Cell No. : Cell Name
RCB4555 : HiDEP-2
update : 2024/11/11
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| Comment | Human erythroid progenitor cell line derived from iPS cells. Expressing fetal type hemoglobin. |
| Comment from the depositor | |
| Terms and conditions | 1) BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. Regarding for-profit use and/or use by for-profit organization please contact us (cellbank.brc@riken.jp). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (PLoS One. 2013;8(3):e59890) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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IPR |
RIKEN BioResource Research Center (Japan)
|
| Depositor |
KURITA, Ryo
|
| Originator |
KURITA, Ryo
|
| Year of deposit |
2013
|
| Original cell |
HPS0015 HiPS-RIKEN-3A
|
| Cloning (depositor) |
No
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Female
|
| Tissue |
amniotic membrane
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
OCT3/4, SOX2, c-MYC, KLF-4, TAL1, KUSABIRA-ORANGE, HPV16 E6E7
|
| Vector |
CSII-EF-TAL1-IRES-Puro lentiviral vector, CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector
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|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_VI04
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Suspension cells
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
StemSpan SFEM (Stemcell technologies), EPO(3U/ml), DEX(10-6M), DOX(1ug/ml)
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StemSpan SFEM + 3 U/ml EPO + 10 -6M Dexamethasone + 1ug/ml Doxycycline
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| Antibiotics |
|
Free
|
|
Passage method |
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dilution
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Culture information
|
Passage ratio |
|
1 : 4 split
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| SC frequency |
Subculture : 2 times/week
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
CELLBANKER 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
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| STR(human) |
|
OK
|
| Reference information |
Reference |
12
|
| User's Publication |
2
|
| Reference |
|
18563
Kim S, Jo KW, Park JM, Shin A, Kurita R, Nakamura Y, Kweon S, Baek EJ.
Irradiation is not sufficient to eradicate residual immortalized erythroid cells in in vitro-generated red blood cell products
Transfusion
2023
63(6):1122-1128
PubMed ID: 37154531
DOI: 10.1111/trf.17394
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18358
Suzuki C, Fujiwara T, Shima H, Ono K, Saito K, Kato H, Onodera K, Ichikawa S, Fukuhara N, Onishi Y, Yokoyama H, Nakamura Y, Igarashi K, Harigae H.
Elucidation of the Role of FAM210B in Mitochondrial Metabolism and Erythropoiesis
Mol Cell Biol
2022
42(12):e0014322
PubMed ID: 36374104
DOI: 10.1128/mcb.00143-22
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17722
Soboleva S, Kurita R, Ek F, Åkerstrand H, Silvério-Alves R, Olsson R, Nakamura Y, Miharada K.
Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines
Commun Biol
2021
4(1):677
PubMed ID: 34083702
DOI: 10.1038/s42003-021-02202-1
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6752
Svetlana Soboleva , Ryo Kurita, PhD , Fredrik Ek, PhD , Rita Alves , Roger Olsson, PhD , Yukio Nakamura, MD PhD , Kenichi Miharada, PhD
Identification of Potential Chemical Compounds Able to Trigger Enucleation of Immortalized Human Erythroid Cell Lines
Blood
2019
134 (Supplement_1): 951
DOI: 10.1182/blood-2019-128427
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6753
Chie Suzuki , Tohru Fujiwara, MD PhD , Hiroki Shima , Koya Ono, MD , Kei Saito , Noriko Fukuhara, MD PhD , Yasushi Onishi, MD PhD , Hisayuki Yokoyama, MD PhD , Shinichi Fujimaki , Yukio Nakamura, MD PhD , Hideo Harigae, MD PhD
Proteomic Analysis of Mitochondrial Membrane Protein FAM210B in Erythroid Cells
Blood
2019
134 (Supplement_1): 3523
|
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6757
Saito K, Fujiwara T, Hatta S, Morita M, Ono K, Suzuki C, Fukuhara N, Onishi Y, Nakamura Y, Kawamata S, Shimizu R, Yamamoto M, Harigae H
Generation and Molecular Characterization of Human Ring Sideroblasts: a Key Role of Ferrous Iron in Terminal Erythroid Differentiation and Ring Sideroblast Formation.
Mol Cell Biol
2019
39(7):e00387-18
PubMed ID: 30670569
DOI: 10.1128/MCB.00387-18
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6760
Hansen M, von Lindern M, van den Akker E, Varga E.
Human-induced pluripotent stem cell-derived blood products: state of the art and future directions.
FEBS Lett
2019
593(23):3288-3303
PubMed ID: 31520530
DOI: 10.1002/1873-3468.13599
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6761
Lee E, Sivalingam J, Lim ZR, Chia G, Shi LG, Roberts M, Loh YH, Reuveny S, Oh SK.
Review: In vitro generation of red blood cells for transfusion medicine: Progress, prospects and challenges.
Biotechnol Adv
2018
36(8):2118-2128
PubMed ID: 30273713
DOI: 10.1016/j.biotechadv.2018.09.006
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6750
Trakarnsanga K, Griffiths RE, Wilson MC, Blair A, Satchwell TJ, Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ, Frayne J.
An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
Nat Commun
2017
8:14750
PubMed ID: 28290447
DOI: 10.1038/ncomms14750
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6755
Kondo A, Fujiwara T, Okitsu Y, Fukuhara N, Onishi Y, Nakamura Y, Sawada K, Harigae H.
Identification of a novel putative mitochondrial protein FAM210B associated with erythroid differentiation.
Int J Hematol
2016
103(4):387-95
PubMed ID: 26968549
DOI: 10.1007/s12185-016-1968-4
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6748
Fujiwara T, Okamoto K, Niikuni R, Takahashi K, Okitsu Y, Fukuhara N, Onishi Y, Ishizawa K, Ichinohasama R, Nakamura Y, Nakajima M, Tanaka T, Harigae H.
Effect of 5-aminolevulinic acid on erythropoiesis: a preclinical in vitro characterization for the treatment of congenital sideroblastic anemia.
Biochem Biophys Res Commun
2014
454(1):102-8
PubMed ID: 25450364
DOI: 10.1016/j.bbrc.2014.10.050
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6742
Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y.
Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.
PLoS One
2013
8(3):e59890
PubMed ID: 23533656
DOI: 10.1371/journal.pone.0059890
|
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