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Cell No. : Cell Name
RCB4481 : A7R34  update : 2021/05/20
CommentHybridoma cell line producing anti-mouse IL-7 Receptor alpha (CD127) antibody.
Comment from the depositor
Terms and conditions1) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purpose only. (Commercial use is prohibited by the DEPOSITOR.) 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Proc Natl Acad Sci USA 1993;90:9125-9129) is necessary.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishikawa, Shin-ichi
Originator Nishikawa, Satomi
Year of deposit 2012
Another name anti mouse IL-7Ra
Animal mouse x rat < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_KB00
hybridoma Fusion_cell_1 name spleen cells
Fusion_cell_1 strain rat, Wistar
Fusion_cell_2 name X63-Ag8/653
Fusion_cell_2 strain mouse
Fusion method PEG #4000, HAT selection
Antibody name A7R34
Subclass rat IgG2a
Immunogen Murine IL-7Ra fused to hIgG FC
Antigen_name CD127(IL-7Ra)
Antigen_m.w. 65〜75kDa
Antigen_character transmembrane protein
Antigen_positive lymphocyte
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10-15 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass rat IgG2a
Images
deposit info
lot info
Reference information Reference 16
User's Publication 0


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Reference
8316  Takaharu Sasaki, Kazuyo Moro, Tetsuya Kubota, Naoto Kubota, Tamotsu Kato, Hiroshi Ohno, Susumu Nakae, Hirohisa Saito, Shigeo Koyasu   Innate Lymphoid Cells in the Induction of Obesity  Cell Rep   2019  28 (1), 202-217  PubMed ID: 31269440   DOI: 10.1016/j.celrep.2019.06.016
8904  Haruyo Nakajima-Adachi, Akira Kikuchi, Yoko Fujimura, Kyoko Shibahara, Tsuyoshi Makino, Masae Goseki-Sone, Miran Kihara-Fujioka, Tomonori Nochi, Yosuke Kurashima, Osamu Igarashi, Masafumi Yamamoto, Jun Kunisawa, Masako Toda, Shuichi Kaminogawa, Ryuichiro Sato, Hiroshi Kiyono, Satoshi Hachimura  Peyer's Patches and Mesenteric Lymph Nodes Cooperatively Promote Enteropathy in a Mouse Model of Food Allergy  PLoS One  2014  9 (10), e107492  PubMed ID: 25290461   DOI: 10.1371/journal.pone.0107492
8881  Marko Knoll, Szandor Simmons, Corinne Bouquet, Joachim R Grün, Fritz Melchers  miR-221 Redirects Precursor B Cells to the BM and Regulates Their Residence  Eur J Immunol  2013  43 (9), 2497-506  PubMed ID: 23716169   DOI: 10.1002/eji.201343367
8902  Sandrine Schmutz, Nabil Bosco, Stephane Chappaz, Onur Boyman, Hans Acha-Orbea, Rhodri Ceredig, Antonius G Rolink, Daniela Finke  Cutting Edge: IL-7 Regulates the Peripheral Pool of Adult ROR Gamma+ Lymphoid Tissue Inducer Cells  J Immunol  2009  183 (4), 2217-21  PubMed ID: 19635901   DOI: 10.4049/jimmunol.0802911
8897  Keiichi Kiriya, Norihiko Watanabe, Akiyoshi Nishio, Kazuichi Okazaki, Masahiro Kido, Kazuyuki Saga, Junya Tanaka, Takuji Akamatsu, Shinya Ohashi, Masanori Asada, Toshiro Fukui, Tsutomu Chiba  Essential Role of Peyer's Patches in the Development of Helicobacter-induced Gastritis  Int Immunol  2007  19 (4), 435-46  PubMed ID: 17314082   DOI: 10.1093/intimm/dxm008
8905  Vincent Feuillet, Bruno Lucas, James P Di Santo, Georges Bismuth, Alain Trautmann  Multiple Survival Signals Are Delivered by Dendritic Cells to Naive CD4+ T Cells  Eur J Immunol  2005  35 (9), 2563-72  PubMed ID: 16078277   DOI: 10.1002/eji.200526127
8906  Grzegorz Terszowski, Claudia Waskow, Peter Conradt, Dido Lenze, Jessica Koenigsmann, Dirk Carstanjen, Ivan Horak, Hans-Reimer Rodewald  Prospective Isolation and Global Gene Expression Analysis of the Erythrocyte Colony-Forming Unit (CFU-E)  Blood  2005  105 (5), 1937-45  PubMed ID: 15522951   DOI: 10.1182/blood-2004-09-3459
8899  J Kang, A Volkmann, D H Raulet  Evidence That Gammadelta Versus Alphabeta T Cell Fate Determination Is Initiated Independently of T Cell Receptor Signaling  J Exp Med  2001  193 (6), 689-98  PubMed ID: 11257136   DOI: 10.1084/jem.193.6.689
8896  F M Smart, A R Venkitaraman  Inhibition of Interleukin 7 Receptor Signaling by Antigen Receptor Assembly  J Exp Med  2000  191 (4), 737-42  PubMed ID: 10684865   DOI: 10.1084/jem.191.4.737
8900  I Kato, T Miyazaki, T Nakamura, A Kudo  Inducible Differentiation and Apoptosis of the pre-B Cell Receptor-Positive pre-B Cell Line  Int Immunol  2000  12 (3), 325-34  PubMed ID: 10700467   DOI: 10.1093/intimm/12.3.325
8895  T Miyazaki, I Kato, S Takeshita, H Karasuyama, A Kudo  Lambda5 Is Required for Rearrangement of the Ig Kappa Light Chain Gene in pro-B Cell Lines  Int Immunol  1999  11 (8), 1195-202  PubMed ID: 10421777   DOI: 10.1093/intimm/11.8.1195
8898  J Tong, H Kishi, T Matsuda, A Muraguchi  A Bone Marrow-Derived Stroma Cell Line, ST2, Can Support the Differentiation of Fetal Thymocytes From the CD4+ CD8+ Double Negative to the CD4+ CD8+ Double Positive Differentiation Stage in Vitro  Immunology  1999  97 (4), 672-8  PubMed ID: 10457222   DOI: 10.1046/j.1365-2567.1999.00822.x
8753  K Akashi, M Kondo, I L Weissman  Two Distinct Pathways of Positive Selection for Thymocytes  Proc Natl Acad Sci U S A  1998  95 (5), 2486-91  PubMed ID: 9482912   DOI: 10.1073/pnas.95.5.2486
8901  M Uehira, H Matsuda, A Nakamura, H Nishimoto  Immunologic Abnormalities Exhibited in IL-7 Transgenic Mice With Dermatitis  J Invest Dermatol  1998  110 (5), 740-5  PubMed ID: 9579538   DOI: 10.1046/j.1523-1747.1998.00179.x
8892  E Maraskovsky 1, M Teepe, P J Morrissey, S Braddy, R E Miller, D H Lynch, J J Peschon  Impaired Survival and Proliferation in IL-7 Receptor-Deficient Peripheral T Cells  J Immunol  1996  157 (12), 5315-23  PubMed ID: 8955178  
5508  Sudo T, Nishikawa S, Ohno N, Akiyama N, Tamakoshi M, Yoshida H, Nishikawa S.  Expression and function of the interleukin 7 receptor in murine lymphocytes.  Proc Natl Acad Sci U S A.  1993  90(19):9125-9  PubMed ID: 8415665  

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