Cell No. : Cell Name
RCB4497 : BBLG-50-1
update : 2024/11/11
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| Comment | |
| Comment from the depositor | A reporter cell line derived from the BB13 cell line for identification of proteins that are translocated into or out of the nucleus in response to an extracellular stimulus and other changes in cell status using the inducible translocation trap (ITT) technology. The cell line can also be used for screening of compounds affecting inducible nuclear translocation and nuclear export by ITT. BB13 is derived from a mouse hematopoietic cell line, Ba/F3, and ectopically expresses epidermal growth factor receptor (EGFR) and the anti-apoptotic Bcl-2 protein. BB13 can survive in the absence of IL-3 if EGF is present. The green fluorescent protein (GFP) gene is used as reporter gene. |
| Terms and conditions | 1) No restriction on academic use by nin-profit organizations. 2) All use by profit organizations and commercial use by non-profit organizations. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Cell. 200415:153-9) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
FUJII, Hodaka
|
| Originator |
FUJII, Hodaka
|
| Year of deposit |
2012
|
| Another name |
BBLG
|
| Original cell |
BB13
|
| Cloning (depositor) |
Yes
|
| _Method (depositor) |
limiting dilution
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H
|
| Genus |
Mus
|
| Species |
musculus
|
| Tissue |
bone marrow
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
LexA-d1EGFP : LexA-d1EGFP reporter gene contains eight copies of LexA binding elements, mouse IFNβ minimal promoter, and d1EGFP, human epidermal growth factor (EGF) receptor gene, human bcl-2 gene
|
|
4.2K
base
|
| Selection |
puromycin (0.75 μg/ml), G418 (2mg/ml), Hygromycin (1mg/ml)
|
| Recombinant virus |
No
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Suspension cells
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
RPMI1640, 10% Fetal Calf Serum, 10mM Heaps (pH7.2), 1 x non-essential amino acid, 1mM sodium pyruvate, 5μM 2-mercaptoethanol, 1ng/ml mouse interleukin-3 (IL-3)
|
RPMI1640 + 10% FBS + 10mM Heaps (pH7.2) + 0.1mM NEAA + 1mM sodium pyruvate + 5μM 2-mercaptoethanol + 1ng/ml mouse interleukin-3 (IL-3)
|
| Antibiotics |
200 IU/ml Penicillin, 200 IU/ml Streptomycin
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage cell No |
1x10 5 cell/ml
|
|
| Passage ratio |
|
1 : 10-20 split
|
| SC frequency |
|
Subculture : 3 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Mycoplasma |
(-)
|
|
| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Saturation density |
4x10 5 cell/ml Number/Plate
|
|
| Doubling time |
12
hr
|
|
| Reference information |
Reference |
1
|
| User's Publication |
0
|
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