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Cell No. : Cell Name
RCB4560 : Ham-1  update : 2024/11/11
Comment
Comment from the depositorScreening of anticancer drug, Transplantable in immunodeficient mice, In vivo model of cholangiocarcinoma.
Terms and conditions1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Korean J Parasitol 2013 51(6):711-7) designated by the DEPOSITOR is requested. 2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR or DEVELOPER. 3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Remarks
approver's address
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English
Address
Kumamoto University, The Joint Research Center for Human Retrovirus Infection
Honjo 2-2-1, Chuo-ku, Kumamoto City, Japan 860-0811
Dr.Okada, Seiji
Fax. +81-96-373-6523
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor OKADA, Seiji
Originator Kulthida Vaeteewoottacharn
Year of deposit 2014
Cloning (depositor) No
Animal _hamster < Mammals
Strain name Syrian
Genus Mesocricetus
Species auratus
Gender Male
Age at sampling 8 months
Tissue Liver
Disease name cholangiocarcinoma
Classification cancer
Recombinant non-recombinant
Year of origin 2010
Lifespan infinite
Morphology epithelial-like
Contact inhibition No
Tumorigenesis Yes
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM 10%FCS DMEM (low glucose) + 10% FBS
Antibiotics 100u/ml PC, 100µ SM Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 6 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Mycoplasma (-)
Animal PCR OK
Doubling time 36 hr
Reference information Reference 2
User's Publication 0


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Reference
18865  Boonnate P, Vaeteewoottacharn K, Kariya R, Fujikawa S, Boonmars T, Pinlaor S, Pairojkul C, Okada S.  Mucin-producing hamster cholangiocarcinoma cell line, Ham-2, possesses the aggressive cancer phenotypes with liver and lung metastases  In Vitro Cell Dev Biol Anim  2021  57(8):825-834  PubMed ID: 34549357   DOI: 10.1007/s11626-021-00608-z
7563  Puthdee N, Vaeteewoottacharn K, Seubwai W, Wonkchalee O, Kaewkong W, Juasook A, Pinlaor S, Pairojkul C, Wongkham C, Okada S, Boonmars T, Wongkham S.  Establishment of an allo-transplantable hamster cholangiocarcinoma cell line and its application for in vivo screening of anti-cancer drugs.  Korean J Parasitol  2013  51(6):711-7  PubMed ID: 24516278   DOI: 10.3347/kjp.2013.51.6.711

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