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Cell No. : Cell Name
RCB4571 : MEF(Ngly1-/-)  update : 2024/11/11
Comment
Comment from the depositorThe cells are fibroblasts prepared from embryos of the Ngly1-knockout mice (congenic (>96%) to C57BL/6J mice). They were immortalized by transfection with a plasmid encoding the SV40 large T antigen.
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
RIKEN Glycometabolic Biochemistry Laboratory.
2-1 Hirosawa, Wako, Saitama 351-0198, Japan
Dr.Suzuki, Tadashi
Fax. +81-48-462-4692
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor SUZUKI, Tadashi
Originator SUZUKI, Tadashi
Year of deposit 2014
Another name 5-1
Animal _mouse < Mammals
Genus Mus
Species Musculus
Gender Unknown
Age at sampling 13.5, E14.5
Classification transformed
Recombinant recombinant
Exogene SV40 large T
5015 base
Vector pcDNA3.1/Zeo(+) (PMID: 16236725)
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM(4.5g/Lglucose)#08457-55 Nacalai tesque, Fetal bovine serum#172012-500mL Sigma-Aldrich(heat inactivated) DMEM (high glucose, HEPES) + 10% FBS (heat inactivated)
Antibiotics Free
Passage method Trypsin #32778-34 Nacalai tesque 0.05% Trypsin + 0.024% EDTA
Designated culture dish BD Falcon #353003 polystyrene
Culture information Passage cell No 0.5-1.0x10 6 cells/10cm plate 0.5-1x10 6cells/100mm dish
Passage ratio 1 : 5-10 split
SC frequency Subculture : 2-3 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER 1plus Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
SSLP(mouse) OK
Reference information Reference 10
User's Publication 0


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Reference
17480  Hirayama H, Suzuki T.  Assay for the peptide:N-glycanase/NGLY1 and disease-specific biomarkers for diagnosing NGLY1 deficiency  J Biochem  2022  171(2):169-176  PubMed ID: 34791337   DOI: 10.1093/jb/mvab127
17477  Maynard JC, Fujihira H, Dolgonos GE, Suzuki T, Burlingame AL.  Cytosolic N-GlcNAc proteins are formed by the action of endo-β-N-acetylglucosaminidase  Biochem Biophys Res Commun  2020  530(4):719-724  PubMed ID: 32782141   DOI: 10.1016/j.bbrc.2020.06.127
17478  Fujihira H, Masahara-Negishi Y, Akimoto Y, Hirayama H, Lee HC, Story BA, Mueller WF, Jakob P, Clauder-Münster S, Steinmetz LM, Radhakrishnan SK, Kawakami H, Kamada Y, Miyoshi E, Yokomizo T, Suzuki T.  Liver-specific deletion of Ngly1 causes abnormal nuclear morphology and lipid metabolism under food stress  Biochim Biophys Acta Mol Basis Dis  2020  1866(3):165588  PubMed ID: 31733337   DOI: 10.1016/j.bbadis.2019.165588
17479  Galeone A, Adams JM, Matsuda S, Presa MF, Pandey A, Han SY, Tachida Y, Hirayama H, Vaccari T, Suzuki T, Lutz CM, Affolter M, Zuberi A, Jafar-Nejad H.  Regulation of BMP4/Dpp retrotranslocation and signaling by deglycosylation  Elife . 2020 Jul 28;9:e55596  2020  9:e55596  PubMed ID: 32720893   DOI: 10.7554/eLife.55596
18861  Yang K, Huang R, Fujihira H, Suzuki T, Yan N.  N-glycanase NGLY1 regulates mitochondrial homeostasis and inflammation through NRF1  J Exp Med  2018  215(10):2600-2616  PubMed ID: 30135079   DOI: 10.1084/jem.20180783
18859  Tomlin FM, Gerling-Driessen UIM, Liu YC, Flynn RA, Vangala JR, Lentz CS, Clauder-Muenster S, Jakob P, Mueller WF, Ordoñez-Rueda D, Paulsen M, Matsui N, Foley D, Rafalko A, Suzuki T,  Inhibition of NGLY1 Inactivates the Transcription Factor Nrf1 and Potentiates Proteasome Inhibitor Cytotoxicity  ACS Cent Sci  2017  1143-1155  PubMed ID: 29202016   DOI: 10.1021/acscentsci.7b00224
18860  Fujihira H, Masahara-Negishi Y, Tamura M, Huang C, Harada Y, Wakana S, Takakura D, Kawasaki N, Taniguchi N, Kondoh G, Yamashita T, Funakoshi Y, Suzuki T.  Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene  PLoS Genet  2017  13(4):e1006696  PubMed ID: 28426790   DOI: 10.1371/journal.pgen.1006696
18857  Huang C, Harada Y, Hosomi A, Masahara-Negishi Y, Seino J, Fujihira H, Funakoshi Y, Suzuki T, Dohmae N, Suzuki T.   Endo-β-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells  Proc Natl Acad Sci U S A  2015  112(5):1398-403  PubMed ID: 25605922   DOI: 10.1073/pnas.1414593112
18856  Harada Y, Masahara-Negishi Y, Suzuki T.  Cytosolic-free oligosaccharides are predominantly generated by the degradation of dolichol-linked oligosaccharides in mammalian cells  Glycobiology  2014  25(11):1196-205  PubMed ID: 26206502   DOI: 10.1093/glycob/cwv055
13936  Wang X, Inoue S, Gu J, Miyoshi E, Noda K, Li W, Mizuno-Horikawa Y, Nakano M, Asahi M, Takahashi M, Uozumi N, Ihara S, Lee SH, Ikeda Y, Yamaguchi Y, Aze Y, Tomiyama Y, Fujii J, Suzuki K, Kondo A, Shapiro SD, Lopez-Otin C, Kuwaki T, Okabe M, Honke K, Taniguchi N.   Dysregulation of TGF-beta1 receptor activation leads to abnormal lung development and emphysema-like phenotype in core fucose-deficient mice  Proc Natl Acad Sci U S A  2005  102(44):15791-6  PubMed ID: 16236725   DOI: 10.1073/pnas.0507375102

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