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Cell No. : Cell Name
RCB4482 : APA5  update : 2021/05/20
CommentHybridoma cell line producing anti-mouse PDGF Receptor alpha (CD140a) antibody.
Comment from the depositor
Terms and conditions1) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purpose only. (Commercial use is prohibited by the DEPOSITOR.) 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Invest Dermatol 1996;107:770-777) is necessary.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishikawa, Shin-ichi
Originator Takakura, Nobuyuki
Year of deposit 2012
Another name anti mouse PDGFRa
Animal mouse x rat < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_KB07
hybridoma Fusion_cell_1 name spleen cells
Fusion_cell_1 strain rat, Wistar
Fusion_cell_1 gender Female
Fusion_cell_2 name X63-Ag8/653
Fusion_cell_2 strain mouse
Fusion method PEG #4000, HAT selection
Antibody name APA5
Subclass rat IgG2a
Immunogen Murine PDGFRa fused to hIgG FC
Antigen_name CD140a (PDGFRa)
Antigen_m.w. 190kDa
Antigen_character transmembrane tyrosin-kinase receptor
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass rat IgG2a
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
8593  Masami Kishi, Yoshinori Aono, Seidai Sato, Kazuya Koyama, Momoyo Azuma, Shuichi Abe, Hiroshi Kawano, Jun Kishi, Yuko Toyoda, Hiroyasu Okazaki, Hirohisa Ogawa, Hisanori Uehara, Yasuhiko Nishioka  Blockade of Platelet-Derived Growth Factor Receptor-β, Not Receptor-α Ameliorates Bleomycin-Induced Pulmonary Fibrosis in Mice  PLoS One  2018  13 (12), e0209786  PubMed ID: 30596712   DOI: 10.1371/journal.pone.0209786
5506  Takakura N, Yoshida H, Kunisada T, Nishikawa S, Nishikawa SI.  Involvement of platelet-derived growth factor receptor-alpha in hair canal formation.  J Invest Dermatol  1996  107(5):770-7  PubMed ID: 8875964   DOI: 10.1111/1523-1747.ep12371802

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