Cell No. : Cell Name
RCB4554 : HiDEP-1
update : 2020/07/09
|
| Comment | Human erythroid progenitor cell line derived from iPS cells. Expressing fetal type hemoglobin. |
| Comment from the depositor | |
| Terms and conditions | 1) BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. Regarding for-profit use and/or use by for-profit organization please contact us (cellbank.brc@riken.jp). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (PLoS One. 2013;8(3):e59890) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
|
Expected time |
Contact us: cellbank.brc@riken.jp
|
| Depositor |
KURITA, Ryo
|
| Originator |
KURITA, Ryo
|
| Year of deposit |
2013
|
| Original cell |
HPS0021 HiPS-RIKEN-4A
|
| Cloning (depositor) |
No
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Female
|
| Tissue |
amniotic membrane
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
OCT3/4, SOX2, c-MYC, KLF-4, TAL1, KUSABIRA-ORANGE, HPV16 E6E7
|
| Vector |
CSII-EF-TAL1-IRES-Puro lentiviral vector, CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
|
| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Suspension cells
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Suspension cells
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| Medium and additives |
StemSpan SFEM (Stemcell technologies), EPO(3U/ml), DEX(10-6M), DOX(1ug/ml)
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StemSpan SFEM +3U/ml EPO + 10^(-6)M Dexamethasone + 1ug/ml Doxycycline
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| Antibiotics |
|
Free
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|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 3 split
|
| SC frequency |
Subculture : 2 times/week
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
CELLBANKER 1
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| Freezing method |
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Slow freezing
|
| Mycoplasma |
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(-)
|
| STR(human) |
|
OK
|
| Animal PCR |
|
OK
|
| Reference information |
Reference |
14
|
| User's Publication |
1
|
| Reference |
|
6752
Svetlana Soboleva , Ryo Kurita, PhD , Fredrik Ek, PhD , Rita Alves , Roger Olsson, PhD , Yukio Nakamura, MD PhD , Kenichi Miharada, PhD
Identification of Potential Chemical Compounds Able to Trigger Enucleation of Immortalized Human Erythroid Cell Lines
Blood
2019
134 (Supplement_1): 951
|
|
6753
Chie Suzuki , Tohru Fujiwara, MD PhD , Hiroki Shima , Koya Ono, MD , Kei Saito , Noriko Fukuhara, MD PhD , Yasushi Onishi, MD PhD , Hisayuki Yokoyama, MD PhD , Shinichi Fujimaki , Yukio Nakamura, MD PhD , Hideo Harigae, MD PhD
Proteomic Analysis of Mitochondrial Membrane Protein FAM210B in Erythroid Cells
Blood
2019
134 (Supplement_1): 3523
|
|
6757
Saito K, Fujiwara T, Hatta S, Morita M, Ono K, Suzuki C, Fukuhara N, Onishi Y, Nakamura Y, Kawamata S, Shimizu R, Yamamoto M, Harigae H
Generation and Molecular Characterization of Human Ring Sideroblasts: a Key Role of Ferrous Iron in Terminal Erythroid Differentiation and Ring Sideroblast Formation.
Mol Cell Biol
2019
39(7):e00387-18
PubMed ID: 30670569
|
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6759
Koya Ono, MD , Tohru Fujiwara, MD PhD , Kei Saito, MD PhD , Chie Suzuki , Noriyuki Takahashi , Koichi Onodera, MD PhD , Satoshi Ichikawa, MD PhD , Noriko Fukuhara, MD PhD , Yasushi Onishi, MD PhD , Hisayuki Yokoyama, MD PhD , Yukio Nakamura, MD PhD , Hideo Harigae, MD PhD
Molecular Characterization and Novel Therapeutic Strategy for X-Linked Sideroblastic Anemia Associated with ALAS2 Missense Variants
Blood
2019
134 (Supplement_1): 3530
|
|
6760
Hansen M, von Lindern M, van den Akker E, Varga E.
Human-induced pluripotent stem cell-derived blood products: state of the art and future directions.
FEBS Lett
2019
593(23):3288-3303
PubMed ID: 31520530
|
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6728
Ghosh A, Garee G, Sweeny EA, Nakamura Y, Stuehr DJ.
Hsp90 chaperones hemoglobin maturation in erythroid and nonerythroid cells.
Proc Natl Acad Sci U S A
2018
115(6):E1117-E1126
PubMed ID: 29358373
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6751
Kikuchi G, Kurita R, Ogasawara K, Isa K, Tsuneyama H, Nakamura Y, Yabe R, Shiba M, Tadokoro K, Nagai T, Satake M
Application of immortalized human erythroid progenitor cell line in serologic tests to detect red blood cell alloantibodies.
Transfusion
2018
58(11):2675-2682
PubMed ID: 30180269
|
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6761
Lee E, Sivalingam J, Lim ZR, Chia G, Shi LG, Roberts M, Loh YH, Reuveny S, Oh SK.
Review: In vitro generation of red blood cells for transfusion medicine: Progress, prospects and challenges.
Biotechnol Adv
2018
36(8):2118-2128
PubMed ID: 30273713
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6750
Trakarnsanga K, Griffiths RE, Wilson MC, Blair A, Satchwell TJ, Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ, Frayne J.
An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
Nat Commun
2017
8:14750
PubMed ID: 28290447
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6755
Kondo A, Fujiwara T, Okitsu Y, Fukuhara N, Onishi Y, Nakamura Y, Sawada K, Harigae H.
Identification of a novel putative mitochondrial protein FAM210B associated with erythroid differentiation.
Int J Hematol
2016
103(4):387-95
PubMed ID: 26968549
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6756
Kim YH, Kim HO, Baek EJ, Kurita R, Cha HJ, Nakamura Y, Kim H.
Rh D blood group conversion using transcription activator-like effector nucleases.
Nat Commun
2015
6:7451
PubMed ID: 26078220
|
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6748
Fujiwara T, Okamoto K, Niikuni R, Takahashi K, Okitsu Y, Fukuhara N, Onishi Y, Ishizawa K, Ichinohasama R, Nakamura Y, Nakajima M, Tanaka T, Harigae H.
Effect of 5-aminolevulinic acid on erythropoiesis: a preclinical in vitro characterization for the treatment of congenital sideroblastic anemia.
Biochem Biophys Res Commun
2014
454(1):102-8
PubMed ID: 25450364
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6749
Trakarnsanga K, Wilson MC, Lau W, Singleton BK, Parsons SF, Sakuntanaga P, Kurita R, Nakamura Y, Anstee DJ, Frayne J
Induction of adult levels of β-globin in human erythroid cells that intrinsically express embryonic or fetal globin by transduction with KLF1 and BCL11A-XL.
Haematologica
2014
99(11):1677-85
PubMed ID: 25107887
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6742
Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y.
Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.
PLoS One
2013
8(3):e59890
PubMed ID: 23533656
|
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