Cell No. : Cell Name
RCB4592 : r/mHM-SFME-1
update : 2024/11/11
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| Comment | |
| Comment from the depositor | In 1987, D.Loo et al at D.Barnes lab established a pre-glial cell line SFME from Balb/c mouse embryo in serum free condition. C.Rawson et al established a tumorigenic but non-metastatic transformed cell line, ras/mycSFME, by introducing active human cH-ras and mouse c-myc into SFME cells in 1992. T.Nomura et al introduced pSV2neo into ras/mycSFME cells and discovered metastatic cells in syngeneic host lungs. Obtained mtastatic cells no longer showed neomycin resistancy. The r/mHM-SFME-1 cell line, an experimental tumor cell line carrying metastatic ability in immuno-competent Balb/c mouse, was established from the mtastasized lung in 1993 by cloning from soft agar colony,. |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (In Vitro Cell Dev Biol Anim.1993;29:614-616.) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Nomura, Takahiro
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| Originator |
Nomura, Takahiro
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| Year of deposit |
2014
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| Another name |
HM−１
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| Cloning (depositor) |
Yes
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| Animal |
_mouse
< Mammals
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| Strain name |
BALB/c
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| Genus |
Mus
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| Species |
musculus
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| Gender |
Unknown
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| Age at sampling |
embryo
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| Tissue |
pre-glia
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| Classification |
transformed
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| Recombinant |
recombinant
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| Exogene |
active human cH-ras, mouse c-myc
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|
Lifespan |
infinite
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| Morphology |
fibroblast-like
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| Cellosaurus(Expasy) |
CVCL_A6JU
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
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| Culture medium |
DMEM/HamF12 + 10% FBS (heat inactivated)
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DMEM/HamF12 + 10% FBS (heat inactivated)
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| Antibiotics |
Free
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Free
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Passage method |
0.2％ trypsin + 1mM EDTA /PBS
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 6-15 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
37
℃
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37
℃
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| CO2 concentration |
5
%
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Animal PCR |
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OK
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| SSLP(mouse) |
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OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
5
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| User's Publication |
0
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| Reference |
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5518
Matano S, Ryoyama K, Nakamura S, Okada G, Nomura T.
Application of the polymerase chain reaction (PCR) to quantify micro-metastasis in an experimental animal.
Cancer Lett
1995
91(1):93-9
PubMed ID: 7750100
DOI: 10.1016/0304-3835(94)03722-u
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5517
Nomura T, Matano S, Okada G, Tokuyama H, Hori I, Nakamura S, Kameyama T, Ryoyama K.
Establishment of a metastatic murine cell line carrying the human C-Ha-ras.
In Vitro Cell Dev Biol Anim
1993
29A(8):614-6
PubMed ID: 8376308
DOI: 10.1007/bf02634544
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1440
Rawson C, Shirahata S, Collodi P, Natsuno T, Barnes D.
Oncogene transformation frequency of nonsenescent SFME cells is increased by c-myc
Oncogene
1991
6(3):487-9
PubMed ID: 2011405
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1429
Shirahata S, Rawson C, Loo D, Chang YJ, Barnes D.
ras and neu oncogenes reverse serum inhibition and epidermal growth factor dependence of serum-free mouse embryo cells
J Cell Physiol
1990
144(1):69-76.
PubMed ID: 1973170
DOI: 10.1002/jcp.1041440110
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843
Loo D, Rawson C, Helmrich A, Barnes D.
Serum-free mouse embryo cells: growth responses in vitro
J Cell Physiol
1989
139(3):484-91.
PubMed ID: 2786879
DOI: 10.1002/jcp.1041390306
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