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Cell No. : Cell Name
RCB4591 : Siglec-14/THP-1 update : 2024/11/11 |
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| Comment | ||||
| Comment from the depositor | The cell line was generated by retroviral transduction of human Siglec-14 cDNA into the THP-1 cell line (JCRB0112). Basic properties are similar to those of THP-1. | |||
| Terms and conditions | 1) There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the AIST. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Glycobiology 2009 19(8):841-6) designated by the DEPOSITOR is requested. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | ANGATA, Takashi | ||
| Originator | ANGATA, Takashi | |||
| Year of deposit | 2013 | |||
| Original cell | THP-1 (JCRB0112) | |||
| Animal | _human < Mammals | |||
| Genus | Homo | |||
| Species | sapiens | |||
| Race | Japanese | |||
| Gender | Male | |||
| Tissue | peripheral blood | |||
| Disease name | acute monocytic leukemia | |||
| Classification | cancer | |||
| Recombinant | recombinant | |||
| Exogene | Siglec-14cDNA | |||
| 1200 base | ||||
| Vector | pMSCV-IRES-EGFP | |||
| Recombinant virus | No | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Differentiation | The cell line can be differentiated to macrophage-like cells by TPA (12-O-Tetradecanoylphorbol-13-acetate; i.e., phorbol acetate). (Siglec-14/THP-1 maintains the properties of the parental cell line THP-1, which can be differentiated to macrophage-like cells by TPA.) | |||
| Surface antigen (depositor) | Constitutively express human Siglec-14, a glycan-recognition protein. | |||
| Medium | Medium List | Culture type | Suspension cells | Suspension cells |
| Culture method | 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | RPMI1640 + 10%FBS | RPMI1640 + 10% FBS | ||
| Antibiotics | penicillin + streptomycin | Free | Passage method | dilution | dilution | Culture information | Passage cell No | 1x10 5 cells/ml |
| Passage ratio | 1 : 10 split | |||
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | 37 ℃ | ||
| CO2 concentration | 5 % | 5 % | ||
| Freeze medium | Cell Banker | Medium + 10% DMSO | ||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| Virus (HIV) | Undetected | |||
| Virus (HTLV-1) | (-) | |||
| Virus (EBV) | (-) | |||
| Saturation density | 1x10 6 cells/ml | |||
| Doubling time | 24-30 hr | |||
| STR(human) | OK | |||
| Reference information | Reference | 3 | ||
| User's Publication | 0 | |||
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