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Cell No. : Cell Name
RCB4592 : r/mHM-SFME-1  update : 2022/03/28
Comment from the depositorIn 1987, D.Loo et al at D.Barnes lab established a pre-glial cell line SFME from Balb/c mouse embryo in serum free condition. C.Rawson et al established a tumorigenic but non-metastatic transformed cell line, ras/mycSFME, by introducing active human cH-ras and mouse c-myc into SFME cells in 1992. T.Nomura et al introduced pSV2neo into ras/mycSFME cells and discovered metastatic cells in syngeneic host lungs. Obtained mtastatic cells no longer showed neomycin resistancy. The r/mHM-SFME-1 cell line, an experimental tumor cell line carrying metastatic ability in immuno-competent Balb/c mouse, was established from the mtastasized lung in 1993 by cloning from soft agar colony,.
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (In Vitro Cell Dev Biol Anim.1993;29:614-616.) designated by the DEPOSITOR is required.
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Basic information Depositor Nomura, Takahiro
Originator Nomura, Takahiro
Year of deposit 2014
Another name HM−1
Cloning (depositor) Yes
Animal mouse < Mammals
Strain name BALB/c
Genus Mus
Species musculus
Gender Unknown
Age at sampling embryo
Tissue pre-glia
Classification transformed
Recombinant recombinant
Exogene active human cH-ras, mouse c-myc
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_A6JU
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Medium and additives DMEM/HamF12 + 10% FBS (heat inactivated) DMEM/HamF12 + 10% FBS (heat inactivated)
Antibiotics Free Free
Passage method 0.2% trypsin + 1mM EDTA /PBS 0.25% Trypsin
Culture information Passage ratio 1 : 6-15 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
deposit info
lot info
Reference information Reference 2
User's Publication 0

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5518  Matano S, Ryoyama K, Nakamura S, Okada G, Nomura T.  Application of the polymerase chain reaction (PCR) to quantify micro-metastasis in an experimental animal.  Cancer Lett  1995  91(1):93-9  PubMed ID: 7750100
5517  Nomura T, Matano S, Okada G, Tokuyama H, Hori I, Nakamura S, Kameyama T, Ryoyama K.  Establishment of a metastatic murine cell line carrying the human C-Ha-ras.  In Vitro Cell Dev Biol Anim  1993  29A(8):614-6  PubMed ID: 8376308

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