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Cell No. : Cell Name
RCB4478 : ACK4  update : 2021/05/20
CommentHybridoma cell line producing anti-mouse c-kit (CD117) antibody.
Comment from the depositor
Terms and conditions1) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purpose only. (Commercial use is prohibited by the DEPOSITOR.) 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Exp Med 1991;174:63-71) is necessary.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nishikawa, Shin-ichi
Originator Nishikawa, Satomi
Year of deposit 2012
Another name anti mouse c-kit
Animal mouse x rat < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_KB04
hybridoma Fusion_cell_1 name spleen cells
Fusion_cell_1 strain rat, Wistar
Fusion_cell_2 name X63-Ag8/653
Fusion_cell_2 strain mouse
Fusion method PEG #4000, HAT selection
Antibody name ACK4
Subclass rat IgG2a
Immunogen Murine IL-3 dependent mast cells
Antigen_name CD117(c-kit)
Antigen_m.w. 145kDa
Antigen_character transmembrane tyrosin-kinase receptor
Antigen_positive various cells
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass rat IgG2a
deposit info
lot info
Reference information Reference 15
User's Publication 0

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8881  Marko Knoll, Szandor Simmons, Corinne Bouquet, Joachim R Grün, Fritz Melchers  miR-221 Redirects Precursor B Cells to the BM and Regulates Their Residence  Eur J Immunol  2013  43 (9), 2497-506  PubMed ID: 23716169   DOI: 10.1002/eji.201343367
8826  Hongfang Wang, L Jeanne Pierce, Gerald J Spangrude  Distinct Roles of IL-7 and Stem Cell Factor in the OP9-DL1 T-cell Differentiation Culture System  Exp Hematol  2006  34 (12), 1730-40  PubMed ID: 17157170   DOI: 10.1016/j.exphem.2006.08.001
8883  D D Spyropoulos, P N Pharr, K R Lavenburg, P Jackers, T S Papas, M Ogawa, D K Watson  Hemorrhage, Impaired Hematopoiesis, and Lethality in Mouse Embryos Carrying a Targeted Disruption of the Fli1 Transcription Factor  Mol Cell Biol  2000  20 (15), 5643-52  PubMed ID: 10891501   DOI: 10.1128/mcb.20.15.5643-5652.2000
8887  F Tajima, T Sato, J H Laver, M Ogawa  CD34 Expression by Murine Hematopoietic Stem Cells Mobilized by Granulocyte Colony-Stimulating Factor  Blood  2000  96 (5), 1989-93  PubMed ID: 10961905  
8870  F Hirayama, Y Aiba, K Ikebuchi, S Sekiguchi, M Ogawa  Differentiation in Culture of Murine Primitive Lymphohematopoietic Progenitors Toward T-cell Lineage  Blood  1999  93 (12), 4187-95  PubMed ID: 10361116  
8885  T Sato, J H Laver, M Ogawa  Reversible Expression of CD34 by Murine Hematopoietic Stem Cells  Blood  1999  94 (8), 2548-54  PubMed ID: 10515856  
8872  T Matsunaga, T Kato, H Miyazaki, M Ogawa  Thrombopoietin Promotes the Survival of Murine Hematopoietic Long-Term Reconstituting Cells: Comparison With the Effects of FLT3/FLK-2 Ligand and interleukin-6  Blood  1998  92 (2), 452-61  PubMed ID: 9657744  
8873  T Matsunaga, F Hirayama, Y Yonemura, R Murray, M Ogawa  Negative Regulation by interleukin-3 (IL-3) of Mouse Early B-cell Progenitors and Stem Cells in Culture: Transduction of the Negative Signals by Betac and betaIL-3 Proteins of IL-3 Receptor and Absence of Negative Regulation by Granulocyte-Macrophage Colony-Stimulating Factor  Blood  1998  92 (3), 901-7  PubMed ID: 9680358  
8884  I L Mårtensson, F Melchers, T H Winkler  A Transgenic Marker for Mouse B Lymphoid Precursors  J Exp Med  1997  185 (4), 653-61  PubMed ID: 9034144   DOI: 10.1084/jem.185.4.653
8717  Y Yamamoto, R Yasumizu, Y Amou, N Watanabe, N Nishio, J Toki, S Fukuhara, S Ikehara  Characterization of Peripheral Blood Stem Cells in Mice  Blood  1996  88 (2), 445-54  PubMed ID: 8695791  
8869  D Orlic, L J Girard, C T Jordan, S M Anderson, A P Cline, D M Bodine  The Level of mRNA Encoding the Amphotropic Retrovirus Receptor in Mouse and Human Hematopoietic Stem Cells Is Low and Correlates With the Efficiency of Retrovirus Transduction  Proc Natl Acad Sci U S A  1996  93 (20), 11097-102  PubMed ID: 8855315   DOI: 10.1073/pnas.93.20.11097
8871  N Nishio, H Hisha, H Ogata, M Inaba, Y Yamamoto, Y Amoh, R Yasumizu, K Hanada, H Hamada, S Ikehara  Changes in Markers, Receptors and Adhesion Molecules Expressed on Murine Hemopoietic Stem Cells After a Single Injection of 5-fluorouracil  Stem Cells  1996  14 (5), 584-91  PubMed ID: 8888499   DOI: 10.1002/stem.140584
8868  D Löffert , S Schaal, A Ehlich, R R Hardy, Y R Zou, W Müller, K Rajewsky  Early B-cell Development in the Mouse: Insights From Mutations Introduced by Gene Targeting  Immunol Rev  1994  137, 135-53  PubMed ID: 8034332   DOI: 10.1111/j.1600-065x.1994.tb00662.x
8876  D M Bodine, N E Seidel, M S Gale, A W Nienhuis, D Orlic  Efficient Retrovirus Transduction of Mouse Pluripotent Hematopoietic Stem Cells Mobilized Into the Peripheral Blood by Treatment With Granulocyte Colony-Stimulating Factor and Stem Cell Factor  Blood  1994  84 (5), 1482-91  PubMed ID: 7520774  
3645  Ogawa M, Matsuzaki Y, Nishikawa S, Hayashi S, Kunisada T, Sudo T, Kina T, Nakauchi H, Nishikawa S.  Expression and function of c-kit in hemopoietic progenitor cells.  J Exp Med  1991  174(1):63-71  PubMed ID: 1711568   DOI: 10.1084/jem.174.1.63

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