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Cell No. : Cell Name
RCB4495 : BL2-5-8  update : 2022/03/28
Comment
Comment from the depositorA reporter cell line derived from a mouse hematopoietic cell line, Ba/F3, for identification of proteins that are translocated into or out of the nucleus in response to an extracellular stimulus and other changes in cell status using the inducible translocation trap (ITT) technology. The cell line can also be used for screening of compounds affecting inducible nuclear translocation and nuclear export by ITT. The human CD2 (hCD2) gene is used as reporter gene.
Terms and conditions1) No restriction on academic use by nin-profit organizations. 2) All use by profit organizations and commercial use by non-profit organizations. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Cell. 200415:153-9) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor FUJII, Hodaka
Originator FUJII, Hodaka
Year of deposit 2012
Another name BL2
Original cell Ba/F3
Cloning (depositor) Yes
_Method (depositor) limiting dilution
Animal mouse < Mammals
Strain name C3H
Tissue bone marrow
Classification transformed
Recombinant recombinant
Exogene LexA-hCD2: LexA-hCD2 reporter gene contains eight copies of LexA binding elements, a minimal promoter from mouse IFNβ gene, and the full-length cDNA of hCD2.
4.4K base
Hygromycin (0.75mg/ml)
Recombinant virus No
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_A8X0
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Medium and additives RPMI1640, 10% Fetal Calf Serum, 10mM Heaps (pH7.2), 1 x non-essential amino acid, 1mM sodium pyruvate, 5μM 2-mercaptoethanol, 1ng/ml mouse interleukin-3 (IL-3) RPMI1640 + 10% FBS + 10mM Heaps (pH7.2) + 0.1mM NEAA + 1mM sodium pyruvate + 5μM 2-mercaptoethanol + 1ng/ml mouse interleukin-3 (IL-3)
Antibiotics 200 IU/ml Penicillin, 200 IU/ml Streptomycin Free
Passage method dilution
Culture information Passage cell No 1x10^(5) cell/ml
Passage ratio 1 : 8-10 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-) (-)
Animal PCR OK
Saturation density 4x10^(5) cell/ml
Doubling time 12 hr
Reference information Reference 1
User's Publication 0


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Reference
7463  Hoshino A, Matsumura S, Kondo K, Hirst JA, Fujii H.  Inducible translocation trap: a system for detecting inducible nuclear translocation.  Mol Cell  2004  15(1):153-9  PubMed ID: 15225556

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