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Cell No. : Cell Name
RCB4548 : PT-r  update : 2022/05/30
CommentRat cell line derived from parathyroid gland.
Comment from the depositor
Terms and conditions1) The availability of the BIOLOGICAL RESOUSE is limited to a RECIPIENT of a non-for-profit organization for a not-for-profit research. 2) Distribution of the BIOLOGICAL RESOUCE to a third party without permission is prohibited. 3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Proc Natl Acad Sci USA 1987;84:3269-3273, J Biol Chem 1989;264:15714-15720, J Biol Chem 1990;265:13661-13668, J Biol Chem 1992;267:24554-24562, Life Sci 2008;82:677-683, Bone 2010;47:534-541) . 5) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sakaguchi, Kazushige
Originator Sakaguchi, Kazushige
Year of deposit 2013
Animal rat < Mammals
Genus Rattus
Species norvegicus
Gender Male
Age at sampling 3 months
Tissue parathyroid gland
Classification normal
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_HF63
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Keratinocyte-SFM + 10mM HEPES
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA), added Keratinocyte-SFM+10%FBS, washed twice with medium
Culture information Passage cell No 2 x 10^(5) cells/100 mm dish
SC frequency Subculture : 2 times/week (not confluent)
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium CELLBANKER 2
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 10
User's Publication 0


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Reference
4569  Kawahara M, Iwasaki Y, Sakaguchi K, Taguchi T, Nishiyama M, Nigawara T, Kambayashi M, Sawada T, Jing X, Miyajima M, Terada Y, Hashimoto K, Suda T.  Involvement of GCMB in the transcriptional regulation of the human parathyroid hormone gene in a parathyroid-derived cell line PT-r: effects of calcium and 1,25(OH)2D3.  Bone  2010  47:534-41  PubMed ID: 20558332   DOI: 10.1016/j.bone.2010.05.031
3654  Kawahara M, Iwasaki Y, Sakaguchi K, Taguchi T, Nishiyama M, Nigawara T, Tsugita M, Kambayashi M, Suda T, Hashimoto K.  Predominant role of 25OHD in the negative regulation of PTH expression: clinical relevance for hypovitaminosis D.  Life Sci  2008  82(13-14):677-83  PubMed ID: 18304585   DOI: 10.1016/j.lfs.2007.12.027
3659  Fujii Y, Tomić M, Stojilković SS, Iida T, Brandi ML, Ogino Y, Sakaguchi K.  Effects of endothelin-1 on Ca2+ signaling and secretion in parathyroid cells.  J Bone Miner Res  1995  10(5):716-25  PubMed ID: 7639107   DOI: 10.1002/jbmr.5650100508
3658  Takagi Y, Shrivastav S, Miki T, Sakaguchi K.  Molecular cloning and expression of the acidic fibroblast growth factor receptors in a rat parathyroid cell line (PT-r). Parathyroid cell-specific calcium-dependent change of ligand accessibility and covalent attachment of heparan sulfate glycosaminoglycan to the receptors.  J Biol Chem  1994  269(38):23743-9  PubMed ID: 8089146  
3653  Sakaguchi K.  Acidic fibroblast growth factor autocrine system as a mediator of calcium-regulated parathyroid cell growth.  J Biol Chem  1992  267(34):24554-62  PubMed ID: 1280262  
3657  Sakaguchi K, Yanagishita M, Takeuchi Y, Aurbach GD.  Identification of heparan sulfate proteoglycan as a high affinity receptor for acidic fibroblast growth factor (aFGF) in a parathyroid cell line.  J Biol Chem  1991  266(11):7270-8  PubMed ID: 1707883  
3652  Takeuchi Y, Sakaguchi K, Yanagishita M, Aurbach GD, Hascall VC.  Extracellular calcium regulates distribution and transport of heparan sulfate proteoglycans in a rat parathyroid cell line.  J Biol Chem  1990  265(23):13661-8  PubMed ID: 2143187  
3656  Sakaguchi K, Ikeda K, Curcio F, Aurbach GD, Brandi ML.  Subclones of a rat parathyroid cell line (PT-r): regulation of growth and production of parathyroid hormone-related peptide (PTHRP).  J Bone Miner Res  1990  5(8):863-9  PubMed ID: 1700579   DOI: 10.1002/jbmr.5650050810
3651  Yanagishita M, Brandi ML, Sakaguchi K.  Characterization of proteoglycans synthesized by a rat parathyroid cell line.  J Biol Chem  1989  264(26):15714-20  PubMed ID: 2768284  
4568  Sakaguchi K, Santora A, Zimering M, Curcio F, Aurbach GD, Brandi ML.  Functional epithelial cell line cloned from rat parathyroid glands.  Proc Natl Acad Sci U S A  1987  84:3269-73  PubMed ID: 3472208   DOI: 10.1073/pnas.84.10.3269

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