細胞番号 : 細胞名
RCB4478 : ACK4
update : 2024/01/17
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| 細胞特性(Comment:英) | Hybridoma cell line producing anti-mouse c-kit (CD117) antibody. |
| 細胞特性(日) | ハイブリドーマ細胞株。抗マウスc-kit (CD117)抗体産生。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | 1) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purpose only. (Commercial use is prohibited by the DEPOSITOR.) 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Exp Med 1991;174:63-71) is necessary. |
| 使用条件(日) | 1) 教育･研究目的のみ利用可。商業目的の利用不可。 2) 利用者は、研究成果の公表にあたって寄託者の指定する文献 (J Exp Med 1991;174:63-71) を引用すること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Nishikawa, Shin-ichi
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| 樹立者 |
Nishikawa, Satomi
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| 寄託日 |
2012
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| 別名 |
anti mouse c-kit
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| 動物種 |
_mouse x rat
< Hybrid
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| 採取組織 |
hybridoma
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| 細胞分類 |
hybrid cells
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|
細胞寿命 |
infinite
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| 細胞形態 |
lymphocyte-like
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| Cellosaurus(Expasy) |
CVCL_KB04
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ハイブリドーマ関連
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親細胞１の細胞名 |
spleen cells
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| 親細胞１の動物名及び系統名 |
rat, Wistar
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| 親細胞２の細胞名 |
X63-Ag8/653
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| 親細胞２の動物名及び系統名 |
mouse
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|
細胞融合・選択法 |
PEG #4000, HAT selection
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|
単クローン性抗体名 |
ACK4
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| サブクラス |
rat IgG2a
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| 免疫に用いた材料 |
Murine IL-3 dependent mast cells
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抗体で検出される抗原の名称 |
CD117(c-kit)
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| 分子量 |
145kDa
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| 抗体で検出される抗原の性状 |
transmembrane tyrosin-kinase receptor
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陽性細胞・組織 |
various cells
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Suspension cells
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| 培養方法 |
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General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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| 培地 |
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RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
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| 抗生物質 |
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Free
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継代方法 |
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dilution
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|
継代密度 |
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1 : 10 split
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| 継代・培地交換頻度 |
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Subculture : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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90% FBS + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ/アコレプラズマ |
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(-)
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| 産生抗体のサブクラス |
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rat IgG2a
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
15件
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| Reference(日) |
0件
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| 利用者成果(英) |
0件
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| 利用者成果(日) |
0件
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| Reference(英) |
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8881
Marko Knoll, Szandor Simmons, Corinne Bouquet, Joachim R Grün, Fritz Melchers
miR-221 Redirects Precursor B Cells to the BM and Regulates Their Residence
Eur J Immunol
2013
43 (9), 2497-506
PubMed ID: 23716169
DOI: 10.1002/eji.201343367
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8826
Hongfang Wang, L Jeanne Pierce, Gerald J Spangrude
Distinct Roles of IL-7 and Stem Cell Factor in the OP9-DL1 T-cell Differentiation Culture System
Exp Hematol
2006
34 (12), 1730-40
PubMed ID: 17157170
DOI: 10.1016/j.exphem.2006.08.001
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8883
D D Spyropoulos, P N Pharr, K R Lavenburg, P Jackers, T S Papas, M Ogawa, D K Watson
Hemorrhage, Impaired Hematopoiesis, and Lethality in Mouse Embryos Carrying a Targeted Disruption of the Fli1 Transcription Factor
Mol Cell Biol
2000
20 (15), 5643-52
PubMed ID: 10891501
DOI: 10.1128/mcb.20.15.5643-5652.2000
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8887
F Tajima, T Sato, J H Laver, M Ogawa
CD34 Expression by Murine Hematopoietic Stem Cells Mobilized by Granulocyte Colony-Stimulating Factor
Blood
2000
96 (5), 1989-93
PubMed ID: 10961905
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8870
F Hirayama, Y Aiba, K Ikebuchi, S Sekiguchi, M Ogawa
Differentiation in Culture of Murine Primitive Lymphohematopoietic Progenitors Toward T-cell Lineage
Blood
1999
93 (12), 4187-95
PubMed ID: 10361116
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8885
T Sato, J H Laver, M Ogawa
Reversible Expression of CD34 by Murine Hematopoietic Stem Cells
Blood
1999
94 (8), 2548-54
PubMed ID: 10515856
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8872
T Matsunaga, T Kato, H Miyazaki, M Ogawa
Thrombopoietin Promotes the Survival of Murine Hematopoietic Long-Term Reconstituting Cells: Comparison With the Effects of FLT3/FLK-2 Ligand and interleukin-6
Blood
1998
92 (2), 452-61
PubMed ID: 9657744
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8873
T Matsunaga, F Hirayama, Y Yonemura, R Murray, M Ogawa
Negative Regulation by interleukin-3 (IL-3) of Mouse Early B-cell Progenitors and Stem Cells in Culture: Transduction of the Negative Signals by Betac and betaIL-3 Proteins of IL-3 Receptor and Absence of Negative Regulation by Granulocyte-Macrophage Colony-Stimulating Factor
Blood
1998
92 (3), 901-7
PubMed ID: 9680358
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8884
I L Mårtensson, F Melchers, T H Winkler
A Transgenic Marker for Mouse B Lymphoid Precursors
J Exp Med
1997
185 (4), 653-61
PubMed ID: 9034144
DOI: 10.1084/jem.185.4.653
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8717
Y Yamamoto, R Yasumizu, Y Amou, N Watanabe, N Nishio, J Toki, S Fukuhara, S Ikehara
Characterization of Peripheral Blood Stem Cells in Mice
Blood
1996
88 (2), 445-54
PubMed ID: 8695791
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8869
D Orlic, L J Girard, C T Jordan, S M Anderson, A P Cline, D M Bodine
The Level of mRNA Encoding the Amphotropic Retrovirus Receptor in Mouse and Human Hematopoietic Stem Cells Is Low and Correlates With the Efficiency of Retrovirus Transduction
Proc Natl Acad Sci U S A
1996
93 (20), 11097-102
PubMed ID: 8855315
DOI: 10.1073/pnas.93.20.11097
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8871
N Nishio, H Hisha, H Ogata, M Inaba, Y Yamamoto, Y Amoh, R Yasumizu, K Hanada, H Hamada, S Ikehara
Changes in Markers, Receptors and Adhesion Molecules Expressed on Murine Hemopoietic Stem Cells After a Single Injection of 5-fluorouracil
Stem Cells
1996
14 (5), 584-91
PubMed ID: 8888499
DOI: 10.1002/stem.140584
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8868
D Löffert , S Schaal, A Ehlich, R R Hardy, Y R Zou, W Müller, K Rajewsky
Early B-cell Development in the Mouse: Insights From Mutations Introduced by Gene Targeting
Immunol Rev
1994
137, 135-53
PubMed ID: 8034332
DOI: 10.1111/j.1600-065x.1994.tb00662.x
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8876
D M Bodine, N E Seidel, M S Gale, A W Nienhuis, D Orlic
Efficient Retrovirus Transduction of Mouse Pluripotent Hematopoietic Stem Cells Mobilized Into the Peripheral Blood by Treatment With Granulocyte Colony-Stimulating Factor and Stem Cell Factor
Blood
1994
84 (5), 1482-91
PubMed ID: 7520774
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3645
Ogawa M, Matsuzaki Y, Nishikawa S, Hayashi S, Kunisada T, Sudo T, Kina T, Nakauchi H, Nishikawa S.
Expression and function of c-kit in hemopoietic progenitor cells.
J Exp Med
1991
174(1):63-71
PubMed ID: 1711568
DOI: 10.1084/jem.174.1.63
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