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Cell No. : Cell Name
RCB4519 : Orai2^(-/-)DT40 update : 2024/11/13 |
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| Comment | A subline of avian cell line, DT40, lacking Orai2 gene. | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Cell Biol. 2010;190:391-405).2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Kurosaki, Tomohiro | ||
| Originator | Baba, Yoshihiro | |||
| Year of deposit | 2012 | |||
| Original cell | DT40 | |||
| Animal | _avian < Birds | |||
| Tissue | B cell | |||
| Classification | mutant | |||
| Recombinant | recombinant | |||
| Exogene | Bsr, Bleo | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_1T27 | |||
| Medium | Medium List | Culture type | Suspension cells | |
| Culture method | 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol | |||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage cell No | 1.5-2 x 10 5 cells/ml |
| Passage ratio | 1 : 20 split | |||
| SC frequency | Subculture : every 2 days | |||
| Temperature | 39.5 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | 90% FBS + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| Others | Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day. | |||
| Images | ||||
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| Reference information | Reference | 1 | ||
| User's Publication | 0 | |||
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