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Cell No. : Cell Name
RCB4556 : HUDEP-1  update : 2024/07/11
CommentHuman erythroid progenitor cell line derived from umbilical cord blood cells. Expressing fetal type hemoglobin.
Comment from the depositor
Terms and conditions1) BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. Regarding for-profit use and/or use by for-profit organization please contact us ( 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (PLoS One. 2013;8(3):e59890) designated by the DEPOSITOR is required.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information IPR RIKEN BioResource Research Center (Japan)
Depositor KURITA, Ryo
Originator KURITA, Ryo
Year of deposit 2013
Cloning (depositor) No
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Tissue umbilical cord blood
Classification transformed
Recombinant recombinant
Vector CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_VI05
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium StemSpan SFEM (Stemcell technologies), EPO(3U/ml), SCF(50ng/ml), DEX(10-6M), DOX(1ug/ml) StemSpan SFEM + 3U/ml EPO + 50ng/ml SCF + 10 -6M Dexamethasone + 1ug/ml Doxycycline
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4-6 split
SC frequency Subculture : 2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) Undetected
Virus (HTLV-1) (-)
STR(human) OK
Reference information Reference 15
User's Publication 18

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18551  Singpant P, Tubsuwan A, Sakdee S, Ketterman AJ, Jearawiriyapaisarn N, Kurita R, Nakamura Y, Songdej D, Tangprasittipap A, Bhukhai K, Chiangjong W, Hongeng S, Saisawang C.  Recombinant Cas9 protein production in an endotoxin-free system and evaluation with editing the BCL11A gene in human cells  Protein Expr Purif  2023  210:106313  PubMed ID: 37276914   DOI: 10.1016/j.pep.2023.106313
18555  Valli R, Penzo M.  Editorial: Inherited and acquired ribosomopathies: missing puzzle pieces  Front Genet  2023  14:1194788  PubMed ID: 37124624   DOI: 10.3389/fgene.2023.1194788
18517  Mukherjee M, Rahaman M, Ray SK, Shukla PC, Dolai TK, Chakravorty N.  Revisiting fetal hemoglobin inducers in beta-hemoglobinopathies: a review of natural products, conventional and combinatorial therapies  Mol Biol Rep  2022  49(3):2359-2373  PubMed ID: 34822068   DOI: 10.1007/s11033-021-06977-8
18550  Piantanida N, La Vecchia M, Sculco M, Talmon M, Palattella G, Kurita R, Nakamura Y, Ronchi AE, Dianzani I, Ellis SR, Fresu LG, Aspesi A.  Deficiency of ribosomal protein S26, which is mutated in a subset of patients with Diamond Blackfan anemia, impairs erythroid differentiation  Front Genet  2022  13:1045236  PubMed ID: 36579335   DOI: 10.3389/fgene.2022.1045236
17722  Soboleva S, Kurita R, Ek F, Åkerstrand H, Silvério-Alves R, Olsson R, Nakamura Y, Miharada K.  Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines  Commun Biol  2021  4(1):677  PubMed ID: 34083702   DOI: 10.1038/s42003-021-02202-1
15517  Papasavva PL, Papaioannou NY, Patsali P, Kurita R, Nakamura Y, Sitarou M, Christou S, Kleanthous M, Lederer CW.  Distinct miRNA Signatures and Networks Discern Fetal from Adult Erythroid Differentiation and Primary from Immortalized Erythroid Cells  Int J Mol Sci  2021  22(7):3626.  PubMed ID: 33807258   DOI: 10.3390/ijms22073626
16066  Wessels MW, Cnossen MH, van Dijk TB, Gillemans N, Schmidt KLJ, van Lom K, Vinjamur DS, Coyne S, Kurita R, Nakamura Y, de Man SA, Pfundt R, Azmani Z, Brouwer RWW, Bauer DE, van den Hout MCGN, van IJcken WFJ, Philipsen S.  Molecular analysis of the erythroid phenotype of a patient with BCL11A haploinsufficiency  Blood Adv  2021  5(9):2339-2349  PubMed ID: 33938942   DOI: 10.1182/bloodadvances.2020003753
18553  Thalia Papayannopoulou  Control of fetal globin expression in man: new opportunities to challenge past discoveries  Exp Hematol  2020  92:43-50  PubMed ID: 32976950   DOI: 10.1016/j.exphem.2020.09.195
6752  Svetlana Soboleva , Ryo Kurita, PhD , Fredrik Ek, PhD , Rita Alves , Roger Olsson, PhD , Yukio Nakamura, MD PhD , Kenichi Miharada, PhD  Identification of Potential Chemical Compounds Able to Trigger Enucleation of Immortalized Human Erythroid Cell Lines  Blood  2019  134 (Supplement_1): 951    DOI: 10.1182/blood-2019-128427
6770  Melanie Demers, PhD , Sarah Sturtevant, BSc , Kevin Guertin, PhD , Dipti Gupta , Kunal Desai, MS , Benjamin Vieira, BS , Alexandra Hicks, PhD , Ayman Ismail, PhD , Yukio Nakamura, MD PhD , Bronner Goncalves, MD PhD , Giuseppe Di Caprio, PhD , Ethan Schonbrun, PhD , Scott Hansen, BS , Martin K. Safo, PhD , David K. Wood, PhD , John M. Higgins, MD PhD , David R. Light, PhD  MetAP2 Inhibition Modifies Hemoglobin S (HbS) to Delay Polymerization and Improve Blood Flow in Sickle Cell Disease  Blood  2019  134 (Supplement_1): 2260    DOI: 10.1182/blood-2019-128727
4974  Morrison TA, Wilcox I, Luo HY, Farrell JJ, Kurita R, Nakamura Y, Murphy GJ, Cui S, Steinberg MH, Chui DHK.  A long noncoding RNA from the HBS1L-MYB intergenic region on chr6q23 regulates human fetal hemoglobin expression.  Blood Cells Mol Dis  2018  69:1-9  PubMed ID: 29227829   DOI: 10.1016/j.bcmd.2017.11.003
6761  Lee E, Sivalingam J, Lim ZR, Chia G, Shi LG, Roberts M, Loh YH, Reuveny S, Oh SK.  Review: In vitro generation of red blood cells for transfusion medicine: Progress, prospects and challenges.  Biotechnol Adv  2018  36(8):2118-2128  PubMed ID: 30273713   DOI: 10.1016/j.biotechadv.2018.09.006
6750  Trakarnsanga K, Griffiths RE, Wilson MC, Blair A, Satchwell TJ, Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ, Frayne J.  An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.  Nat Commun  2017  8:14750  PubMed ID: 28290447   DOI: 10.1038/ncomms14750
8824  Yan Dai, PhD , Tyngwei Chen , Heba Ijaz , Elizabeth Cho , Ryo Kurita, PhD , Yukio Nakamura, MD PhD , Martin H. Steinberg, MD  SIRT1 Increases the Expression of Fetal Hemoglobin Genes in Human Adult and Cord Blood Erythroblasts  Blood  2017  130 (Supplement 1): 287    DOI: 10.1182/blood.V130.Suppl_1.287.287
6742  Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y.  Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.  PLoS One  2013  8(3):e59890  PubMed ID: 23533656   DOI: 10.1371/journal.pone.0059890

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User's Publication
18552  Li D, Zhao XY, Zhou S, Hu Q, Wu F, Lee HY.  Multidimensional profiling reveals GATA1-modulated stage-specific chromatin states and functional associations during human erythropoiesis  Nucleic Acids Res  2023  51(13):6634-6653  PubMed ID: 37254808   DOI: 10.1093/nar/gkad468
21274  Chaand M, Fiore C, Johnston B, D'Ippolito A, Moon DH, Carulli JP, Shearstone JR.  Erythroid lineage chromatin accessibility maps facilitate identification and validation of NFIX as a fetal hemoglobin repressor.  Commun Biol  2023  6(1):640  PubMed ID: 37316562   DOI: 10.1038/s42003-023-05025-4
21304  Mayuranathan T, Newby GA, Feng R, Yao Y, Mayberry KD, Lazzarotto CR, Li Y, Levine RM, Nimmagadda N, Dempsey E, Kang G, Porter SN, Doerfler PA, Zhang J, Jang Y, Chen J, Bell HW, Crossley M, Bhoopalan SV, Sharma A, Tisdale JF, Pruett-Miller SM, Cheng Y, Tsai SQ, Liu DR, Weiss MJ, Yen JS.  Potent and uniform fetal hemoglobin induction via base editing.  Nat Genet  2023    PubMed ID: 37400614   DOI: 10.1038/s41588-023-01434-7
17720  Wang D, Tanaka-Yano M, Meader E, Kinney MA, Morris V, Lummertz da Rocha E, Liu N, Liu T, Zhu Q, Orkin SH, North TE, Daley GQ, Rowe RG.  Developmental maturation of the hematopoietic system controlled by a Lin28b-let-7-Cbx2 axis  Cell Rep  2022  39(1):110587  PubMed ID: 35385744   DOI: 10.1016/j.celrep.2022.110587
18435  Sun Y, Habara A, Le CQ, Nguyen N, Chen R, Murphy GJ, Chui DHK, Steinberg MH, Cui S.  Pharmacologic induction of PGC-1α stimulates fetal haemoglobin gene expression  Br J Haematol  2022  197(1):97-109  PubMed ID: 35118652   DOI: 10.1111/bjh.18042
18443  Mehta S, Buyanbat A, Kai Y, Karayel O, Goldman SR, Seruggia D, Zhang K, Fujiwara Y, Donovan KA, Zhu Q, Yang H, Nabet B, Gray NS, Mann M, Fischer ES, Adelman K, Orkin SH.  Temporal resolution of gene derepression and proteome changes upon PROTAC-mediated degradation of BCL11A protein in erythroid cells  Cell Chem Biol  2022  29(8):1273-1287  PubMed ID: 35839780   DOI: 10.1016/j.chembiol.2022.06.007
18554  Pavan AR, Lopes JR, Dos Santos JL.  The state of the art of fetal hemoglobin-inducing agents  Expert Opin Drug Discov  2022  17(11):1279-1293  PubMed ID: 36302760   DOI: 10.1080/17460441.2022.2141708
18564  Bircher C, Bieri J, Assaraf R, Leisi R, Ros C.  A Conserved Receptor-Binding Domain in the VP1u of Primate Erythroparvoviruses Determines the Marked Tropism for Erythroid Cells  Viruses  2022  14(2):420  PubMed ID: 35216013   DOI: 10.3390/v14020420
20255  Doerfler PA, Feng R, Li Y, Palmer LE, Porter SN, Bell HW, Crossley M, Pruett-Miller SM, Cheng Y, Weiss MJ.  Activation of γ-globin gene expression by GATA1 and NF-Y in hereditary persistence of fetal hemoglobin.  Nat Genet  2021  53(8):1177-1186  PubMed ID: 34341563   DOI: 10.1038/s41588-021-00904-0
16055  Liu N, Xu S, Yao Q, Zhu Q, Kai Y, Hsu JY, Sakon P, Pinello L, Yuan GC, Bauer DE, Orkin SH.  Transcription factor competition at the γ-globin promoters controls hemoglobin switching  Nat Genet  2021  53(4):511-520  PubMed ID: 33649594   DOI: 10.1038/s41588-021-00798-y
16059  Cheng L, Li Y, Qi Q, Xu P, Feng R, Palmer L, Chen J, Wu R, Yee T, Zhang J, Yao Y, Sharma A, Hardison RC, Weiss MJ, Cheng Y.  Single-nucleotide-level mapping of DNA regulatory elements that control fetal hemoglobin expression  Nat Genet   2021  53(6):869-880  PubMed ID: 33958780   DOI: 10.1038/s41588-021-00861-8
16062  Demers M, Sturtevant S, Guertin KR, Gupta D, Desai K, Vieira BF, Li W, Hicks A, Ismail A, Gonçalves BP, Di Caprio G, Schonbrun E, Hansen S, Musayev FN, Safo MK, Wood DK, Higgins JM, Light DR.  MetAP2 inhibition modifies hemoglobin S to delay polymerization and improves blood flow in sickle cell disease  Blood Adv . 2021  2021  1388-1402  PubMed ID: 33661300   DOI: 10.1182/bloodadvances.2020003670
18490  Boontanrart MY, Schröder MS, Stehli GM, Banović M, Wyman SK, Lew RJ, Bordi M, Gowen BG, DeWitt MA, Corn JE.  ATF4 Regulates MYB to Increase γ-Globin in Response to Loss of β-Globin  Cell Rep  2020  32(5):107993  PubMed ID: 32755585   DOI: 10.1016/j.celrep.2020.107993
6780  Makino T, Haruyama M, Katayama K, Terashima H, Tsunemi T, Miyazaki K, Terakawa M, Yamashiro K, Yoshioka R, Maeda H.  Phenotypic-screening generates active novel fetal globin-inducers that downregulate Bcl11a in a monkey model.  Biochem Pharmacol  2020  171:113717  PubMed ID: 31751536   DOI: 10.1016/j.bcp.2019.113717
6746  Dai Y, Shaikho EM, Perez J, Wilson CA, Liu LY, White MR, Farrell JJ, Chui DHK, Sebastiani P, Steinberg MH.  BCL2L1 is associated with γ-globin gene expression.  Blood Adv  2019  3(20):2995-3001  PubMed ID: 31648320   DOI: 10.1182/bloodadvances.2019032243
6766  Mudit Chaand, PhD , Chris Fiore, PhD , Brian T Johnston, MS , Diane H Moon, PhD , John P Carulli, PhD , Jeffrey R Shearstone, PhD  Chromatin Accessibility Mapping of Primary Erythroid Cell Populations Leads to Identification and Validation of Nuclear Factor I X (NFIX) As a Novel Fetal Hemoglobin (HbF) Repressor  Blood  2019  134 (Supplement_1): 812    DOI: 10.1182/blood-2019-124337
4518  Vinjamur DS, Bauer DE  Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines.  Erythropoiesis  2018  275-284  PubMed ID: 29076097   DOI: 10.1007/978-1-4939-7428-3_17
6812  Leung A, Zulick E, Skvir N, Vanuytsel K, Morrison TA, Naing ZH, Wang Z, Dai Y, Chui DHK, Steinberg MH, Sherr DH, Murphy GJ.  Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells.  Stem Cells  2018  36(7):1004-1019  PubMed ID: 29569827   DOI: 10.1002/stem.2822

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