Cell No. : Cell Name
RCB4568 : TEC2-3
update : 2024/11/21
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| Comment | Culture of Thymic Epithelial Cell Lines., Protocol (pdf：120KB) |
| Comment from the depositor | A mouse thymic medullary epithelial cell line. It has the ability to present soluble antigen to its antigen specific helper T cells. This cell line is available to analyze the functions of cortical epithelial cells and medullary epithelial cells that control the differentiation and selection of intrathymic T cells. It is also useful for attempts such as thymus reconstruction using these cell lines, aiming at such functional recovery. |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Immunol. 1991;132:377-390., J Exp Med. 1992;175:1601-1605. and Methods Mol Biol. 2007;380:107-123.) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
KASAI, Michiyuki
|
| Originator |
KASAI, Michiyuki
|
| Year of deposit |
2014
|
| Original cell |
medullary TEC line (mTEC line)
|
| Cloning (depositor) |
Yes
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C57BL/6
|
| Gender |
Unknown
|
| Age at sampling |
15 weeks
|
| Tissue |
thymus
|
| Classification |
normal
|
| Recombinant |
non-recombinant
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_A8W8
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
SMEM (gibco 11380) + 10%FBS + 1μg/mL Hydrocortisone + 60μM 2-ME
|
MEM (suspension, 2mM L-Glutamine) + 10%FBS + 1μg/mL Hydrocortisone + 60μM 2-ME
|
| Antibiotics |
25mg/500mL gentamicin
|
Free
|
|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
|
Culture information
|
Passage cell No |
1×10 5 cells / 60mm dish
|
|
| Passage ratio |
|
1 : 4-8 split
|
| SC frequency |
Subculture : once/3-4 days
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Feeder cells |
|
3T3 Swiss Albino
|
| Feeder cells No. |
Swiss 3T3
|
RCB1642
|
| Feeder treatment |
X-rays
|
X-rays 5000R
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Images |
deposit info | lot info |
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|
|
| Reference information |
Reference |
7
|
| User's Publication |
0
|
| Reference |
|
4565
Kasai M, Tanida I, Ueno T, Kominami E, Seki S, Ikeda T, Mizuochi T.
Autophagic compartments gain access to the MHC class II compartments in thymic epithelium.
J Immunol
2009
183:7278-785
PubMed ID: 19915056
DOI: 10.4049/jimmunol.0804087
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|
4564
Kasai M, Mizuochi T.
Derivation, Culture, and Characterization of Thymic Epithelial Cell Lines
Methods Mol Biol
2007
380:107-123
PubMed ID: 17876090
DOI: 10.1007/978-1-59745-395-0_7
|
|
4563
Kasai M, Kropshofer H, Vogt AB, Kominami E, Mizuochi T.
CLIP-derived self peptides bound to MHC class II molecules of medullary thymic epithelial cells differ from those of cortical thymic epithelial cells in their diversity, length, and C-terminal processing.
Eur J Immunol
2000
30:3542-3551
PubMed ID: 11169395
DOI: 10.1002/1521-4141(200012)30:12<3542::AID-IMMU3542>3.0.CO;2-N
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|
4562
Kasai M, Kominami E, Mizuochi T.
The antigen presentation pathway in medullary thymic epithelial cells, but not that in cortical thymic epithelial cells, conforms to the endocytic pathway.
Eur J Immuno
1998
28:1867-1876
PubMed ID: 9645368
DOI: 10.1002/(SICI)1521-4141(199806)28:06<1867::AID-IMMU1867>3.0.CO;2-K
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4561
Kasai M, Shirasawa T, Kitamura M, Ishido K, Kominami E, Hirokawa K.
Proenzyme from of cathepsin L produced by thymic epithelial cells promotes proliferation of immature thymocytes in the presence of IL-1, IL-7, and anti-CD3 antibody.
Cell Immunol
1993
150:124-136
PubMed ID: 8343964
DOI: 10.1006/cimm.1993.1184
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4560
Mizuochi T, Kasai M, Kokuho T, Kakiuchi T, Hirokawa K.
Medullary but not cortical thymic epithelial cells present soluble antigens to helper T cells.
J Exp Med.
1992
175:1601-1605
PubMed ID: 1534114
DOI: 10.1084/jem.175.6.1601
|
|
4559
Kasai M, Hirokawa K.
A novel cofactor produced by a thymic epithelial cell line: promotion of proliferation of immature thymic lymphocytes by the presence of interleukin-1 and various mitogens.
Cell Immunol
1991
132:377-390
PubMed ID: 1988159
DOI: 10.1016/0008-8749(91)90035-a
|
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