Cell No. : Cell Name
RCB4483 : APB5
update : 2025/01/16
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| Comment | Hybridoma cell line producing anti-mouse PDGF Receptor beta (CD140b) antibody. |
| Comment from the depositor | |
| Terms and conditions | 1) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purpose only. (Commercial use is prohibited by the DEPOSITOR.) 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Circulation 2001;103:2955-2960) is necessary. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Nishikawa, Shin-ichi
|
| Originator |
Nishikawa, Satomi
|
| Year of deposit |
2012
|
| Another name |
anti mouse PDGFRb
|
| Animal |
_mouse x rat
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
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|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_KB08
|
|
hybridoma
|
Fusion_cell_1 name |
spleen cells
|
| Fusion_cell_1 strain |
rat, Wistar
|
| Fusion_cell_2 name |
X63-Ag8/653
|
| Fusion_cell_2 strain |
mouse
|
|
Fusion method |
PEG #4000, HAT selection
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|
Antibody name |
APB5
|
| Subclass |
rat IgG2a
|
| Immunogen |
Murine PDGFRb fused to hIgG FC
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|
Antigen_name |
CD140b (PDGFRb)
|
| Antigen_m.w. |
190kDa
|
| Antigen_character |
transmembrane tyrosin-kinase receptor
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|
Antigen_positive |
various cells
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
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| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
90% FBS + 10% DMSO
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| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
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| Antibody subclass |
|
rat IgG2a
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
0
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| Reference |
|
8593
Masami Kishi, Yoshinori Aono, Seidai Sato, Kazuya Koyama, Momoyo Azuma, Shuichi Abe, Hiroshi Kawano, Jun Kishi, Yuko Toyoda, Hiroyasu Okazaki, Hirohisa Ogawa, Hisanori Uehara, Yasuhiko Nishioka
Blockade of Platelet-Derived Growth Factor Receptor-β, Not Receptor-α Ameliorates Bleomycin-Induced Pulmonary Fibrosis in Mice
PLoS One
2018
13 (12), e0209786
PubMed ID: 30596712
DOI: 10.1371/journal.pone.0209786
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5509
Sano H, Sudo T, Yokode M, Murayama T, Kataoka H, Takakura N, Nishikawa S, Nishikawa SI, Kita T.
Functional blockade of platelet-derived growth factor receptor-beta but not of receptor-alpha prevents vascular smooth muscle cell accumulation in fibrous cap lesions in apolipoprotein E-deficient mice.
Circulation
2001
103(24):2955-60
PubMed ID: 11413086
DOI: 10.1161/01.cir.103.24.2955
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5506
Takakura N, Yoshida H, Kunisada T, Nishikawa S, Nishikawa SI.
Involvement of platelet-derived growth factor receptor-alpha in hair canal formation.
J Invest Dermatol
1996
107(5):770-7
PubMed ID: 8875964
DOI: 10.1111/1523-1747.ep12371802
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