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Cell No. : Cell Name
RCB4623 : Ba/ER  update : 2024/01/17
CommentBa/ER was created by stable transfection of murine pro-B Ba/F3 cells with a murine erythropoietin receptor gene. Ba/ER proliferates dependent on erythropoietin or interleukin-3.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Immunol Methods 2000;241:159-170) designated by the DEPOSITOR is required.
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Basic information Depositor Kawahara, Masahiro
Originator Kawahara, Masahiro
Year of deposit 2015
Animal _mouse < Mammals
Tissue blood
Classification transformed
Recombinant recombinant
Exogene pME-ER (murine erythropoietin receptor gene), neo
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_6C87
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS + (1ng/ml mouse IL-3 or 3U/ml human EPO)
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
deposit info
lot info
Reference information Reference 1
User's Publication 0

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3665  Ueda H, Kawahara M, Aburatani T, Tsumoto K, Todokoro K, Suzuki E, Nishimura H, Schueler PA, Winter G, Mahoney WC, Kumagai I, Nagamun  Cell-growth control by monomeric antigen: the cell surface expression of lysozyme-specific Ig V-domains fused to truncated Epo receptor.  J Immunol Methods  2000  241(1-2):159-70  PubMed ID: 10915858   DOI: 10.1016/s0022-1759(00)00202-7

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