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Cell No. : Cell Name
RCB4485 : A2F10  update : 2021/05/20
CommentHybridoma cell line producing anti-mouse Flk-2/Flt-3 (CD135) antibody.
Comment from the depositor
Terms and conditions1) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purpose only. (Commercial use is prohibited by the DEPOSITOR.) 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Exp Hematol 1998;26:478-488) is necessary.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishikawa, Shin-ichi
Originator Ogawa, Minetaro
Year of deposit 2012
Another name anti mouse Flk-2/Flk-3
Animal mouse x rat < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_KA98
hybridoma Fusion_cell_1 name spleen cells
Fusion_cell_1 strain rat, Wistar
Fusion_cell_2 name X63-Ag8/653
Fusion_cell_2 strain mouse
Fusion method PEG #4000, HAT selection
Antibody name A2F10
Subclass rat IgG2a
Immunogen Rat myeloma cell line Y3 which the murine flt-3 gene is introduced.
Antigen_name Flk2/Flt3
Antigen_m.w. 150kDa
Antigen_character transmembrane tyrosin-kinase receptor
Antigen_positive early hematopoietic progenitor
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass rat IgG2a
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
5511  Ogawa M, Sugawara S, Kunisada T, Sudo T, Hayashi S, Nishikawa S, Kodama H, Nishikawa S.  Flt3/Flk-2 and c-Kit are not essential for the proliferation of B lymphoid progenitor cells in the bone marrow of the adult mouse.  Exp Hematol  1998  26(6):478-88  PubMed ID: 9620281  

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