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Cell No. : Cell Name
RCB4480 : ACK45  update : 2021/05/20
CommentHybridoma cell line producing anti-mouse c-kit (CD117) antibody.
Comment from the depositor
Terms and conditions1) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purpose only. (Commercial use is prohibited by the DEPOSITOR.) 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (EMBO J 1991;10:2111-2118) is necessary.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishikawa, Shin-ichi
Originator Nishikawa, Satomi
Year of deposit 2012
Another name anti mouse c-kit
Animal mouse x rat < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_KB05
hybridoma Fusion_cell_1 name spleen cells
Fusion_cell_1 strain rat, Wistar
Fusion_cell_2 name X63-Ag8/653
Fusion_cell_2 strain mouse
Fusion method PEG #4000, HAT selection
Antibody name ACK45
Subclass rat IgG2b
Immunogen Murine IL-3 dependent mast cells
Antigen_name CD117(c-kit)
Antigen_m.w. 145kDa
Antigen_character transmembrane tyrosin-kinase receptor
Antigen_positive various cells
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass rat IgG2b
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
5507  Nishikawa S, Kusakabe M, Yoshinaga K, Ogawa M, Hayashi S, Kunisada T, Era T, Sakakura T, Nishikawa S.  In utero manipulation of coat color formation by a monoclonal anti-c-kit antibody: two distinct waves of c-kit-dependency during melanocyte development.  EMBO J  1991  10(8):2111-8  PubMed ID: 1712289

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