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Cell No. : Cell Name
RCB4562 : HUDEP-3 update : 2025/01/09 |
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| Comment | Human erythroid progenitor cell line derived from umbilical cord blood cells. Expressing fetal type hemoglobin. | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. Regarding for-profit use and/or use by for-profit organization please contact us (cellbank.brc@riken.jp). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (PLoS One. 2013;8(3):e59890) designated by the DEPOSITOR is required. 3) When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004). | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | IPR | RIKEN BioResource Research Center (Japan) | ||
| Depositor | KURITA, Ryo | |||
| Originator | KURITA, Ryo | |||
| Year of deposit | 2013 | |||
| Cloning (depositor) | No | |||
| Animal | _human < Mammals | |||
| Genus | Homo | |||
| Species | sapiens | |||
| Race | Japanese | |||
| Tissue | umbilical cord blood | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | KUSABIRA-ORANGE, HPV16 E6/E7 | |||
| Vector | CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_VI07 | |||
| Medium | Medium List | Culture type | Suspension cells | Suspension cells |
| Culture method | General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | StemSpan SFEM (Stemcell technologies), EPO(3U/ml), SCF(50ng/ml), DEX(10-6M), DOX(1ug/ml) | StemSpan SFEM + 3U/ml EPO + 50ng/ml SCF + 10 -6M Dexamethasone + 1ug/ml Doxycycline | ||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | Subculture : 2 times/week | 1 : 2-4 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | 37 ℃ | ||
| CO2 concentration | 5 % | 5 % | ||
| Freeze medium | Medium + 10% DMSO | CELLBANKER 1 | ||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| Virus (HIV) | Undetected | |||
| Virus (HTLV-1) | (-) | |||
| STR(human) | OK | |||
| Reference information | Reference | 5 | ||
| User's Publication | 3 | |||
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