The cells are fibroblasts prepared from embryos of the Ngly1 Engase-double knockout mice (congenic (>96%) to C57BL/6J mice). They were immortalized by transfection with a plasmid encoding the SV40 large T antigen.
細胞特性(寄託者記述:日)
当該細胞は、Ngly1 Engase ダブルノックアウトマウスの胎仔から調製した線維芽細胞であり(C57BL/6J バックグラウンド(>96%))、SV40 由来 large T 抗原で 不死化したものである。
使用条件(英)
A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
18864
Li ST, Hirayama H, Huang C, Matsuda T, Oka R, Yamasaki T, Kohda D, Suzuki T.
Hydrolytic activity of yeast oligosaccharyltransferase is enhanced when misfolded proteins accumulate in the endoplasmic reticulumFEBS J
2023
Nov 24
PubMed ID: 37997624
DOI: 10.1111/febs.17011
17480
Hirayama H, Suzuki T.
Assay for the peptide:N-glycanase/NGLY1 and disease-specific biomarkers for diagnosing NGLY1 deficiencyJ Biochem
2022
171(2):169-176
PubMed ID: 34791337
DOI: 10.1093/jb/mvab127
17476
Huang C, Suzuki T.
The occurrence of nonglycosylated forms of N-glycoprotein upon proteasome inhibition does not confirm cytosolic deglycosylationFEBS Lett
2020
594(9):1433-1442
PubMed ID: 31951015
DOI: 10.1002/1873-3468.13734
17477
Maynard JC, Fujihira H, Dolgonos GE, Suzuki T, Burlingame AL.
Cytosolic N-GlcNAc proteins are formed by the action of endo-β-N-acetylglucosaminidaseBiochem Biophys Res Commun
2020
530(4):719-724
PubMed ID: 32782141
DOI: 10.1016/j.bbrc.2020.06.127
17478
Fujihira H, Masahara-Negishi Y, Akimoto Y, Hirayama H, Lee HC, Story BA, Mueller WF, Jakob P, Clauder-Münster S, Steinmetz LM, Radhakrishnan SK, Kawakami H, Kamada Y, Miyoshi E, Yokomizo T, Suzuki T.
Liver-specific deletion of Ngly1 causes abnormal nuclear morphology and lipid metabolism under food stressBiochim Biophys Acta Mol Basis Dis
2020
1866(3):165588
PubMed ID: 31733337
DOI: 10.1016/j.bbadis.2019.165588
18860
Fujihira H, Masahara-Negishi Y, Tamura M, Huang C, Harada Y, Wakana S, Takakura D, Kawasaki N, Taniguchi N, Kondoh G, Yamashita T, Funakoshi Y, Suzuki T.
Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase genePLoS Genet
2017
13(4):e1006696
PubMed ID: 28426790
DOI: 10.1371/journal.pgen.1006696
18858
Harada Y, Huang C, Yamaki S, Dohmae N, Suzuki T.
Non-lysosomal Degradation of Singly Phosphorylated Oligosaccharides Initiated by the Action of a Cytosolic Endo-β-N-acetylglucosaminidaseJ Biol Chem
2016
291(15):8048-58
PubMed ID: 26858256
DOI: 10.1074/jbc.M115.685313
18856
Harada Y, Masahara-Negishi Y, Suzuki T.
Cytosolic-free oligosaccharides are predominantly generated by the degradation of dolichol-linked oligosaccharides in mammalian cellsGlycobiology
2014
25(11):1196-205
PubMed ID: 26206502
DOI: 10.1093/glycob/cwv055
13936
Wang X, Inoue S, Gu J, Miyoshi E, Noda K, Li W, Mizuno-Horikawa Y, Nakano M, Asahi M, Takahashi M, Uozumi N, Ihara S, Lee SH, Ikeda Y, Yamaguchi Y, Aze Y, Tomiyama Y, Fujii J, Suzuki K, Kondo A, Shapiro SD, Lopez-Otin C, Kuwaki T, Okabe M, Honke K, Taniguchi N.
Dysregulation of TGF-beta1 receptor activation leads to abnormal lung development and emphysema-like phenotype in core fucose-deficient miceProc Natl Acad Sci U S A
2005
102(44):15791-6
PubMed ID: 16236725
DOI: 10.1073/pnas.0507375102
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