM + 10% dialyzed FBS + 1000 nM Methotrexate
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


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Reference
7566  Cao Y, Kimura S, Itoi T, Honda K, Ohtake H, Omasa T.  Construction of BAC-based physical map and analysis of chromosome rearrangement in Chinese hamster ovary cell lines.  Biotechnol Bioeng  2012  109(6):1357-67  PubMed ID: 22012683   DOI: 10.1002/bit.24347
7569  Omasa T, Cao Y, Park JY, Takagi Y, Kimura S, Yano H, Honda K, Asakawa S, Shimizu N, Ohtake H.  Bacterial artificial chromosome library for genome-wide analysis of Chinese hamster ovary cells.  Biotechnol Bioeng  2009  104(5):986-94  PubMed ID: 19575438   DOI: 10.1002/bit.22463
7565  Yoshikawa T, Nakanishi F, Ogura Y, Oi D, Omasa T, Katakura Y, Kishimoto M, Suga K.  Amplified gene location in chromosomal DNA affected recombinant protein production and stability of amplified genes.  Biotechnol Prog  2000  16(5):710-5  PubMed ID: 11027161   DOI: 10.1021/bp000114e

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