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Cell No. : Cell Name
RCB4519 : Orai2^(-/-)DT40  update : 2021/05/20
CommentA subline of avian cell line, DT40, lacking Orai2 gene.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Cell Biol. 2010;190:391-405).2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kurosaki, Tomohiro
Originator Baba, Yoshihiro
Year of deposit 2012
Original cell DT40
Animal avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Exogene Bsr, Bleo
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1T27
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10^(5) cells/ml
Passage ratio 1 : 20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10^(7) cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3650  Hawkins BJ1, Irrinki KM, Mallilankaraman K, Lien YC, Wang Y, Bhanumathy CD, Subbiah R, Ritchie MF, Soboloff J, Baba Y, Kurosaki T, Joseph SK, Gill DL, Madesh M.  S-glutathionylation activates STIM1 and alters mitochondrial homeostasis.  J Cell Biol  2010  190(3):391-405  PubMed ID: 20679432   DOI: 10.1083/jcb.201004152

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