Back Back
Cell No. : Cell Name
RCB4486 : AFS98  update : 2021/12/21
CommentHybridoma cell line producing anti-mouse c-fms (CD115) antibody.
Comment from the depositor
Terms and conditions1) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purpose only. (Commercial use is prohibited by the DEPOSITOR.) 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Oncogene 1995;11:2469-2476) is necessary.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nishikawa, Shin-ichi
Originator Nishikawa, Satomi
Year of deposit 2012
Another name anti mouse c-fms
Animal mouse x rat < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_KA99
hybridoma Fusion_cell_1 name spleen cells
Fusion_cell_1 strain rat, Wistar
Fusion_cell_2 name X63-Ag8/653
Fusion_cell_2 strain mouse
Fusion method PEG #4000, HAT selection
Antibody name AFS98
Subclass rat IgG2a
Immunogen Murine c-fms fused to hIgG FC
Antigen_name c-fms (CD115)
Antigen_m.w. 150kDa
Antigen_character transmembrane tyrosin-kinase receptor
Antigen_positive monoocyte, osteoclast, macrophage, peritorial exudate cells, plasmacytoid and conventional dendritic cells
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass rat IgG2a
deposit info
lot info
Reference information Reference 21
User's Publication 0

To topTop
8915  Alexandra M Ochsenbein, Sinem Karaman, Steven T Proulx, Rhea Goldmann, Jyothi Chittazhathu, Athanasia Dasargyri, Chloé Chong, Jean-Christophe Leroux, E Richard Stanley, Michael Detmar  Regulation of Lymphangiogenesis in the Diaphragm by Macrophages and VEGFR-3 Signaling  Angiogenesis  2016  19 (4), 513-24  PubMed ID: 27464987   DOI: 10.1007/s10456-016-9523-8
8936  Cynthia Louis, Andrew D Cook, Derek Lacey, Andrew J Fleetwood, Ross Vlahos, Gary P Anderson, John A Hamilton  Specific Contributions of CSF-1 and GM-CSF to the Dynamics of the Mononuclear Phagocyte System  J Immunol  2015  195 (1), 134-44  PubMed ID: 26019271   DOI: 10.4049/jimmunol.1500369
8432  Laetitia Fend, Nathalie Accart, Jacqueline Kintz, Sandrine Cochin, Carine Reymann, Fabrice Le Pogam, Jean-Baptiste Marchand, Thierry Menguy, Philippe Slos, Ronald Rooke, Sylvie Fournel, Jean-Yves Bonnefoy, Xavier Préville, Hélène Haegel  Therapeutic Effects of anti-CD115 Monoclonal Antibody in Mouse Cancer Models Through Dual Inhibition of Tumor-Associated Macrophages and Osteoclasts  PLoS One  2013  8 (9), e73310 2013  PubMed ID: 24019914   DOI: 10.1371/journal.pone.0073310
8919  Melanie Greter, Iva Lelios, Pawel Pelczar, Guillaume Hoeffel, Jeremy Price, Marylene Leboeuf, Thomas M Kündig, Karl Frei, Florent Ginhoux, Miriam Merad, Burkhard Becher  Stroma-derived interleukin-34 Controls the Development and Maintenance of Langerhans Cells and the Maintenance of Microglia  Immunity  2012  37 (6), 1050-1060  PubMed ID: 23177320   DOI: 10.1016/j.immuni.2012.11.001
8924  Takahiro Tsuchiya, Norifumi Nakao, Shigeo Yamamoto, Yoshikazu Hirai, Katsushiro Miyamoto, Hiroshi Tsujibo  NK1.1(+) Cells Regulate Neutrophil Migration in Mice With Acinetobacter Baumannii Pneumonia  Microbiol Immunol  2012  56 (2), 107-16  PubMed ID: 22145920   DOI: 10.1111/j.1348-0421.2011.00402.x
8923  Suwen Wei, Sayan Nandi, Violeta Chitu, Yee-Guide Yeung, Wenfeng Yu, Minmei Huang, Lewis T Williams, Haishan Lin, E Richard Stanley  Functional Overlap but Differential Expression of CSF-1 and IL-34 in Their CSF-1 Receptor-Mediated Regulation of Myeloid Cells  J Leukoc Biol  2010  88 (3), 495-505  PubMed ID: 20504948   DOI: 10.1189/jlb.1209822
8927  Dmitry A Ovchinnikov, Claire E E DeBats, David P Sester, Matthew J Sweet, David A Hume  A Conserved Distal Segment of the Mouse CSF-1 Receptor Promoter Is Required for Maximal Expression of a Reporter Gene in Macrophages and Osteoclasts of Transgenic Mice  J Leukoc Biol  2010  87 (5), 815-22  PubMed ID: 20123678   DOI: 10.1189/jlb.0809557
8580  Masashi Segawa, So-ichiro Fukada, Yukiko Yamamoto, Hiroshi Yahagi, Masanori Kanematsu, Masaki Sato, Takahito Ito, Akiyoshi Uezumi, Shin'ichi Hayashi, Yuko Miyagoe-Suzuki, Shin'ichi Takeda, Kazutake Tsujikawa, Hiroshi Yamamoto  Suppression of Macrophage Functions Impairs Skeletal Muscle Regeneration With Severe Fibrosis  Exp Cell Res  2008  314 (17), 3232-44  PubMed ID: 18775697   DOI: 10.1016/j.yexcr.2008.08.008
8925  Wenfeng Yu, Jian Chen, Ying Xiong, Fiona J Pixley, Xu-Ming Dai, Yee-Guide Yeung, E Richard Stanley  CSF-1 Receptor Structure/Function in MacCsf1r-/- Macrophages: Regulation of Proliferation, Differentiation, and Morphology  J Leukoc Biol  2008  84 (3), 852-63  PubMed ID: 18519746   DOI: 10.1189/jlb.0308171
8931  Ivana Boban, Claire Jacquin, Katie Prior, Tatjana Barisic-Dujmovic, Peter Maye, Stephen H Clark, Hector L Aguila  The 3.6 Kb DNA Fragment From the Rat Col1a1 Gene Promoter Drives the Expression of Genes in Both Osteoblast and Osteoclast Lineage Cells  Bone  2006  39 (6), 1302-12  PubMed ID: 16938497   DOI: 10.1016/j.bone.2006.06.025
8932  Claire Jacquin, Diane E Gran, Sun Kyeong Lee, Joseph A Lorenzo, Hector L Aguila  Identification of Multiple Osteoclast Precursor Populations in Murine Bone Marrow  J Bone Miner Res  2006  21 (1), 67-77  PubMed ID: 16355275   DOI: 10.1359/JBMR.051007
8585  Motokazu Tsuneto, Hidetoshi Yamazaki, Miya Yoshino, Takayuki Yamada, Shin-Ichi Hayashi  Ascorbic Acid Promotes Osteoclastogenesis From Embryonic Stem Cells  Biochem Biophys Res Commun  2005  335 (4), 1239-46  PubMed ID: 16112648   DOI: 10.1016/j.bbrc.2005.08.016
8934  Kiyoshi Kanazawa, Akira Kudo  TRAF2 Is Essential for TNF-alpha-induced Osteoclastogenesis  J Bone Miner Res  2005  20 (5), 840-7  PubMed ID: 15824857   DOI: 10.1359/JBMR.041225
8937  Tatjana Nikolic, Gerben Bouma, Hemmo A Drexhage, Pieter J M Leenen  Diabetes-prone NOD Mice Show an Expanded Subpopulation of Mature Circulating Monocytes, Which Preferentially Develop Into Macrophage-Like Cells in Vitro  J Leukoc Biol  2005  78 (1), 70-9  PubMed ID: 15788439   DOI: 10.1189/jlb.1104662
8939  Michael B Jordan, David Hildeman, John Kappler, Philippa Marrack  An Animal Model of Hemophagocytic Lymphohistiocytosis (HLH): CD8+ T Cells and Interferon Gamma Are Essential for the Disorder  Blood  2004  104 (3), 735-43  PubMed ID: 15069016   DOI: 10.1182/blood-2003-10-3413
8910  Peter Neumeister, Fiona J Pixley, Ying Xiong, Huafeng Xie, Kongming Wu, Anthony Ashton, Michael Cammer, Amanda Chan, Marc Symons, E Richard Stanley, Richard G Pestell  Cyclin D1 Governs Adhesion and Motility of Macrophages  Mol Biol Cell  2003  14 (5), 2005-15  PubMed ID: 12802071   DOI: 10.1091/mbc.02-07-0102
8926  Y H Yang, J A Hamilton  Dependence of interleukin-1-induced Arthritis on Granulocyte-Macrophage Colony-Stimulating Factor  Arthritis Rheum  2001  44 (1), 111-9  PubMed ID: 11212148   DOI: 10.1002/1529-0131(200101)44:1<111::AID-ANR15>3.0.CO;2-1
8930  K Kaji, R Katogi, Y Azuma, A Naito, J I Inoue, A Kudo  Tumor Necrosis Factor Alpha-Induced Osteoclastogenesis Requires Tumor Necrosis Factor Receptor-Associated Factor 6  J Bone Miner Res  2001  16 (9), 1593-9  PubMed ID: 11547829   DOI: 10.1359/jbmr.2001.16.9.1593
8908  T Yamane, T Kunisada, H Yamazaki, T Nakano, S H Orkin, S I Hayashi  Sequential Requirements for SCL/tal-1, GATA-2, Macrophage Colony-Stimulating Factor, and Osteoclast Differentiation Factor/Osteoprotegerin Ligand in Osteoclast Development  Exp Hematol  2000  28 (7), 833-40  PubMed ID: 10907645   DOI: 10.1016/s0301-472x(00)00175-2
8928  J A Hamilton, R Byrne, G Whitty  Particulate Adjuvants Can Induce Macrophage Survival, DNA Synthesis, and a Synergistic Proliferative Response to GM-CSF and CSF-1  J Leukoc Biol  2000  67 (2), 226-32  PubMed ID: 10670584  
5512  Sudo T, Nishikawa S, Ogawa M, Kataoka H, Ohno N, Izawa A, Hayashi S, Nishikawa S.  Functional hierarchy of c-kit and c-fms in intramarrow production of CFU-M.  Oncogene  1995  11(12):2469-76  PubMed ID: 8545103  

To topTop
User's Publication

Back Back Return Top Page