細胞番号 : 細胞名
RCB4481 : A7R34
update : 2024/01/17
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| 細胞特性(Comment:英) | Hybridoma cell line producing anti-mouse IL-7 Receptor alpha (CD127) antibody. |
| 細胞特性(日) | ハイブリドーマ細胞株。抗マウスIL-7受容体アルファ (CD127)抗体産生。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | 1) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purpose only. (Commercial use is prohibited by the DEPOSITOR.) 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Proc Natl Acad Sci USA 1993;90:9125-9129) is necessary. |
| 使用条件(日) | 1) 教育･研究目的のみ利用可。商業目的の利用不可。 2) 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Proc Natl Acad Sci USA 1993;90:9125-9129) を引用すること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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|
寄託者 |
Nishikawa, Shin-ichi
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| 樹立者 |
Nishikawa, Satomi
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| 寄託日 |
2012
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| 別名 |
anti mouse IL-7Ra
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| 動物種 |
_mouse x rat
< Hybrid
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| 採取組織 |
hybridoma
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| 細胞分類 |
hybrid cells
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|
細胞寿命 |
infinite
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| 細胞形態 |
lymphocyte-like
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| Cellosaurus(Expasy) |
CVCL_KB00
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ハイブリドーマ関連
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|
親細胞１の細胞名 |
spleen cells
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| 親細胞１の動物名及び系統名 |
rat, Wistar
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| 親細胞２の細胞名 |
X63-Ag8/653
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| 親細胞２の動物名及び系統名 |
mouse
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|
細胞融合・選択法 |
PEG #4000, HAT selection
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|
単クローン性抗体名 |
A7R34
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| サブクラス |
rat IgG2a
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| 免疫に用いた材料 |
Murine IL-7Ra fused to hIgG FC
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抗体で検出される抗原の名称 |
CD127(IL-7Ra)
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| 分子量 |
65〜75kDa
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| 抗体で検出される抗原の性状 |
transmembrane protein
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陽性細胞・組織 |
lymphocyte
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Suspension cells
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| 培養方法 |
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General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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| 培地 |
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RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
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| 抗生物質 |
|
Free
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継代方法 |
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dilution
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|
継代密度 |
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1 : 10-15 split
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| 継代・培地交換頻度 |
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Subculture : 2 times/week
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| 培養最適温度 |
|
37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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90% FBS + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ/アコレプラズマ |
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(-)
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| 産生抗体のサブクラス |
|
rat IgG2a
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
17件
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| Reference(日) |
0件
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| 利用者成果(英) |
0件
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| 利用者成果(日) |
0件
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| Reference(英) |
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8316
Takaharu Sasaki, Kazuyo Moro, Tetsuya Kubota, Naoto Kubota, Tamotsu Kato, Hiroshi Ohno, Susumu Nakae, Hirohisa Saito, Shigeo Koyasu
Innate Lymphoid Cells in the Induction of Obesity
Cell Rep
2019
28 (1), 202-217
PubMed ID: 31269440
DOI: 10.1016/j.celrep.2019.06.016
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8904
Haruyo Nakajima-Adachi, Akira Kikuchi, Yoko Fujimura, Kyoko Shibahara, Tsuyoshi Makino, Masae Goseki-Sone, Miran Kihara-Fujioka, Tomonori Nochi, Yosuke Kurashima, Osamu Igarashi, Masafumi Yamamoto, Jun Kunisawa, Masako Toda, Shuichi Kaminogawa, Ryuichiro Sato, Hiroshi Kiyono, Satoshi Hachimura
Peyer's Patches and Mesenteric Lymph Nodes Cooperatively Promote Enteropathy in a Mouse Model of Food Allergy
PLoS One
2014
9 (10), e107492
PubMed ID: 25290461
DOI: 10.1371/journal.pone.0107492
|
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8881
Marko Knoll, Szandor Simmons, Corinne Bouquet, Joachim R Grün, Fritz Melchers
miR-221 Redirects Precursor B Cells to the BM and Regulates Their Residence
Eur J Immunol
2013
43 (9), 2497-506
PubMed ID: 23716169
DOI: 10.1002/eji.201343367
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8902
Sandrine Schmutz, Nabil Bosco, Stephane Chappaz, Onur Boyman, Hans Acha-Orbea, Rhodri Ceredig, Antonius G Rolink, Daniela Finke
Cutting Edge: IL-7 Regulates the Peripheral Pool of Adult ROR Gamma+ Lymphoid Tissue Inducer Cells
J Immunol
2009
183 (4), 2217-21
PubMed ID: 19635901
DOI: 10.4049/jimmunol.0802911
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8897
Keiichi Kiriya, Norihiko Watanabe, Akiyoshi Nishio, Kazuichi Okazaki, Masahiro Kido, Kazuyuki Saga, Junya Tanaka, Takuji Akamatsu, Shinya Ohashi, Masanori Asada, Toshiro Fukui, Tsutomu Chiba
Essential Role of Peyer's Patches in the Development of Helicobacter-induced Gastritis
Int Immunol
2007
19 (4), 435-46
PubMed ID: 17314082
DOI: 10.1093/intimm/dxm008
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8905
Vincent Feuillet, Bruno Lucas, James P Di Santo, Georges Bismuth, Alain Trautmann
Multiple Survival Signals Are Delivered by Dendritic Cells to Naive CD4+ T Cells
Eur J Immunol
2005
35 (9), 2563-72
PubMed ID: 16078277
DOI: 10.1002/eji.200526127
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8906
Grzegorz Terszowski, Claudia Waskow, Peter Conradt, Dido Lenze, Jessica Koenigsmann, Dirk Carstanjen, Ivan Horak, Hans-Reimer Rodewald
Prospective Isolation and Global Gene Expression Analysis of the Erythrocyte Colony-Forming Unit (CFU-E)
Blood
2005
105 (5), 1937-45
PubMed ID: 15522951
DOI: 10.1182/blood-2004-09-3459
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8894
L Vivien, C Benoist, D Mathis
T Lymphocytes Need IL-7 but Not IL-4 or IL-6 to Survive in Vivo
Int Immunol
2001
13 (6), 763-8
PubMed ID: 11369703
DOI: 10.1093/intimm/13.6.763
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8899
J Kang, A Volkmann, D H Raulet
Evidence That Gammadelta Versus Alphabeta T Cell Fate Determination Is Initiated Independently of T Cell Receptor Signaling
J Exp Med
2001
193 (6), 689-98
PubMed ID: 11257136
DOI: 10.1084/jem.193.6.689
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8896
F M Smart, A R Venkitaraman
Inhibition of Interleukin 7 Receptor Signaling by Antigen Receptor Assembly
J Exp Med
2000
191 (4), 737-42
PubMed ID: 10684865
DOI: 10.1084/jem.191.4.737
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8900
I Kato, T Miyazaki, T Nakamura, A Kudo
Inducible Differentiation and Apoptosis of the pre-B Cell Receptor-Positive pre-B Cell Line
Int Immunol
2000
12 (3), 325-34
PubMed ID: 10700467
DOI: 10.1093/intimm/12.3.325
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8895
T Miyazaki, I Kato, S Takeshita, H Karasuyama, A Kudo
Lambda5 Is Required for Rearrangement of the Ig Kappa Light Chain Gene in pro-B Cell Lines
Int Immunol
1999
11 (8), 1195-202
PubMed ID: 10421777
DOI: 10.1093/intimm/11.8.1195
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8898
J Tong, H Kishi, T Matsuda, A Muraguchi
A Bone Marrow-Derived Stroma Cell Line, ST2, Can Support the Differentiation of Fetal Thymocytes From the CD4+ CD8+ Double Negative to the CD4+ CD8+ Double Positive Differentiation Stage in Vitro
Immunology
1999
97 (4), 672-8
PubMed ID: 10457222
DOI: 10.1046/j.1365-2567.1999.00822.x
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8753
K Akashi, M Kondo, I L Weissman
Two Distinct Pathways of Positive Selection for Thymocytes
Proc Natl Acad Sci U S A
1998
95 (5), 2486-91
PubMed ID: 9482912
DOI: 10.1073/pnas.95.5.2486
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8901
M Uehira, H Matsuda, A Nakamura, H Nishimoto
Immunologic Abnormalities Exhibited in IL-7 Transgenic Mice With Dermatitis
J Invest Dermatol
1998
110 (5), 740-5
PubMed ID: 9579538
DOI: 10.1046/j.1523-1747.1998.00179.x
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8892
E Maraskovsky 1, M Teepe, P J Morrissey, S Braddy, R E Miller, D H Lynch, J J Peschon
Impaired Survival and Proliferation in IL-7 Receptor-Deficient Peripheral T Cells
J Immunol
1996
157 (12), 5315-23
PubMed ID: 8955178
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5508
Sudo T, Nishikawa S, Ohno N, Akiyama N, Tamakoshi M, Yoshida H, Nishikawa S.
Expression and function of the interleukin 7 receptor in murine lymphocytes.
Proc Natl Acad Sci U S A.
1993
90(19):9125-9
PubMed ID: 8415665
DOI: 10.1073/pnas.90.19.9125
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