細胞番号 : 細胞名 RCB4556 : HUDEP-1  update : 2024/01/17
細胞特性(Comment:英)			Human erythroid progenitor cell line derived from umbilical cord blood cells. Expressing fetal type hemoglobin.
細胞特性(日)			ヒト赤血球前駆細胞株。臍帯血中の赤血球前駆細胞由来。胎児型ヘモグロビン産生株。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			1) BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. Regarding for-profit use and/or use by for-profit organization please contact us (cellbank.brc@riken.jp). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (PLoS One. 2013;8(3):e59890) designated by the DEPOSITOR is required.
使用条件(日)			1) 非営利団体による学術研究に限る。営利目的の使用および/または営利団体による使用については、(cellbank.brc@riken.jp) にお問い合わせください。2) 研究成果の公表にあたって寄託者の指定する文献 (PLoS One. 2013;8(3):e59890) を引用すること。
備考(英)
備考(日)
提供申込書類(英)			Order Form(C-0005.pdf)   MTA(C-0007.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	KURITA, Ryo
		樹立者	KURITA, Ryo
		寄託日	2013
		クローニング（寄託者）	No
		動物種	_human < Mammals
		属名	Homo
		種名	sapiens
		人種	Japanese
		採取組織	umbilical cord blood
		細胞分類	transformed
		遺伝子改変	recombinant
		外来遺伝子	KUSABIRA-ORANGE, HPV16 E6/E7
		外来遺伝子（ベクター）	CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector
		細胞寿命	infinite
		細胞形態	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_VI05
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態	Suspension cells	Suspension cells
		培地	StemSpan SFEM (Stemcell technologies), EPO(3U/ml), SCF(50ng/ml), DEX(10-6M), DOX(1ug/ml)	StemSpan SFEM + 3U/ml EPO + 50ng/ml SCF + 10 -6M Dexamethasone + 1ug/ml Doxycycline
		培養方法		浮遊細胞の培養に関する一般的な注意
		抗生物質		Free
		継代方法		dilution
		継代密度		1 : 4-6 split
		継代・培地交換頻度	Subculture : 2 times/week	Subculture : 2 times/week
		培養最適温度	37 ℃	37 ℃
		二酸化炭素濃度	5 %	5 %
		凍結培地	Medium + 10% DMSO	CELLBANKER 1
		凍結方法		Slow freezing
		マイコプラズマ		(-)
		動物種PCR		OK
		ウィルス（HIV)		Undetected
		ウィルス（HTLV-1)		(-)
		個体識別検査		OK
文献情報		Reference(英)	15件
		Reference(日)	0件
		利用者成果(英)	18件
		利用者成果(日)	0件

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Reference(英)

18551  Singpant P, Tubsuwan A, Sakdee S, Ketterman AJ, Jearawiriyapaisarn N, Kurita R, Nakamura Y, Songdej D, Tangprasittipap A, Bhukhai K, Chiangjong W, Hongeng S, Saisawang C.  Recombinant Cas9 protein production in an endotoxin-free system and evaluation with editing the BCL11A gene in human cells  Protein Expr Purif  2023  210:106313  PubMed ID: 37276914   DOI: 10.1016/j.pep.2023.106313

18555  Valli R, Penzo M.  Editorial: Inherited and acquired ribosomopathies: missing puzzle pieces  Front Genet  2023  14:1194788  PubMed ID: 37124624   DOI: 10.3389/fgene.2023.1194788

18517  Mukherjee M, Rahaman M, Ray SK, Shukla PC, Dolai TK, Chakravorty N.  Revisiting fetal hemoglobin inducers in beta-hemoglobinopathies: a review of natural products, conventional and combinatorial therapies  Mol Biol Rep  2022  49(3):2359-2373  PubMed ID: 34822068   DOI: 10.1007/s11033-021-06977-8

18550  Piantanida N, La Vecchia M, Sculco M, Talmon M, Palattella G, Kurita R, Nakamura Y, Ronchi AE, Dianzani I, Ellis SR, Fresu LG, Aspesi A.  Deficiency of ribosomal protein S26, which is mutated in a subset of patients with Diamond Blackfan anemia, impairs erythroid differentiation  Front Genet  2022  13:1045236  PubMed ID: 36579335   DOI: 10.3389/fgene.2022.1045236

17722  Soboleva S, Kurita R, Ek F, Åkerstrand H, Silvério-Alves R, Olsson R, Nakamura Y, Miharada K.  Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines  Commun Biol  2021  4(1):677  PubMed ID: 34083702   DOI: 10.1038/s42003-021-02202-1

15517  Papasavva PL, Papaioannou NY, Patsali P, Kurita R, Nakamura Y, Sitarou M, Christou S, Kleanthous M, Lederer CW.  Distinct miRNA Signatures and Networks Discern Fetal from Adult Erythroid Differentiation and Primary from Immortalized Erythroid Cells  Int J Mol Sci  2021  22(7):3626.  PubMed ID: 33807258   DOI: 10.3390/ijms22073626

16066  Wessels MW, Cnossen MH, van Dijk TB, Gillemans N, Schmidt KLJ, van Lom K, Vinjamur DS, Coyne S, Kurita R, Nakamura Y, de Man SA, Pfundt R, Azmani Z, Brouwer RWW, Bauer DE, van den Hout MCGN, van IJcken WFJ, Philipsen S.  Molecular analysis of the erythroid phenotype of a patient with BCL11A haploinsufficiency  Blood Adv  2021  5(9):2339-2349  PubMed ID: 33938942   DOI: 10.1182/bloodadvances.2020003753

18553  Thalia Papayannopoulou  Control of fetal globin expression in man: new opportunities to challenge past discoveries  Exp Hematol  2020  92:43-50  PubMed ID: 32976950   DOI: 10.1016/j.exphem.2020.09.195

6752  Svetlana Soboleva , Ryo Kurita, PhD , Fredrik Ek, PhD , Rita Alves , Roger Olsson, PhD , Yukio Nakamura, MD PhD , Kenichi Miharada, PhD  Identification of Potential Chemical Compounds Able to Trigger Enucleation of Immortalized Human Erythroid Cell Lines  Blood  2019  134 (Supplement_1): 951    DOI: 10.1182/blood-2019-128427

6770  Melanie Demers, PhD , Sarah Sturtevant, BSc , Kevin Guertin, PhD , Dipti Gupta , Kunal Desai, MS , Benjamin Vieira, BS , Alexandra Hicks, PhD , Ayman Ismail, PhD , Yukio Nakamura, MD PhD , Bronner Goncalves, MD PhD , Giuseppe Di Caprio, PhD , Ethan Schonbrun, PhD , Scott Hansen, BS , Martin K. Safo, PhD , David K. Wood, PhD , John M. Higgins, MD PhD , David R. Light, PhD  MetAP2 Inhibition Modifies Hemoglobin S (HbS) to Delay Polymerization and Improve Blood Flow in Sickle Cell Disease  Blood  2019  134 (Supplement_1): 2260    DOI: 10.1182/blood-2019-128727

4974  Morrison TA, Wilcox I, Luo HY, Farrell JJ, Kurita R, Nakamura Y, Murphy GJ, Cui S, Steinberg MH, Chui DHK.  A long noncoding RNA from the HBS1L-MYB intergenic region on chr6q23 regulates human fetal hemoglobin expression.  Blood Cells Mol Dis  2018  69:1-9  PubMed ID: 29227829   DOI: 10.1016/j.bcmd.2017.11.003

6761  Lee E, Sivalingam J, Lim ZR, Chia G, Shi LG, Roberts M, Loh YH, Reuveny S, Oh SK.  Review: In vitro generation of red blood cells for transfusion medicine: Progress, prospects and challenges.  Biotechnol Adv  2018  36(8):2118-2128  PubMed ID: 30273713   DOI: 10.1016/j.biotechadv.2018.09.006

6750  Trakarnsanga K, Griffiths RE, Wilson MC, Blair A, Satchwell TJ, Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ, Frayne J.  An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.  Nat Commun  2017  8:14750  PubMed ID: 28290447   DOI: 10.1038/ncomms14750

8824  Yan Dai, PhD , Tyngwei Chen , Heba Ijaz , Elizabeth Cho , Ryo Kurita, PhD , Yukio Nakamura, MD PhD , Martin H. Steinberg, MD  SIRT1 Increases the Expression of Fetal Hemoglobin Genes in Human Adult and Cord Blood Erythroblasts  Blood  2017  130 (Supplement 1): 287    DOI: 10.1182/blood.V130.Suppl_1.287.287

6742  Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y.  Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.  PLoS One  2013  8(3):e59890  PubMed ID: 23533656   DOI: 10.1371/journal.pone.0059890

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Reference(日)

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利用者成果(英)

18552  Li D, Zhao XY, Zhou S, Hu Q, Wu F, Lee HY.  Multidimensional profiling reveals GATA1-modulated stage-specific chromatin states and functional associations during human erythropoiesis  Nucleic Acids Res  2023  51(13):6634-6653  PubMed ID: 37254808   DOI: 10.1093/nar/gkad468

21274  Chaand M, Fiore C, Johnston B, D'Ippolito A, Moon DH, Carulli JP, Shearstone JR.  Erythroid lineage chromatin accessibility maps facilitate identification and validation of NFIX as a fetal hemoglobin repressor.  Commun Biol  2023  6(1):640  PubMed ID: 37316562   DOI: 10.1038/s42003-023-05025-4

21304  Mayuranathan T, Newby GA, Feng R, Yao Y, Mayberry KD, Lazzarotto CR, Li Y, Levine RM, Nimmagadda N, Dempsey E, Kang G, Porter SN, Doerfler PA, Zhang J, Jang Y, Chen J, Bell HW, Crossley M, Bhoopalan SV, Sharma A, Tisdale JF, Pruett-Miller SM, Cheng Y, Tsai SQ, Liu DR, Weiss MJ, Yen JS.  Potent and uniform fetal hemoglobin induction via base editing.  Nat Genet  2023    PubMed ID: 37400614   DOI: 10.1038/s41588-023-01434-7

17720  Wang D, Tanaka-Yano M, Meader E, Kinney MA, Morris V, Lummertz da Rocha E, Liu N, Liu T, Zhu Q, Orkin SH, North TE, Daley GQ, Rowe RG.  Developmental maturation of the hematopoietic system controlled by a Lin28b-let-7-Cbx2 axis  Cell Rep  2022  39(1):110587  PubMed ID: 35385744   DOI: 10.1016/j.celrep.2022.110587

18435  Sun Y, Habara A, Le CQ, Nguyen N, Chen R, Murphy GJ, Chui DHK, Steinberg MH, Cui S.  Pharmacologic induction of PGC-1α stimulates fetal haemoglobin gene expression  Br J Haematol  2022  197(1):97-109  PubMed ID: 35118652   DOI: 10.1111/bjh.18042

18443  Mehta S, Buyanbat A, Kai Y, Karayel O, Goldman SR, Seruggia D, Zhang K, Fujiwara Y, Donovan KA, Zhu Q, Yang H, Nabet B, Gray NS, Mann M, Fischer ES, Adelman K, Orkin SH.  Temporal resolution of gene derepression and proteome changes upon PROTAC-mediated degradation of BCL11A protein in erythroid cells  Cell Chem Biol  2022  29(8):1273-1287  PubMed ID: 35839780   DOI: 10.1016/j.chembiol.2022.06.007

18554  Pavan AR, Lopes JR, Dos Santos JL.  The state of the art of fetal hemoglobin-inducing agents  Expert Opin Drug Discov  2022  17(11):1279-1293  PubMed ID: 36302760   DOI: 10.1080/17460441.2022.2141708

18564  Bircher C, Bieri J, Assaraf R, Leisi R, Ros C.  A Conserved Receptor-Binding Domain in the VP1u of Primate Erythroparvoviruses Determines the Marked Tropism for Erythroid Cells  Viruses  2022  14(2):420  PubMed ID: 35216013   DOI: 10.3390/v14020420

20255  Doerfler PA, Feng R, Li Y, Palmer LE, Porter SN, Bell HW, Crossley M, Pruett-Miller SM, Cheng Y, Weiss MJ.  Activation of γ-globin gene expression by GATA1 and NF-Y in hereditary persistence of fetal hemoglobin.  Nat Genet  2021  53(8):1177-1186  PubMed ID: 34341563   DOI: 10.1038/s41588-021-00904-0

16055  Liu N, Xu S, Yao Q, Zhu Q, Kai Y, Hsu JY, Sakon P, Pinello L, Yuan GC, Bauer DE, Orkin SH.  Transcription factor competition at the γ-globin promoters controls hemoglobin switching  Nat Genet  2021  53(4):511-520  PubMed ID: 33649594   DOI: 10.1038/s41588-021-00798-y

16059  Cheng L, Li Y, Qi Q, Xu P, Feng R, Palmer L, Chen J, Wu R, Yee T, Zhang J, Yao Y, Sharma A, Hardison RC, Weiss MJ, Cheng Y.  Single-nucleotide-level mapping of DNA regulatory elements that control fetal hemoglobin expression  Nat Genet   2021  53(6):869-880  PubMed ID: 33958780   DOI: 10.1038/s41588-021-00861-8

16062  Demers M, Sturtevant S, Guertin KR, Gupta D, Desai K, Vieira BF, Li W, Hicks A, Ismail A, Gonçalves BP, Di Caprio G, Schonbrun E, Hansen S, Musayev FN, Safo MK, Wood DK, Higgins JM, Light DR.  MetAP2 inhibition modifies hemoglobin S to delay polymerization and improves blood flow in sickle cell disease  Blood Adv . 2021  2021  1388-1402  PubMed ID: 33661300   DOI: 10.1182/bloodadvances.2020003670

18490  Boontanrart MY, Schröder MS, Stehli GM, Banović M, Wyman SK, Lew RJ, Bordi M, Gowen BG, DeWitt MA, Corn JE.  ATF4 Regulates MYB to Increase γ-Globin in Response to Loss of β-Globin  Cell Rep  2020  32(5):107993  PubMed ID: 32755585   DOI: 10.1016/j.celrep.2020.107993

6780  Makino T, Haruyama M, Katayama K, Terashima H, Tsunemi T, Miyazaki K, Terakawa M, Yamashiro K, Yoshioka R, Maeda H.  Phenotypic-screening generates active novel fetal globin-inducers that downregulate Bcl11a in a monkey model.  Biochem Pharmacol  2020  171:113717  PubMed ID: 31751536   DOI: 10.1016/j.bcp.2019.113717

6746  Dai Y, Shaikho EM, Perez J, Wilson CA, Liu LY, White MR, Farrell JJ, Chui DHK, Sebastiani P, Steinberg MH.  BCL2L1 is associated with γ-globin gene expression.  Blood Adv  2019  3(20):2995-3001  PubMed ID: 31648320   DOI: 10.1182/bloodadvances.2019032243

6766  Mudit Chaand, PhD , Chris Fiore, PhD , Brian T Johnston, MS , Diane H Moon, PhD , John P Carulli, PhD , Jeffrey R Shearstone, PhD  Chromatin Accessibility Mapping of Primary Erythroid Cell Populations Leads to Identification and Validation of Nuclear Factor I X (NFIX) As a Novel Fetal Hemoglobin (HbF) Repressor  Blood  2019  134 (Supplement_1): 812    DOI: 10.1182/blood-2019-124337

4518  Vinjamur DS, Bauer DE  Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines.  Erythropoiesis  2018  275-284  PubMed ID: 29076097   DOI: 10.1007/978-1-4939-7428-3_17

6812  Leung A, Zulick E, Skvir N, Vanuytsel K, Morrison TA, Naing ZH, Wang Z, Dai Y, Chui DHK, Steinberg MH, Sherr DH, Murphy GJ.  Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells.  Stem Cells  2018  36(7):1004-1019  PubMed ID: 29569827   DOI: 10.1002/stem.2822

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利用者成果(日)