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Cell No. : Cell Name
RCB4573 : MEF(Ngly1-/-Engase-/-)  update : 2024/11/11
Comment
Comment from the depositorThe cells are fibroblasts prepared from embryos of the Ngly1 Engase-double knockout mice (congenic (>96%) to C57BL/6J mice). They were immortalized by transfection with a plasmid encoding the SV40 large T antigen.
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
RIKEN Glycometabolic Biochemistry Laboratory.
2-1 Hirosawa, Wako, Saitama 351-0198, Japan
Dr.Suzuki, Tadashi
Fax. +81-48-462-4692
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor SUZUKI, Tadashi
Originator SUZUKI, Tadashi
Year of deposit 2014
Another name YH1
Animal _mouse < Mammals
Genus Mus
Species Musculus
Gender Unknown
Age at sampling 13.5, E14.5
Classification transformed
Recombinant recombinant
Exogene SV40 large T
5015 base
Vector pcDNA3.1/Zeo(+) (PMID: 16236725)
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM(4.5g/Lglucose)#08457-55 Nacalai tesque, Fetal bovine serum#172012-500mL Sigma-Aldrich(heat inactivated) DMEM (high glucose, HEPES) + 10% FBS (heat inactivated)
Antibiotics Free
Passage method Trypsin #32778-34 Nacalai tesque 0.05% Trypsin + 0.024% EDTA
Designated culture dish BD Falcon #353003 polystyrene
Culture information Passage cell No 0.5-1.0x10 6 cells/10cm plate 0.5-1x10 6cells/100mm dish
Passage ratio 1 : 5-10 split
SC frequency once every two days Subculture : 2-3 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER 1plus Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
SSLP(mouse) OK
Reference information Reference 9
User's Publication 0


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Reference
18864  Li ST, Hirayama H, Huang C, Matsuda T, Oka R, Yamasaki T, Kohda D, Suzuki T.  Hydrolytic activity of yeast oligosaccharyltransferase is enhanced when misfolded proteins accumulate in the endoplasmic reticulum  FEBS J  2023  Nov 24  PubMed ID: 37997624   DOI: 10.1111/febs.17011
17480  Hirayama H, Suzuki T.  Assay for the peptide:N-glycanase/NGLY1 and disease-specific biomarkers for diagnosing NGLY1 deficiency  J Biochem  2022  171(2):169-176  PubMed ID: 34791337   DOI: 10.1093/jb/mvab127
17476  Huang C, Suzuki T.  The occurrence of nonglycosylated forms of N-glycoprotein upon proteasome inhibition does not confirm cytosolic deglycosylation  FEBS Lett  2020  594(9):1433-1442  PubMed ID: 31951015   DOI: 10.1002/1873-3468.13734
17477  Maynard JC, Fujihira H, Dolgonos GE, Suzuki T, Burlingame AL.  Cytosolic N-GlcNAc proteins are formed by the action of endo-β-N-acetylglucosaminidase  Biochem Biophys Res Commun  2020  530(4):719-724  PubMed ID: 32782141   DOI: 10.1016/j.bbrc.2020.06.127
17478  Fujihira H, Masahara-Negishi Y, Akimoto Y, Hirayama H, Lee HC, Story BA, Mueller WF, Jakob P, Clauder-Münster S, Steinmetz LM, Radhakrishnan SK, Kawakami H, Kamada Y, Miyoshi E, Yokomizo T, Suzuki T.  Liver-specific deletion of Ngly1 causes abnormal nuclear morphology and lipid metabolism under food stress  Biochim Biophys Acta Mol Basis Dis  2020  1866(3):165588  PubMed ID: 31733337   DOI: 10.1016/j.bbadis.2019.165588
18860  Fujihira H, Masahara-Negishi Y, Tamura M, Huang C, Harada Y, Wakana S, Takakura D, Kawasaki N, Taniguchi N, Kondoh G, Yamashita T, Funakoshi Y, Suzuki T.  Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene  PLoS Genet  2017  13(4):e1006696  PubMed ID: 28426790   DOI: 10.1371/journal.pgen.1006696
18858  Harada Y, Huang C, Yamaki S, Dohmae N, Suzuki T.  Non-lysosomal Degradation of Singly Phosphorylated Oligosaccharides Initiated by the Action of a Cytosolic Endo-β-N-acetylglucosaminidase  J Biol Chem  2016  291(15):8048-58  PubMed ID: 26858256   DOI: 10.1074/jbc.M115.685313
18856  Harada Y, Masahara-Negishi Y, Suzuki T.  Cytosolic-free oligosaccharides are predominantly generated by the degradation of dolichol-linked oligosaccharides in mammalian cells  Glycobiology  2014  25(11):1196-205  PubMed ID: 26206502   DOI: 10.1093/glycob/cwv055
13936  Wang X, Inoue S, Gu J, Miyoshi E, Noda K, Li W, Mizuno-Horikawa Y, Nakano M, Asahi M, Takahashi M, Uozumi N, Ihara S, Lee SH, Ikeda Y, Yamaguchi Y, Aze Y, Tomiyama Y, Fujii J, Suzuki K, Kondo A, Shapiro SD, Lopez-Otin C, Kuwaki T, Okabe M, Honke K, Taniguchi N.   Dysregulation of TGF-beta1 receptor activation leads to abnormal lung development and emphysema-like phenotype in core fucose-deficient mice  Proc Natl Acad Sci U S A  2005  102(44):15791-6  PubMed ID: 16236725   DOI: 10.1073/pnas.0507375102

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