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Cell No. : Cell Name
RCB4623 : Ba/ER  update : 2024/01/17
CommentBa/ER was created by stable transfection of murine pro-B Ba/F3 cells with a murine erythropoietin receptor gene. Ba/ER proliferates dependent on erythropoietin or interleukin-3.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Immunol Methods 2000;241:159-170) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kawahara, Masahiro
Originator Kawahara, Masahiro
Year of deposit 2015
Animal _mouse < Mammals
Tissue blood
Classification transformed
Recombinant recombinant
Exogene pME-ER (murine erythropoietin receptor gene), neo
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_6C87
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS + (1ng/ml mouse IL-3 or 3U/ml human EPO)
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3665  Ueda H, Kawahara M, Aburatani T, Tsumoto K, Todokoro K, Suzuki E, Nishimura H, Schueler PA, Winter G, Mahoney WC, Kumagai I, Nagamun  Cell-growth control by monomeric antigen: the cell surface expression of lysozyme-specific Ig V-domains fused to truncated Epo receptor.  J Immunol Methods  2000  241(1-2):159-70  PubMed ID: 10915858   DOI: 10.1016/s0022-1759(00)00202-7

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