検索画面
細胞材料開発室サイト

戻る 戻る
細胞材料の再培養方法 ( HPS以外・ HPS )
細胞番号 : 細胞名
RCB4554 : HiDEP-1  update : 2024/11/11
細胞特性(Comment:英)Human erythroid progenitor cell line derived from iPS cells. Expressing fetal type hemoglobin.
細胞特性(日)ヒト赤血球前駆細胞株。iPS細胞から分化誘導した赤血球前駆細胞由来。胎児型ヘモグロビン産生株。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)1) BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. Regarding for-profit use and/or use by for-profit organization please contact us (cellbank.brc@riken.jp). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (PLoS One. 2013;8(3):e59890) designated by the DEPOSITOR is required.
使用条件(日)1) 非営利団体による学術研究に限る。営利目的の使用および/または営利団体による使用については、(cellbank.brc@riken.jp) にお問い合わせください。2) 研究成果の公表にあたって寄託者の指定する文献 (PLoS One. 2013;8(3):e59890) を引用すること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 知的財産権 RIKEN BioResource Research Center (Japan)
寄託者 KURITA, Ryo
樹立者 KURITA, Ryo
寄託日 2013
元の細胞 HPS0021 HiPS-RIKEN-4A
クローニング（寄託者） No
動物種 _human < Mammals
属名 Homo
種名 sapiens
人種 Japanese
性別 Female
採取組織 amniotic membrane
細胞分類 transformed
遺伝子改変 recombinant
外来遺伝子 OCT3/4, SOX2, c-MYC, KLF-4, TAL1, KUSABIRA-ORANGE, HPV16 E6E7
外来遺伝子（ベクター） CSII-EF-TAL1-IRES-Puro lentiviral vector, CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector
細胞寿命 infinite
細胞形態 lymphocyte-like
Cellosaurus(Expasy) CVCL_VI03
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Suspension cells Suspension cells
培養方法 General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
培地 StemSpan SFEM (Stemcell technologies), EPO(3U/ml), DEX(10-6M), DOX(1ug/ml) StemSpan SFEM + 3U/ml EPO + 10 -6M Dexamethasone + 1ug/ml Doxycycline
抗生物質 Free
継代方法 dilution
継代密度 1 : 3 split
継代・培地交換頻度 Subculture : 2 times/week Subculture : 2 times/week
培養最適温度 37 ℃ 37 ℃
二酸化炭素濃度 5 % 5 %
凍結培地 Medium + 10% DMSO CELLBANKER 1
凍結方法 Slow freezing
マイコプラズマ/アコレプラズマ (-)
動物種PCR OK
個体識別検査 OK
文献情報 Reference(英) 20件
Reference(日) 0件
利用者成果(英) 3件
利用者成果(日) 0件

トップへページトップへ
Reference(英)
18563  Kim S, Jo KW, Park JM, Shin A, Kurita R, Nakamura Y, Kweon S, Baek EJ.  Irradiation is not sufficient to eradicate residual immortalized erythroid cells in in vitro-generated red blood cell products  Transfusion  2023  63(6):1122-1128  PubMed ID: 37154531   DOI: 10.1111/trf.17394
17392  Takase S, Hiroyama T, Shirai F, Maemoto Y, Nakata A, Arata M, Matsuoka S, Sonoda T, Niwa H, Sato S, Umehara T, Shirouzu M, Nishigaya Y, Sumiya T, Hashimoto N, Namie R, Usui M, Ohishi T, Ohba SI, Kawada M, Hayashi Y, Harada H, Yamaguchi T, Shinkai Y, Nakamura Y, Yoshida M, Ito A.  A specific G9a inhibitor unveils BGLT3 lncRNA as a universal mediator of chemically induced fetal globin gene expression  Nat Commun  2023  14(1):23.  PubMed ID: 36635268   DOI: 10.1038/s41467-022-35404-0
17721  Soboleva S, Kurita R, Kajitani N, Åkerstrand H, Miharada K.  Establishment of an immortalized human erythroid cell line sustaining differentiation potential without inducible gene expression system  Hum Cell  2022  35(1):408-417  PubMed ID: 34817797   DOI: 10.1007/s13577-021-00652-7
18358  Suzuki C, Fujiwara T, Shima H, Ono K, Saito K, Kato H, Onodera K, Ichikawa S, Fukuhara N, Onishi Y, Yokoyama H, Nakamura Y, Igarashi K, Harigae H.  Elucidation of the Role of FAM210B in Mitochondrial Metabolism and Erythropoiesis  Mol Cell Biol  2022  42(12):e0014322  PubMed ID: 36374104   DOI: 10.1128/mcb.00143-22
20678  Ono K, Fujiwara T, Saito K, Nishizawa H, Takahashi N, Suzuki C, Ochi T, Kato H, Ishii Y, Onodera K, Ichikawa S, Fukuhara N, Onishi Y, Yokoyama H, Yamada R, Nakamura Y, Igarashi K, Harigae H.  Congenital sideroblastic anemia model due to ALAS2 mutation is susceptible to ferroptosis.  Sci Rep  2022  12(1):9024  PubMed ID: 35637209   DOI: 10.1038/s41598-022-12940-9
17722  Soboleva S, Kurita R, Ek F, Åkerstrand H, Silvério-Alves R, Olsson R, Nakamura Y, Miharada K.  Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines  Commun Biol  2021  4(1):677  PubMed ID: 34083702   DOI: 10.1038/s42003-021-02202-1
18553  Thalia Papayannopoulou  Control of fetal globin expression in man: new opportunities to challenge past discoveries  Exp Hematol  2020  92:43-50  PubMed ID: 32976950   DOI: 10.1016/j.exphem.2020.09.195
6752  Svetlana Soboleva , Ryo Kurita, PhD , Fredrik Ek, PhD , Rita Alves , Roger Olsson, PhD , Yukio Nakamura, MD PhD , Kenichi Miharada, PhD  Identification of Potential Chemical Compounds Able to Trigger Enucleation of Immortalized Human Erythroid Cell Lines  Blood  2019  134 (Supplement_1): 951    DOI: 10.1182/blood-2019-128427
6753  Chie Suzuki , Tohru Fujiwara, MD PhD , Hiroki Shima , Koya Ono, MD , Kei Saito , Noriko Fukuhara, MD PhD , Yasushi Onishi, MD PhD , Hisayuki Yokoyama, MD PhD , Shinichi Fujimaki , Yukio Nakamura, MD PhD , Hideo Harigae, MD PhD  Proteomic Analysis of Mitochondrial Membrane Protein FAM210B in Erythroid Cells   Blood  2019  134 (Supplement_1): 3523   
6757  Saito K, Fujiwara T, Hatta S, Morita M, Ono K, Suzuki C, Fukuhara N, Onishi Y, Nakamura Y, Kawamata S, Shimizu R, Yamamoto M, Harigae H  Generation and Molecular Characterization of Human Ring Sideroblasts: a Key Role of Ferrous Iron in Terminal Erythroid Differentiation and Ring Sideroblast Formation.  Mol Cell Biol  2019  39(7):e00387-18  PubMed ID: 30670569   DOI: 10.1128/MCB.00387-18
6760  Hansen M, von Lindern M, van den Akker E, Varga E.  Human-induced pluripotent stem cell-derived blood products: state of the art and future directions.  FEBS Lett  2019  593(23):3288-3303  PubMed ID: 31520530   DOI: 10.1002/1873-3468.13599
6728  Ghosh A, Garee G, Sweeny EA, Nakamura Y, Stuehr DJ.  Hsp90 chaperones hemoglobin maturation in erythroid and nonerythroid cells.  Proc Natl Acad Sci U S A  2018  115(6):E1117-E1126  PubMed ID: 29358373   DOI: 10.1073/pnas.1717993115
6751  Kikuchi G, Kurita R, Ogasawara K, Isa K, Tsuneyama H, Nakamura Y, Yabe R, Shiba M, Tadokoro K, Nagai T, Satake M  Application of immortalized human erythroid progenitor cell line in serologic tests to detect red blood cell alloantibodies.  Transfusion  2018  58(11):2675-2682  PubMed ID: 30180269   DOI: 10.1111/trf.14840
6761  Lee E, Sivalingam J, Lim ZR, Chia G, Shi LG, Roberts M, Loh YH, Reuveny S, Oh SK.  Review: In vitro generation of red blood cells for transfusion medicine: Progress, prospects and challenges.  Biotechnol Adv  2018  36(8):2118-2128  PubMed ID: 30273713   DOI: 10.1016/j.biotechadv.2018.09.006
6750  Trakarnsanga K, Griffiths RE, Wilson MC, Blair A, Satchwell TJ, Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ, Frayne J.  An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.  Nat Commun  2017  8:14750  PubMed ID: 28290447   DOI: 10.1038/ncomms14750
6755  Kondo A, Fujiwara T, Okitsu Y, Fukuhara N, Onishi Y, Nakamura Y, Sawada K, Harigae H.  Identification of a novel putative mitochondrial protein FAM210B associated with erythroid differentiation.  Int J Hematol  2016  103(4):387-95  PubMed ID: 26968549   DOI: 10.1007/s12185-016-1968-4
6756  Kim YH, Kim HO, Baek EJ, Kurita R, Cha HJ, Nakamura Y, Kim H.  Rh D blood group conversion using transcription activator-like effector nucleases.  Nat Commun  2015  6:7451  PubMed ID: 26078220   DOI: 10.1038/ncomms8451
6748  Fujiwara T, Okamoto K, Niikuni R, Takahashi K, Okitsu Y, Fukuhara N, Onishi Y, Ishizawa K, Ichinohasama R, Nakamura Y, Nakajima M, Tanaka T, Harigae H.  Effect of 5-aminolevulinic acid on erythropoiesis: a preclinical in vitro characterization for the treatment of congenital sideroblastic anemia.  Biochem Biophys Res Commun  2014  454(1):102-8  PubMed ID: 25450364   DOI: 10.1016/j.bbrc.2014.10.050
6749  Trakarnsanga K, Wilson MC, Lau W, Singleton BK, Parsons SF, Sakuntanaga P, Kurita R, Nakamura Y, Anstee DJ, Frayne J  Induction of adult levels of β-globin in human erythroid cells that intrinsically express embryonic or fetal globin by transduction with KLF1 and BCL11A-XL.  Haematologica  2014  99(11):1677-85  PubMed ID: 25107887   DOI: 10.3324/haematol.2014.110155
6742  Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y.  Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.  PLoS One  2013  8(3):e59890  PubMed ID: 23533656   DOI: 10.1371/journal.pone.0059890

トップへページトップへ
Reference(日)

トップへページトップへ
利用者成果(英)
18564  Bircher C, Bieri J, Assaraf R, Leisi R, Ros C.  A Conserved Receptor-Binding Domain in the VP1u of Primate Erythroparvoviruses Determines the Marked Tropism for Erythroid Cells  Viruses  2022  14(2):420  PubMed ID: 35216013   DOI: 10.3390/v14020420
18368  Ferguson DCJ, Mokim JH, Meinders M, Moody ERR, Williams TA, Cooke S, Trakarnsanga K, Daniels DE, Ferrer-Vicens I, Shoemark D, Tipgomut C, Macinnes KA, Wilson MC, Singleton BK, Frayne J.  Characterization and evolutionary origin of novel C2H2 zinc finger protein (ZNF648) required for both erythroid and megakaryocyte differentiation in humans  Haematologica  2021  106(11):2859-2873  PubMed ID: 33054117   DOI: 10.3324/haematol.2020.256347
6758  Inoue A, Fujiwara T, Okitsu Y, Katsuoka Y, Fukuhara N, Onishi Y, Ishizawa K, Harigae H.  Elucidation of the role of LMO2 in human erythroid cells.  Exp Hematol  2013  41(12):1062-76  PubMed ID: 24041784   DOI: 10.1016/j.exphem.2013.09.003

トップへページトップへ
利用者成果(日)


戻る 戻る 理研トップページへ