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Cell No. : Cell Name
RCB4497 : BBLG-50-1  update : 2022/03/28
Comment
Comment from the depositorA reporter cell line derived from the BB13 cell line for identification of proteins that are translocated into or out of the nucleus in response to an extracellular stimulus and other changes in cell status using the inducible translocation trap (ITT) technology. The cell line can also be used for screening of compounds affecting inducible nuclear translocation and nuclear export by ITT. BB13 is derived from a mouse hematopoietic cell line, Ba/F3, and ectopically expresses epidermal growth factor receptor (EGFR) and the anti-apoptotic Bcl-2 protein. BB13 can survive in the absence of IL-3 if EGF is present. The green fluorescent protein (GFP) gene is used as reporter gene.
Terms and conditions1) The RECIPIENT should read Announcement of GFP bioresources 2) No restriction on academic use by nin-profit organizations. 3) All use by profit organizations and commercial use by non-profit organizations. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Cell. 200415:153-9) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor FUJII, Hodaka
Originator FUJII, Hodaka
Year of deposit 2012
Another name BBLG
Original cell BB13
Cloning (depositor) Yes
_Method (depositor) limiting dilution
Animal mouse < Mammals
Strain name C3H
Tissue bone marrow
Classification transformed
Recombinant recombinant
Exogene LexA-d1EGFP : LexA-d1EGFP reporter gene contains eight copies of LexA binding elements, mouse IFNβ minimal promoter, and d1EGFP, human epidermal growth factor (EGF) receptor gene, human bcl-2 gene
4.2K base
puromycin (0.75 μLg/ml), G418 (2mg/ml), Hygromycin (1mg/ml)
Recombinant virus No
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Medium and additives RPMI1640, 10% Fetal Calf Serum, 10mM Heaps (pH7.2), 1 x non-essential amino acid, 1mM sodium pyruvate, 5μM 2-mercaptoethanol, 1ng/ml mouse interleukin-3 (IL-3) RPMI1640 + 10% FBS + 10mM Heaps (pH7.2) + 0.1mM NEAA + 1mM sodium pyruvate + 5μM 2-mercaptoethanol + 1ng/ml mouse interleukin-3 (IL-3)
Antibiotics 200 IU/ml Penicillin, 200 IU/ml Streptomycin Free
Passage method dilution
Culture information Passage cell No 1x10^(5) cell/ml
Passage ratio 1 : 8-10 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Saturation density 4x10^(5) cell/ml Number/Plate
Doubling time 12 hr
Reference information Reference 1
User's Publication 0


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Reference
7463  Hoshino A, Matsumura S, Kondo K, Hirst JA, Fujii H.  Inducible translocation trap: a system for detecting inducible nuclear translocation.  Mol Cell  2004  15(1):153-9  PubMed ID: 15225556   DOI: 10.1016/j.molcel.2004.06.017

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