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Cell No. : Cell Name
RCB4554 : HiDEP-1  update : 2025/07/10
CommentHuman erythroid progenitor cell line derived from iPS cells. Expressing fetal type hemoglobin.
Comment from the depositor
Terms and conditions1)BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. Regarding for-profit use and/or use by for-profit organization please contact us (cellbank.brc@riken.jp). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (PLoS One. 2013;8(3):e59890) designated by the DEPOSITOR is required. 3) When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information IPR RIKEN BioResource Research Center (Japan)
Depositor KURITA, Ryo
Originator KURITA, Ryo
Year of deposit 2013
Original cell HPS0021 HiPS-RIKEN-4A
Cloning (depositor) No
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue amniotic membrane
Classification transformed
Recombinant recombinant
Exogene OCT3/4, SOX2, c-MYC, KLF-4, TAL1, KUSABIRA-ORANGE, HPV16 E6E7
Vector CSII-EF-TAL1-IRES-Puro lentiviral vector, CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_VI03
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Culture method General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium StemSpan SFEM (Stemcell technologies), EPO(3U/ml), DEX(10-6M), DOX(1ug/ml) StemSpan SFEM + 3U/ml EPO + 10 -6M Dexamethasone + 1ug/ml Doxycycline
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 3 split
SC frequency Subculture : 2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO CELLBANKER 1
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Virus (HIV) Undetected
Virus (HTLV-1) (-)
STR(human) OK
Reference information Reference 20
User's Publication 3


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Reference
18563  Kim S, Jo KW, Park JM, Shin A, Kurita R, Nakamura Y, Kweon S, Baek EJ.  Irradiation is not sufficient to eradicate residual immortalized erythroid cells in in vitro-generated red blood cell products  Transfusion  2023  63(6):1122-1128  PubMed ID: 37154531   DOI: 10.1111/trf.17394
17392  Takase S, Hiroyama T, Shirai F, Maemoto Y, Nakata A, Arata M, Matsuoka S, Sonoda T, Niwa H, Sato S, Umehara T, Shirouzu M, Nishigaya Y, Sumiya T, Hashimoto N, Namie R, Usui M, Ohishi T, Ohba SI, Kawada M, Hayashi Y, Harada H, Yamaguchi T, Shinkai Y, Nakamura Y, Yoshida M, Ito A.  A specific G9a inhibitor unveils BGLT3 lncRNA as a universal mediator of chemically induced fetal globin gene expression  Nat Commun  2023  14(1):23.  PubMed ID: 36635268   DOI: 10.1038/s41467-022-35404-0
17721  Soboleva S, Kurita R, Kajitani N, Åkerstrand H, Miharada K.  Establishment of an immortalized human erythroid cell line sustaining differentiation potential without inducible gene expression system  Hum Cell  2022  35(1):408-417  PubMed ID: 34817797   DOI: 10.1007/s13577-021-00652-7
18358  Suzuki C, Fujiwara T, Shima H, Ono K, Saito K, Kato H, Onodera K, Ichikawa S, Fukuhara N, Onishi Y, Yokoyama H, Nakamura Y, Igarashi K, Harigae H.  Elucidation of the Role of FAM210B in Mitochondrial Metabolism and Erythropoiesis  Mol Cell Biol  2022  42(12):e0014322  PubMed ID: 36374104   DOI: 10.1128/mcb.00143-22
20678  Ono K, Fujiwara T, Saito K, Nishizawa H, Takahashi N, Suzuki C, Ochi T, Kato H, Ishii Y, Onodera K, Ichikawa S, Fukuhara N, Onishi Y, Yokoyama H, Yamada R, Nakamura Y, Igarashi K, Harigae H.  Congenital sideroblastic anemia model due to ALAS2 mutation is susceptible to ferroptosis.  Sci Rep  2022  12(1):9024  PubMed ID: 35637209   DOI: 10.1038/s41598-022-12940-9
17722  Soboleva S, Kurita R, Ek F, Åkerstrand H, Silvério-Alves R, Olsson R, Nakamura Y, Miharada K.  Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines  Commun Biol  2021  4(1):677  PubMed ID: 34083702   DOI: 10.1038/s42003-021-02202-1
18553  Thalia Papayannopoulou  Control of fetal globin expression in man: new opportunities to challenge past discoveries  Exp Hematol  2020  92:43-50  PubMed ID: 32976950   DOI: 10.1016/j.exphem.2020.09.195
6752  Svetlana Soboleva , Ryo Kurita, PhD , Fredrik Ek, PhD , Rita Alves , Roger Olsson, PhD , Yukio Nakamura, MD PhD , Kenichi Miharada, PhD  Identification of Potential Chemical Compounds Able to Trigger Enucleation of Immortalized Human Erythroid Cell Lines  Blood  2019  134 (Supplement_1): 951    DOI: 10.1182/blood-2019-128427
6753  Chie Suzuki , Tohru Fujiwara, MD PhD , Hiroki Shima , Koya Ono, MD , Kei Saito , Noriko Fukuhara, MD PhD , Yasushi Onishi, MD PhD , Hisayuki Yokoyama, MD PhD , Shinichi Fujimaki , Yukio Nakamura, MD PhD , Hideo Harigae, MD PhD  Proteomic Analysis of Mitochondrial Membrane Protein FAM210B in Erythroid Cells   Blood  2019  134 (Supplement_1): 3523   
6757  Saito K, Fujiwara T, Hatta S, Morita M, Ono K, Suzuki C, Fukuhara N, Onishi Y, Nakamura Y, Kawamata S, Shimizu R, Yamamoto M, Harigae H  Generation and Molecular Characterization of Human Ring Sideroblasts: a Key Role of Ferrous Iron in Terminal Erythroid Differentiation and Ring Sideroblast Formation.  Mol Cell Biol  2019  39(7):e00387-18  PubMed ID: 30670569   DOI: 10.1128/MCB.00387-18
6760  Hansen M, von Lindern M, van den Akker E, Varga E.  Human-induced pluripotent stem cell-derived blood products: state of the art and future directions.  FEBS Lett  2019  593(23):3288-3303  PubMed ID: 31520530   DOI: 10.1002/1873-3468.13599
6728  Ghosh A, Garee G, Sweeny EA, Nakamura Y, Stuehr DJ.  Hsp90 chaperones hemoglobin maturation in erythroid and nonerythroid cells.  Proc Natl Acad Sci U S A  2018  115(6):E1117-E1126  PubMed ID: 29358373   DOI: 10.1073/pnas.1717993115
6751  Kikuchi G, Kurita R, Ogasawara K, Isa K, Tsuneyama H, Nakamura Y, Yabe R, Shiba M, Tadokoro K, Nagai T, Satake M  Application of immortalized human erythroid progenitor cell line in serologic tests to detect red blood cell alloantibodies.  Transfusion  2018  58(11):2675-2682  PubMed ID: 30180269   DOI: 10.1111/trf.14840
6761  Lee E, Sivalingam J, Lim ZR, Chia G, Shi LG, Roberts M, Loh YH, Reuveny S, Oh SK.  Review: In vitro generation of red blood cells for transfusion medicine: Progress, prospects and challenges.  Biotechnol Adv  2018  36(8):2118-2128  PubMed ID: 30273713   DOI: 10.1016/j.biotechadv.2018.09.006
6750  Trakarnsanga K, Griffiths RE, Wilson MC, Blair A, Satchwell TJ, Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ, Frayne J.  An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.  Nat Commun  2017  8:14750  PubMed ID: 28290447   DOI: 10.1038/ncomms14750
6755  Kondo A, Fujiwara T, Okitsu Y, Fukuhara N, Onishi Y, Nakamura Y, Sawada K, Harigae H.  Identification of a novel putative mitochondrial protein FAM210B associated with erythroid differentiation.  Int J Hematol  2016  103(4):387-95  PubMed ID: 26968549   DOI: 10.1007/s12185-016-1968-4
6756  Kim YH, Kim HO, Baek EJ, Kurita R, Cha HJ, Nakamura Y, Kim H.  Rh D blood group conversion using transcription activator-like effector nucleases.  Nat Commun  2015  6:7451  PubMed ID: 26078220   DOI: 10.1038/ncomms8451
6748  Fujiwara T, Okamoto K, Niikuni R, Takahashi K, Okitsu Y, Fukuhara N, Onishi Y, Ishizawa K, Ichinohasama R, Nakamura Y, Nakajima M, Tanaka T, Harigae H.  Effect of 5-aminolevulinic acid on erythropoiesis: a preclinical in vitro characterization for the treatment of congenital sideroblastic anemia.  Biochem Biophys Res Commun  2014  454(1):102-8  PubMed ID: 25450364   DOI: 10.1016/j.bbrc.2014.10.050
6749  Trakarnsanga K, Wilson MC, Lau W, Singleton BK, Parsons SF, Sakuntanaga P, Kurita R, Nakamura Y, Anstee DJ, Frayne J  Induction of adult levels of β-globin in human erythroid cells that intrinsically express embryonic or fetal globin by transduction with KLF1 and BCL11A-XL.  Haematologica  2014  99(11):1677-85  PubMed ID: 25107887   DOI: 10.3324/haematol.2014.110155
6742  Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y.  Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.  PLoS One  2013  8(3):e59890  PubMed ID: 23533656   DOI: 10.1371/journal.pone.0059890

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User's Publication
18564  Bircher C, Bieri J, Assaraf R, Leisi R, Ros C.  A Conserved Receptor-Binding Domain in the VP1u of Primate Erythroparvoviruses Determines the Marked Tropism for Erythroid Cells  Viruses  2022  14(2):420  PubMed ID: 35216013   DOI: 10.3390/v14020420
18368  Ferguson DCJ, Mokim JH, Meinders M, Moody ERR, Williams TA, Cooke S, Trakarnsanga K, Daniels DE, Ferrer-Vicens I, Shoemark D, Tipgomut C, Macinnes KA, Wilson MC, Singleton BK, Frayne J.  Characterization and evolutionary origin of novel C2H2 zinc finger protein (ZNF648) required for both erythroid and megakaryocyte differentiation in humans  Haematologica  2021  106(11):2859-2873  PubMed ID: 33054117   DOI: 10.3324/haematol.2020.256347
6758  Inoue A, Fujiwara T, Okitsu Y, Katsuoka Y, Fukuhara N, Onishi Y, Ishizawa K, Harigae H.  Elucidation of the role of LMO2 in human erythroid cells.  Exp Hematol  2013  41(12):1062-76  PubMed ID: 24041784   DOI: 10.1016/j.exphem.2013.09.003


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