Cell No. : Cell Name
RCB4556 : HUDEP-1
update : 2022/12/16
|
| Comment | Human erythroid progenitor cell line derived from umbilical cord blood cells. Expressing fetal type hemoglobin. |
| Comment from the depositor | |
| Terms and conditions | 1) BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. Regarding for-profit use and/or use by for-profit organization please contact us (cellbank.brc@riken.jp). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (PLoS One. 2013;8(3):e59890) designated by the DEPOSITOR is required.
|
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
KURITA, Ryo
|
| Originator |
KURITA, Ryo
|
| Year of deposit |
2013
|
| Cloning (depositor) |
No
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Tissue |
umbilical cord blood
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
KUSABIRA-ORANGE, HPV16 E6/E7
|
| Vector |
CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_VI05
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Suspension cells
|
Suspension cells
|
| Medium and additives |
StemSpan SFEM (Stemcell technologies), EPO(3U/ml), SCF(50ng/ml), DEX(10-6M), DOX(1ug/ml)
|
StemSpan SFEM + 3U/ml EPO + 50ng/ml SCF + 10^(-6)M Dexamethasone + 1ug/ml Doxycycline
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 4-6 split
|
| SC frequency |
Subculture : 2 times/week
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
CELLBANKER 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
10
|
| User's Publication |
3
|
| Reference |
|
15517
Papasavva PL, Papaioannou NY, Patsali P, Kurita R, Nakamura Y, Sitarou M, Christou S, Kleanthous M, Lederer CW.
Distinct miRNA Signatures and Networks Discern Fetal from Adult Erythroid Differentiation and Primary from Immortalized Erythroid Cells
Int J Mol Sci
2021
22(7):3626.
PubMed ID: 33807258
DOI: 10.3390/ijms22073626
|
|
16066
Wessels MW, Cnossen MH, van Dijk TB, Gillemans N, Schmidt KLJ, van Lom K, Vinjamur DS, Coyne S, Kurita R, Nakamura Y, de Man SA, Pfundt R, Azmani Z, Brouwer RWW, Bauer DE, van den Hout MCGN, van IJcken WFJ, Philipsen S.
Molecular analysis of the erythroid phenotype of a patient with BCL11A haploinsufficiency
Blood Adv
2021
5(9):2339-2349
PubMed ID: 33938942
DOI: 10.1182/bloodadvances.2020003753
|
|
8969
Cristina Fugazza, Gloria Barbarani, Sudharshan Elangovan, Maria Giuseppina Marini, Serena Giolitto, Isaura Font-Monclus, Maria Franca Marongiu, Laura Manunza, John Strouboulis, Claudio Cantù, Fabio Gasparri, Silvia M L Barabino, Yukio Nakamura, Sergio Ottolenghi, Paolo Moi, Antonella Ellena Ronchi
The Coup-TFII Orphan Nuclear Receptor Is an Activator of the γ-Globin Gene
Haematologica
2020
106(2):474-482
PubMed ID: 32107331
DOI: 10.3324/haematol.2019.241224
|
|
6752
Svetlana Soboleva , Ryo Kurita, PhD , Fredrik Ek, PhD , Rita Alves , Roger Olsson, PhD , Yukio Nakamura, MD PhD , Kenichi Miharada, PhD
Identification of Potential Chemical Compounds Able to Trigger Enucleation of Immortalized Human Erythroid Cell Lines
Blood
2019
134 (Supplement_1): 951
DOI: 10.1182/blood-2019-128427
|
|
6770
Melanie Demers, PhD , Sarah Sturtevant, BSc , Kevin Guertin, PhD , Dipti Gupta , Kunal Desai, MS , Benjamin Vieira, BS , Alexandra Hicks, PhD , Ayman Ismail, PhD , Yukio Nakamura, MD PhD , Bronner Goncalves, MD PhD , Giuseppe Di Caprio, PhD , Ethan Schonbrun, PhD , Scott Hansen, BS , Martin K. Safo, PhD , David K. Wood, PhD , John M. Higgins, MD PhD , David R. Light, PhD
MetAP2 Inhibition Modifies Hemoglobin S (HbS) to Delay Polymerization and Improve Blood Flow in Sickle Cell Disease
Blood
2019
134 (Supplement_1): 2260
|
|
4974
Morrison TA, Wilcox I, Luo HY, Farrell JJ, Kurita R, Nakamura Y, Murphy GJ, Cui S, Steinberg MH, Chui DHK.
A long noncoding RNA from the HBS1L-MYB intergenic region on chr6q23 regulates human fetal hemoglobin expression.
Blood Cells Mol Dis
2018
69:1-9
PubMed ID: 29227829
DOI: 10.1016/j.bcmd.2017.11.003
|
|
6761
Lee E, Sivalingam J, Lim ZR, Chia G, Shi LG, Roberts M, Loh YH, Reuveny S, Oh SK.
Review: In vitro generation of red blood cells for transfusion medicine: Progress, prospects and challenges.
Biotechnol Adv
2018
36(8):2118-2128
PubMed ID: 30273713
DOI: 10.1016/j.biotechadv.2018.09.006
|
|
6750
Trakarnsanga K, Griffiths RE, Wilson MC, Blair A, Satchwell TJ, Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ, Frayne J.
An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
Nat Commun
2017
8:14750
PubMed ID: 28290447
DOI: 10.1038/ncomms14750
|
|
8824
Yan Dai, PhD , Tyngwei Chen , Heba Ijaz , Elizabeth Cho , Ryo Kurita, PhD , Yukio Nakamura, MD PhD , Martin H. Steinberg, MD
SIRT1 Increases the Expression of Fetal Hemoglobin Genes in Human Adult and Cord Blood Erythroblasts
Blood
2017
130 (Supplement 1): 287
DOI: 10.1182/blood.V130.Suppl_1.287.287
|
|
6742
Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y.
Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.
PLoS One
2013
8(3):e59890
PubMed ID: 23533656
DOI: 10.1371/journal.pone.0059890
|
| User's Publication |
|
16055
Liu N, Xu S, Yao Q, Zhu Q, Kai Y, Hsu JY, Sakon P, Pinello L, Yuan GC, Bauer DE, Orkin SH.
Transcription factor competition at the γ-globin promoters controls hemoglobin switching
Nat Genet
2021
53(4):511-520
PubMed ID: 33649594
DOI: 10.1038/s41588-021-00798-y
|
|
6746
Dai Y, Shaikho EM, Perez J, Wilson CA, Liu LY, White MR, Farrell JJ, Chui DHK, Sebastiani P, Steinberg MH.
BCL2L1 is associated with γ-globin gene expression.
Blood Adv
2019
3(20):2995-3001
PubMed ID: 31648320
DOI: 10.1182/bloodadvances.2019032243
|
|
6766
Mudit Chaand, PhD , Chris Fiore, PhD , Brian T Johnston, MS , Diane H Moon, PhD , John P Carulli, PhD , Jeffrey R Shearstone, PhD
Chromatin Accessibility Mapping of Primary Erythroid Cell Populations Leads to Identification and Validation of Nuclear Factor I X (NFIX) As a Novel Fetal Hemoglobin (HbF) Repressor
Blood
2019
134 (Supplement_1): 812
DOI: 10.1182/blood-2019-124337
|
Back
Top
CELL BANK Website