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Cell No. : Cell Name
RCB4499 : Onda 10  update : 2022/05/30
CommentHuman cell line derived from anaplastic glioma.
Comment from the depositor
Terms and conditions1) The RECIPIENT must contact the DEVELOPER in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Acta Neuropathol 1988;76:433-40) designated by the DEPOSITOR is required.
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kumanishi, Toshiro
Originator Onda, Kiyoshi
Year of deposit 2012
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 62 years
Tissue temporal lobe, left
Disease name anaplastic glioma
Classification cancer
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3075
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 0

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3647  Onda K, Tanaka R, Washiyama K, Takeda N, Kumanishi T.  Correlation of DNA ploidy and morphological features of human glioma cell cultures with the establishment of cell lines.  Acta Neuropathol  1988  76(5):433-40  PubMed ID: 3188836   DOI: 10.1007/bf00686381

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