Cell No. : Cell Name
RCB4582 : SEAP/HeLa
update : 2024/02/28
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| Comment | |
| Comment from the depositor | The cell line expresses human secretory alkaline phosphatase. By collecting their culture media, you can measure secretory actlvlty by sensitive enzymatic assay, using Phospha-Light. |
| Terms and conditions | 1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (PLoS One 2013 8(1):e54382) designated by the DEPOSITOR is requested. 2) Non-commercial research use only. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
SATOH, Ayano
|
| Originator |
SATOH, Ayano
|
| Year of deposit |
2013
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
31 years
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Exogene |
Homo sapiens alkaline phosphatase, placental (ALPP) (NM_001632.3; GI: 94721245)
|
|
〜300
base
|
| Vector |
pCMV
|
| Selection |
200~400mg/L Hygromycin B
|
| Recombinant virus |
No
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Immunological |
The cells are positive for keratin by imunoperoxidase staining.
|
| Contact inhibition |
No
|
| Tumorigenesis |
Yes
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM+10%FBS
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
| Designated culture dish |
cell culture dish / flask
|
|
Culture information
|
Passage ratio |
subcultivation ratio of 1:2 to 1:6 is recommended.
|
1 : 4-8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Chromosome mode |
Modal number = 82; range = 70 to 164
|
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
18868
Gan W, Zhang C, Siu KY, Satoh A, Tanner JA, Yu S.
ULK1 phosphorylates Sec23A and mediates autophagy-induced inhibition of ER-to-Golgi traffic
BMC Cell Biol
2017
18(1):22
PubMed ID: 28486929
DOI: 10.1186/s12860-017-0138-8
|
|
18867
Koreishi M, Gniadek TJ, Yu S, Masuda J, Honjo Y, Satoh A.
The golgin tether giantin regulates the secretory pathway by controlling stack organization within Golgi apparatus
PLoS One
2013
8(3):e59821
PubMed ID: 23555793
DOI: 10.1371/journal.pone.0059821
|
|
7564
Koreishi M, Yu S, Oda M, Honjo Y, Satoh A.
CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking.
PLoS One
2013
8(1):e54382
PubMed ID: 23349870
DOI: 10.1371/journal.pone.0054382
|
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