Cell No. : Cell Name
RCB4562 : HUDEP-3
update : 2024/11/11
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| Comment | Human erythroid progenitor cell line derived from umbilical cord blood cells. Expressing fetal type hemoglobin. |
| Comment from the depositor | |
| Terms and conditions | 1) BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. Regarding for-profit use and/or use by for-profit organization please contact us (cellbank.brc@riken.jp). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (PLoS One. 2013;8(3):e59890) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
IPR |
RIKEN BioResource Research Center (Japan)
|
| Depositor |
KURITA, Ryo
|
| Originator |
KURITA, Ryo
|
| Year of deposit |
2013
|
| Cloning (depositor) |
No
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Tissue |
umbilical cord blood
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
KUSABIRA-ORANGE, HPV16 E6/E7
|
| Vector |
CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_VI07
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Suspension cells
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Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
StemSpan SFEM (Stemcell technologies), EPO(3U/ml), SCF(50ng/ml), DEX(10-6M), DOX(1ug/ml)
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StemSpan SFEM + 3U/ml EPO + 50ng/ml SCF + 10 -6M Dexamethasone + 1ug/ml Doxycycline
|
| Antibiotics |
|
Free
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|
Passage method |
|
dilution
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Culture information
|
Passage ratio |
Subculture : 2 times/week
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1 : 2-4 split
|
| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
CELLBANKER 1
|
| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
5
|
| User's Publication |
3
|
| Reference |
|
17722
Soboleva S, Kurita R, Ek F, Åkerstrand H, Silvério-Alves R, Olsson R, Nakamura Y, Miharada K.
Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines
Commun Biol
2021
4(1):677
PubMed ID: 34083702
DOI: 10.1038/s42003-021-02202-1
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6752
Svetlana Soboleva , Ryo Kurita, PhD , Fredrik Ek, PhD , Rita Alves , Roger Olsson, PhD , Yukio Nakamura, MD PhD , Kenichi Miharada, PhD
Identification of Potential Chemical Compounds Able to Trigger Enucleation of Immortalized Human Erythroid Cell Lines
Blood
2019
134 (Supplement_1): 951
DOI: 10.1182/blood-2019-128427
|
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6770
Melanie Demers, PhD , Sarah Sturtevant, BSc , Kevin Guertin, PhD , Dipti Gupta , Kunal Desai, MS , Benjamin Vieira, BS , Alexandra Hicks, PhD , Ayman Ismail, PhD , Yukio Nakamura, MD PhD , Bronner Goncalves, MD PhD , Giuseppe Di Caprio, PhD , Ethan Schonbrun, PhD , Scott Hansen, BS , Martin K. Safo, PhD , David K. Wood, PhD , John M. Higgins, MD PhD , David R. Light, PhD
MetAP2 Inhibition Modifies Hemoglobin S (HbS) to Delay Polymerization and Improve Blood Flow in Sickle Cell Disease
Blood
2019
134 (Supplement_1): 2260
DOI: 10.1182/blood-2019-128727
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6761
Lee E, Sivalingam J, Lim ZR, Chia G, Shi LG, Roberts M, Loh YH, Reuveny S, Oh SK.
Review: In vitro generation of red blood cells for transfusion medicine: Progress, prospects and challenges.
Biotechnol Adv
2018
36(8):2118-2128
PubMed ID: 30273713
DOI: 10.1016/j.biotechadv.2018.09.006
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6742
Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y.
Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.
PLoS One
2013
8(3):e59890
PubMed ID: 23533656
DOI: 10.1371/journal.pone.0059890
|
| User's Publication |
|
18564
Bircher C, Bieri J, Assaraf R, Leisi R, Ros C.
A Conserved Receptor-Binding Domain in the VP1u of Primate Erythroparvoviruses Determines the Marked Tropism for Erythroid Cells
Viruses
2022
14(2):420
PubMed ID: 35216013
DOI: 10.3390/v14020420
|
|
16062
Demers M, Sturtevant S, Guertin KR, Gupta D, Desai K, Vieira BF, Li W, Hicks A, Ismail A, Gonçalves BP, Di Caprio G, Schonbrun E, Hansen S, Musayev FN, Safo MK, Wood DK, Higgins JM, Light DR.
MetAP2 inhibition modifies hemoglobin S to delay polymerization and improves blood flow in sickle cell disease
Blood Adv . 2021
2021
1388-1402
PubMed ID: 33661300
DOI: 10.1182/bloodadvances.2020003670
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4518
Vinjamur DS, Bauer DE
Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines.
Erythropoiesis
2018
275-284
PubMed ID: 29076097
DOI: 10.1007/978-1-4939-7428-3_17
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