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Cell No. : Cell Name
RCB4561 : MOLT-17  update : 2022/01/07
CommentHuman cell line derived from T cell leukemia (T-ALL). Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to DSMZ no.: ACC 36 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 36 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor DSMZ
Year of deposit 2014
Original cell ACC 36
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 5 years
Tissue Peripheral blood lymphocytes
Classification cancer
History Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan → DSMZ(ACC 36)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2125
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS (heat inactivated)
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8-16 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) Undetected
Virus (HTLV-1) (-)
STR(human) OK
Reference information Reference 2
User's Publication 0


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Reference
5516  Drexler HG1, Minowada J.  Morphological, immunophenotypical and isoenzymatic profiles of human leukemia cells and derived T-cell lines.  Hematol Oncol  1989  7(2):115-25.  PubMed ID: 2784121   DOI: 10.1002/hon.2900070203
3544  Tighe L, Forster A, Clark DM, Boylston AW, Lavenir I, Rabbitts TH.  Unusual forms of T cell gamma mRNA in a human T cell leukemia cell line: implications for gamma gene expression.  Eur J Immunol  1987  17(12):1729-36  PubMed ID: 2961573   DOI: 10.1002/eji.1830171208

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