Cell No. : Cell Name
RCB4592 : r/mHM-SFME-1
update : 2024/11/11
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| Comment | |
| Comment from the depositor | In 1987, D.Loo et al at D.Barnes lab established a pre-glial cell line SFME from Balb/c mouse embryo in serum free condition. C.Rawson et al established a tumorigenic but non-metastatic transformed cell line, ras/mycSFME, by introducing active human cH-ras and mouse c-myc into SFME cells in 1992. T.Nomura et al introduced pSV2neo into ras/mycSFME cells and discovered metastatic cells in syngeneic host lungs. Obtained mtastatic cells no longer showed neomycin resistancy. The r/mHM-SFME-1 cell line, an experimental tumor cell line carrying metastatic ability in immuno-competent Balb/c mouse, was established from the mtastasized lung in 1993 by cloning from soft agar colony,. |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (In Vitro Cell Dev Biol Anim.1993;29:614-616.) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Nomura, Takahiro
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| Originator |
Nomura, Takahiro
|
| Year of deposit |
2014
|
| Another name |
HM−１
|
| Cloning (depositor) |
Yes
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| Animal |
_mouse
< Mammals
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| Strain name |
BALB/c
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| Genus |
Mus
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| Species |
musculus
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| Gender |
Unknown
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| Age at sampling |
embryo
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| Tissue |
pre-glia
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| Classification |
transformed
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| Recombinant |
recombinant
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| Exogene |
active human cH-ras, mouse c-myc
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|
Lifespan |
infinite
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| Morphology |
fibroblast-like
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| Cellosaurus(Expasy) |
CVCL_A6JU
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
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| Culture medium |
DMEM/HamF12 + 10% FBS (heat inactivated)
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DMEM/HamF12 + 10% FBS (heat inactivated)
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| Antibiotics |
Free
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Free
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Passage method |
0.2％ trypsin + 1mM EDTA /PBS
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 6-15 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
37
℃
|
37
℃
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| CO2 concentration |
5
%
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Animal PCR |
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OK
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| SSLP(mouse) |
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OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
0
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