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Cell No. : Cell Name
RCB4555 : HiDEP-2  update : 2020/07/09
CommentHuman erythroid progenitor cell line derived from iPS cells. Expressing fetal type hemoglobin.
Comment from the depositor
Terms and conditions1) BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. Regarding for-profit use and/or use by for-profit organization please contact us (cellbank.brc@riken.jp). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (PLoS One. 2013;8(3):e59890) designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor KURITA, Ryo
Originator KURITA, Ryo
Year of deposit 2013
Original cell HPS0015 HiPS-RIKEN-3A
Cloning (depositor) No
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue amniotic membrane
Classification transformed
Recombinant recombinant
Exogene OCT3/4, SOX2, c-MYC, KLF-4, TAL1, KUSABIRA-ORANGE, HPV16 E6E7
Vector CSII-EF-TAL1-IRES-Puro lentiviral vector, CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Medium and additives StemSpan SFEM (Stemcell technologies), EPO(3U/ml), DEX(10-6M), DOX(1ug/ml) StemSpan SFEM +3U/ml EPO + 10^(-6)M Dexamethasone + 1ug/ml Doxycycline
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Animal PCR OK
Reference information Reference 9
User's Publication 1


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Reference
6752  Svetlana Soboleva , Ryo Kurita, PhD , Fredrik Ek, PhD , Rita Alves , Roger Olsson, PhD , Yukio Nakamura, MD PhD , Kenichi Miharada, PhD  Identification of Potential Chemical Compounds Able to Trigger Enucleation of Immortalized Human Erythroid Cell Lines  Blood  2019  134 (Supplement_1): 951 
6753  Chie Suzuki , Tohru Fujiwara, MD PhD , Hiroki Shima , Koya Ono, MD , Kei Saito , Noriko Fukuhara, MD PhD , Yasushi Onishi, MD PhD , Hisayuki Yokoyama, MD PhD , Shinichi Fujimaki , Yukio Nakamura, MD PhD , Hideo Harigae, MD PhD  Proteomic Analysis of Mitochondrial Membrane Protein FAM210B in Erythroid Cells   Blood  2019  134 (Supplement_1): 3523 
6757  Saito K, Fujiwara T, Hatta S, Morita M, Ono K, Suzuki C, Fukuhara N, Onishi Y, Nakamura Y, Kawamata S, Shimizu R, Yamamoto M, Harigae H  Generation and Molecular Characterization of Human Ring Sideroblasts: a Key Role of Ferrous Iron in Terminal Erythroid Differentiation and Ring Sideroblast Formation.  Mol Cell Biol  2019  39(7):e00387-18  PubMed ID: 30670569
6760  Hansen M, von Lindern M, van den Akker E, Varga E.  Human-induced pluripotent stem cell-derived blood products: state of the art and future directions.  FEBS Lett  2019  593(23):3288-3303  PubMed ID: 31520530
6761  Lee E, Sivalingam J, Lim ZR, Chia G, Shi LG, Roberts M, Loh YH, Reuveny S, Oh SK.  Review: In vitro generation of red blood cells for transfusion medicine: Progress, prospects and challenges.  Biotechnol Adv  2018  36(8):2118-2128  PubMed ID: 30273713
6750  Trakarnsanga K, Griffiths RE, Wilson MC, Blair A, Satchwell TJ, Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ, Frayne J.  An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.  Nat Commun  2017  8:14750  PubMed ID: 28290447
6755  Kondo A, Fujiwara T, Okitsu Y, Fukuhara N, Onishi Y, Nakamura Y, Sawada K, Harigae H.  Identification of a novel putative mitochondrial protein FAM210B associated with erythroid differentiation.  Int J Hematol  2016  103(4):387-95  PubMed ID: 26968549
6748  Fujiwara T, Okamoto K, Niikuni R, Takahashi K, Okitsu Y, Fukuhara N, Onishi Y, Ishizawa K, Ichinohasama R, Nakamura Y, Nakajima M, Tanaka T, Harigae H.  Effect of 5-aminolevulinic acid on erythropoiesis: a preclinical in vitro characterization for the treatment of congenital sideroblastic anemia.  Biochem Biophys Res Commun  2014  454(1):102-8  PubMed ID: 25450364
6742  Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y.  Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.  PLoS One  2013  8(3):e59890  PubMed ID: 23533656

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User's Publication
6758  Inoue A, Fujiwara T, Okitsu Y, Katsuoka Y, Fukuhara N, Onishi Y, Ishizawa K, Harigae H.  Elucidation of the role of LMO2 in human erythroid cells.  Exp Hematol  2013  41(12):1062-76  PubMed ID: 24041784



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