Cell No. : Cell Name
RCB4489 : MB86
update : 2024/01/17
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| Comment | Hybridoma cell line producing anti-mouse IgM(b)/Igh6b antibody. |
| Comment from the depositor | |
| Terms and conditions | 1) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purpose only. (Commercial use is prohibited by the DEPOSITOR.) 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Immunogenetics 1986;23:137-139) is necessary. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Nishikawa, Shin-ichi
|
| Originator |
Nishikawa, Shin-ichi
|
| Year of deposit |
2012
|
| Another name |
anti mouse IgM^b
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
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|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_J274
|
|
hybridoma
|
Fusion_cell_1 name |
spleen cells
|
| Fusion_cell_1 strain |
mouse, BALB/c
|
| Fusion_cell_2 name |
X63-Ag8/653
|
| Fusion_cell_2 strain |
mouse myeloma cell line
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|
Fusion method |
PEG #4000, HAT selection
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|
Antibody name |
MB86
|
| Subclass |
IgG
|
| Immunogen |
mouse C57/BL6 spleen cells
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|
Antigen_character |
Igh6b
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
|
RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
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| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
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Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
90% FBS + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgG1
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
0
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| Reference |
|
8941
R Sitia, M Neuberger, C Alberini, P Bet, A Fra, C Valetti, G Williams, C Milstein
Developmental Regulation of IgM Secretion: The Role of the Carboxy-Terminal Cysteine
Cell
1990
60 (5), 781-90
PubMed ID: 2107027
DOI: 10.1016/0092-8674(90)90092-s
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5515
Nishikawa S, Sasaki Y, Kina T, Amagai T, Katsura Y.
A monoclonal antibody against Igh6-4 determinant.
Immunogenetics
1986
23(2):137-9
PubMed ID: 2420716
DOI: 10.1007/bf00377976
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