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Cell No. : Cell Name
RCB4549 : RSV-M  update : 2024/07/09
CommentMouse brain tumors induced with Rous sarcom.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor KUMANISHI, Toshiro
Originator SEKIGUCHI, Kentaro
Year of deposit 2013
Another name RSV-mouse brain tumar
Cloning (depositor) No
Animal _mouse < Mammals
Strain name C3H/He
Gender Unknown
Tissue subcutaneously passaged mouse brain tumors
Primary focus brain
Tumor Brain tumor
SR-RSV-induced brain tumor cells
Classification transformed
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4Y24
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM+10%FBS DMEM+ 10% FBS
Antibiotics 200 U/ml penicillin, 200 μg/ml streptomycin Free
Culture information Passage ratio 1 : 6-16 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Reference information Reference 1
User's Publication 0


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Reference
923  T Kumanishi  Brain tumors induced with Rous sarcoma virus, Schmidt-Ruppin strain. I. Induction of brain tumors in adult mice with Rous chicken sarcoma cells  Jpn J Exp Med  1967  37(5):461-74.  PubMed ID: 4301953  

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