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Cell No. : Cell Name
RCB4592 : r/mHM-SFME-1  update : 2022/03/28
Comment
Comment from the depositorIn 1987, D.Loo et al at D.Barnes lab established a pre-glial cell line SFME from Balb/c mouse embryo in serum free condition. C.Rawson et al established a tumorigenic but non-metastatic transformed cell line, ras/mycSFME, by introducing active human cH-ras and mouse c-myc into SFME cells in 1992. T.Nomura et al introduced pSV2neo into ras/mycSFME cells and discovered metastatic cells in syngeneic host lungs. Obtained mtastatic cells no longer showed neomycin resistancy. The r/mHM-SFME-1 cell line, an experimental tumor cell line carrying metastatic ability in immuno-competent Balb/c mouse, was established from the mtastasized lung in 1993 by cloning from soft agar colony,.
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (In Vitro Cell Dev Biol Anim.1993;29:614-616.) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nomura, Takahiro
Originator Nomura, Takahiro
Year of deposit 2014
Another name HM−1
Cloning (depositor) Yes
Animal mouse < Mammals
Strain name BALB/c
Genus Mus
Species musculus
Gender Unknown
Age at sampling embryo
Tissue pre-glia
Classification transformed
Recombinant recombinant
Exogene active human cH-ras, mouse c-myc
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_A6JU
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Medium and additives DMEM/HamF12 + 10% FBS (heat inactivated) DMEM/HamF12 + 10% FBS (heat inactivated)
Antibiotics Free Free
Passage method 0.2% trypsin + 1mM EDTA /PBS 0.25% Trypsin
Culture information Passage ratio 1 : 6-15 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
5518  Matano S, Ryoyama K, Nakamura S, Okada G, Nomura T.  Application of the polymerase chain reaction (PCR) to quantify micro-metastasis in an experimental animal.  Cancer Lett  1995  91(1):93-9  PubMed ID: 7750100
5517  Nomura T, Matano S, Okada G, Tokuyama H, Hori I, Nakamura S, Kameyama T, Ryoyama K.  Establishment of a metastatic murine cell line carrying the human C-Ha-ras.  In Vitro Cell Dev Biol Anim  1993  29A(8):614-6  PubMed ID: 8376308

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