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Cell No. : Cell Name
RCB4569 : TEC3-10  update : 2022/05/30
CommentCulture of Thymic Epithelial Cell Lines., Protocol (pdf:120KB)
Comment from the depositorA mouse thymic medullary epithelial cell line. It has the ability to present soluble antigen to its antigen specific helper T cells. This cell line is available to analyze the functions of cortical epithelial cells and medullary epithelial cells that control the differentiation and selection of intrathymic T cells. It is also useful for attempts such as thymus reconstruction using these cell lines, aiming at such functional recovery.
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Immunol. 1991;132:377-390., J Exp Med. 1992;175:1601-1605. and Methods Mol Biol. 2007;380:107-123.) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor KASAI, Michiyuki
Originator KASAI, Michiyuki
Year of deposit 2014
Original cell medullary TEC line (mTEC line)
Cloning (depositor) Yes
Animal mouse < Mammals
Strain name C57BL/6
Gender Unknown
Age at sampling 15 weeks
Tissue thymus
Classification normal
Recombinant non-recombinant
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_A8W9
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Medium and additives SMEM (gibco 11380) + 10%FBS + 1μg/mL Hydrocortisone + 60μM 2-ME MEM (suspension, 2mM L-Glutamine) + 10%FBS + 1μg/mL Hydrocortisone + 60μM 2-ME
Antibiotics 25mg/500mL gentamicin Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage cell No 1×10^(5) cells / 60mm dish
Passage ratio 1 : 4-8 split
SC frequency Subculture : once/3-4 days Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Feeder cells Swiss 3T3 3T3 Swiss Albino
Feeder cells No. RCB1642
Feeder treatment X-rays X-rays 5000R
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 7
User's Publication 0


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Reference
4565  Kasai M, Tanida I, Ueno T, Kominami E, Seki S, Ikeda T, Mizuochi T.  Autophagic compartments gain access to the MHC class II compartments in thymic epithelium.  J Immunol  2009  183:7278-785  PubMed ID: 19915056
4564  Kasai M, Mizuochi T.  Derivation, Culture, and Characterization of Thymic Epithelial Cell Lines  Methods Mol Biol  2007  380:107-123  PubMed ID: 17876090
4563  Kasai M, Kropshofer H, Vogt AB, Kominami E, Mizuochi T.  CLIP-derived self peptides bound to MHC class II molecules of medullary thymic epithelial cells differ from those of cortical thymic epithelial cells in their diversity, length, and C-terminal processing.  Eur J Immunol  2000  30:3542-3551  PubMed ID: 11169395
4562  Kasai M, Kominami E, Mizuochi T.  The antigen presentation pathway in medullary thymic epithelial cells, but not that in cortical thymic epithelial cells, conforms to the endocytic pathway.  Eur J Immuno  1998  28:1867-1876  PubMed ID: 9645368
4561  Kasai M, Shirasawa T, Kitamura M, Ishido K, Kominami E, Hirokawa K.  Proenzyme from of cathepsin L produced by thymic epithelial cells promotes proliferation of immature thymocytes in the presence of IL-1, IL-7, and anti-CD3 antibody.  Cell Immunol  1993  150:124-136  PubMed ID: 8343964
4560  Mizuochi T, Kasai M, Kokuho T, Kakiuchi T, Hirokawa K.  Medullary but not cortical thymic epithelial cells present soluble antigens to helper T cells.  J Exp Med.  1992  175:1601-1605  PubMed ID: 1534114
4559  Kasai M, Hirokawa K.  A novel cofactor produced by a thymic epithelial cell line: promotion of proliferation of immature thymic lymphocytes by the presence of interleukin-1 and various mitogens.  Cell Immunol  1991  132:377-390  PubMed ID: 1988159

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