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Cell No. : Cell Name
RCB4594 : CALM KO MEF  update : 2023/10/16
CommentCALM deficient MEF cells. Tranformed with SV40 large T antigen gene.
Comment from the depositor
Terms and conditions1) Prior permission by the depositor is necessary. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS One 2012;7:e31854) designated by the DEPOSITOR is required.
Remarks
approver's address
×
English
Address
Nara Women's University
Department of Biological Sciences
Kita-Uoya-Nishimachi, Nara-Shi, Nara 630-8506 Japan
Dr.WATANABE Toshio
Fax. +81-742-20-3413
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Watanabe, Toshio
Originator Watanabe, Toshio
Year of deposit 2014
Animal _mouse < Mammals
Genus Mus
Species musculus
Age at sampling 14.5 days embryo
Tissue fetus, whole
Classification transformed
Recombinant recombinant
Exogene SV40 large T antigen gene, pGK-neo
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_6D16
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 6-8 split
SC frequency Subculture or Medium Renewal : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3661  Suzuki M, Tanaka H, Tanimura A, Tanabe K, Oe N, Rai S, Kon S, Fukumoto M, Takei K, Abe T, Matsumura I, Kanakura Y, Watanabe T.  The clathrin assembly protein PICALM is required for erythroid maturation and transferrin internalization in mice.  PLoS One  2012  7(2):e31854  PubMed ID: 22363754   DOI: 10.1371/journal.pone.0031854

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