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Cell No. : Cell Name
RCB4477 : ACK2  update : 2021/12/21
CommentHybridoma cell line producing anti-mouse c-kit (CD117) antibody.
Comment from the depositor
Terms and conditions1) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purpose only. (Commercial use is prohibited by the DEPOSITOR.) 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (EMBO J 1991;10:2111-2118) is necessary.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishikawa, Shin-ichi
Originator Nishikawa, Satomi
Year of deposit 2012
Another name anti mouse c-kit
Animal mouse x rat < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_KB02
hybridoma Fusion_cell_1 name spleen cells
Fusion_cell_1 strain rat, Wistar
Fusion_cell_2 name X63-Ag8/653
Fusion_cell_2 strain mouse
Fusion method PEG #4000, HAT selection
Antibody name ACK2
Subclass rat IgG2b
Immunogen Murine IL-3 dependent mast cells
Antigen_name CD117(c-kit)
Antigen_m.w. 145kDa
Antigen_character transmembrane tyrosin-kinase receptor
Antigen_positive various cells
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10-15 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass rat IgG2b
Images
deposit info
lot info
Reference information Reference 67
User's Publication 0


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Reference
8800  Fu-Li Xiang, Yin Liu 1, Xiangru Lu 1, Douglas L Jones 1, Qingping Feng  Cardiac-specific Overexpression of Human Stem Cell Factor Promotes Epicardial Activation and Arteriogenesis After Myocardial Infarction  Circ Heart Fail  2014  7 (5), 831-42  PubMed ID: 25107671
8832  Ikuko Torii, Shigeki Shimizu, Takashi Daimon, Yoshiyasu Shinohara, Tomoo Kudo, Ayuko Sato, Tohru Tsujimura  Exposure to High Doses of Lipopolysaccharide During Ovalbumin Sensitization Prevents the Development of Allergic Th2 Responses to a Dietary Antigen  J Toxicol Pathol  2014  27 (3-4), 205-15  PubMed ID: 25378805
8821  Tsutomu Motohashi, Katsumasa Yamanaka, Kairi Chiba, Hitomi Aoki, Takahiro Kunisada  Unexpected Multipotency of Melanoblasts Isolated From Murine Skin  Stem Cells  2009  27 (4), 888-97  PubMed ID: 19350691
8852  Tsutomu Motohashi, Hitomi Aoki, Kairi Chiba, Naoko Yoshimura, Takahiro Kunisada  Multipotent Cell Fate of Neural Crest-Like Cells Derived From Embryonic Stem Cells  Stem Cells  2007  25 (2), 402-10  PubMed ID: 17038669
8826  Hongfang Wang, L Jeanne Pierce, Gerald J Spangrude  Distinct Roles of IL-7 and Stem Cell Factor in the OP9-DL1 T-cell Differentiation Culture System  Exp Hematol  2006  34 (12), 1730-40  PubMed ID: 17157170
8789  Rika Okamoto, Masaya Ueno, Yoshihiro Yamada, Naoko Takahashi, Hideto Sano, Toshio Suda, Nobuyuki Takakura  Hematopoietic Cells Regulate the Angiogenic Switch During Tumorigenesis  Blood  2005  105 (7), 2757-63  PubMed ID: 15572584
8704  So-ichiro Fukada, Saito Higuchi, Masashi Segawa, Ken-ichi Koda, Yukiko Yamamoto, Kazutake Tsujikawa, Yasuhiro Kohama, Akiyoshi Uezumi, Michihiro Imamura, Yuko Miyagoe-Suzuki, Shin'ichi Takeda, Hiroshi Yamamoto  Purification and Cell-Surface Marker Characterization of Quiescent Satellite Cells From Murine Skeletal Muscle by a Novel Monoclonal Antibody  Exp Cell Res  2004  296 (2), 245-55  PubMed ID: 15149854
8767  Joanne L Pennock, Richard K Grencis  In Vivo Exit of c-kit+/CD49d(hi)/beta7+ Mucosal Mast Cell Precursors From the Bone Marrow Following Infection With the Intestinal Nematode Trichinella Spiralis  Blood  2004  103 (7), 2655-60  PubMed ID: 14604954
8780  Naoki Sakane, Yoko Asano, Tomoko Kawamura, Tomohiro Takatani, Yasuhiro Kohama, Kazutake Tsujikawa, Hiroshi Yamamoto  Aminopeptidase N/CD13 Regulates the Fetal Liver Microenvironment of Hematopoiesis  Biol Pharm Bull   2004  27 (12), 2014-20  PubMed ID: 15577223
8728  Wilson Wong, J Stephen Billing, Sharon A Stranford, Karen Hyde, Jeremy Fry, Peter J Morris, Kathryn J Wood  Retroviral Transfer of Donor MHC Class I or MHC Class II Genes Into Recipient Bone Marrow Cells Can Induce Operational Tolerance to Alloantigens in Vivo  Hum Gene Ther  2003  14 (6), 577-90  PubMed ID: 12718767
8621  Masanori Nakahara, Koji Isozaki, Jean-Marie Vanderwinden, Yasuhisa Shinomura, Yukihiko Kitamura, Seiichi Hirota, Yuji Matsuzawa  Dose-dependent and Time-Limited Proliferation of Cultured Murine Interstitial Cells of Cajal in Response to Stem Cell Factor  Life Sci   2002  70 (20), 2367-76  PubMed ID: 12150201
8796  Keigo Nishida, Lin Wang, Eiichi Morii, Sung Joo Park, Masahiro Narimatsu, Shousaku Itoh, Satoru Yamasaki, Masahiro Fujishima, Katsuhiko Ishihara, Masahiko Hibi, Yukihiko Kitamura, Toshio Hirano  Requirement of Gab2 for Mast Cell Development and KitL/c-Kit Signaling  Blood  2002  99 (5), 1866-9  PubMed ID: 11861309
8827  Tadashi Okubo, Nobuaki Yanai, Shuntaro Ikawa, Masuo Obinata  Reversible Switching of Expression of C-Kit and Pax-5 in Immature Hematopoietic Progenitor Cells by Stromal Cells  Exp Hematol  2002  30 (10), 1193-201  PubMed ID: 12384151
8797  T Uehara, M Bléry, D W Kang, C C Chen, L H Ho, G L Gartland, F T Liu, E Vivier, M D Cooper, H Kubagawa  Inhibition of IgE-mediated Mast Cell Activation by the Paired Ig-like Receptor PIR-B  Clin Invest  2001  108 (7), 1041-50  PubMed ID: 11581305
8830  T Kouro, K Nagata, S Takaki, S Nisitani, M Hirano, M I Wahl, O N Witte, H Karasuyama, K Takatsu  Bruton's Tyrosine Kinase Is Required for Signaling the CD79b-mediated pro-B to pre-B Cell Transition  Int Immunol  2001  13 (4), 485-93  PubMed ID: 11282988
8858  J Dastych, J Wyczółkowska, D D Metcalfe  Characterization of Alpha 5-integrin-dependent Mast Cell Adhesion Following Fc Epsilon RI Aggregation  Int Arch Allergy Immunol  2001  125 (2), 152-9  PubMed ID: 11435732
8829  H C Hsu, H Ema, M Osawa, Y Nakamura, T Suda, H Nakauchi  Hematopoietic Stem Cells Express Tie-2 Receptor in the Murine Fetal Liver  Blood   2000  96 (12), 3757-62  PubMed ID: 11090057
8831  F Ma, F Yang, A Kaneko, A Manabe, R Tanaka, S Asano, T Nakahata, K Tsuji  Cytokine Requirement for the Development of T-lymphoid Lineage Potential in Clonal Lymphohaematopoietic Progenitors in Vitro  Br J Haematol  2000  111 (4), 1170-9  PubMed ID: 11167758
8859  H Ema 1, H Takano, K Sudo, H Nakauchi  In Vitro Self-Renewal Division of Hematopoietic Stem Cells  J Exp Med  2000  192 (9), 1281-8  PubMed ID: 11067877
8861  K Sudo 1, H Ema, Y Morita, H Nakauchi  Age-associated Characteristics of Murine Hematopoietic Stem Cells  J Exp Med  2000  192 (9), 1273-80  PubMed ID: 11067876
8701  K Kitajima, M Kojima, K Nakajima, S Kondo, T Hara, A Miyajima, T Takeuchi  Definitive but Not Primitive Hematopoiesis Is Impaired in Jumonji Mutant Mice  Blood  1999  93 (1), 87-95  PubMed ID: 9864150
8745  F C Yang , K Tsuji, A Oda, Y Ebihara, M J Xu, A Kaneko, S Hanada, T Mitsui, A Kikuchi, A Manabe, S Watanabe, Y Ikeda, T Nakahata  Differential Effects of Human Granulocyte Colony-Stimulating Factor (hG-CSF) and Thrombopoietin on Megakaryopoiesis and Platelet Function in hG-CSF Receptor-Transgenic Mice  Blood  1999  94 (3), 950-8  PubMed ID: 10419886
8773  I Hamaguchi, X L Huang, N Takakura, J Tada, Y Yamaguchi, H Kodama, T Suda  In Vitro Hematopoietic and Endothelial Cell Development From Cells Expressing TEK Receptor in Murine Aorta-Gonad-Mesonephros Region  Blood  1999  93 (5), 1549-56  PubMed ID: 10029583
8774  F Arai, T Miyamoto, O Ohneda, T Inada, T Sudo, K Brasel, T Miyata, D M Anderson, T Suda  Commitment and Differentiation of Osteoclast Precursor Cells by the Sequential Expression of c-Fms and Receptor Activator of Nuclear Factor kappaB (RANK) Receptors  J Exp Med  1999  190 (12), 1741-54  PubMed ID: 10601350
8794  X L Huang, N Takakura, T Suda  In Vitro Effects of Angiopoietins and VEGF on Hematopoietic and Endothelial Cells  Biochem Biophys Res Commun  1999  264 (1), 133-8  PubMed ID: 10527853
8811  V Revol, I Tsuboi, F Flamant, J Samarut, J P Blanchet, G Mouchiroud  A Novel Growth-Factor-Dependent Myeloid Cell Line Derived From Mouse Bone Marrow Cells Contains Progenitors Endowed With High Proliferative Potential  Exp Hematol  1999  27 (1), 88-98  PubMed ID: 9923447
8898  J Tong, H Kishi, T Matsuda, A Muraguchi  A Bone Marrow-Derived Stroma Cell Line, ST2, Can Support the Differentiation of Fetal Thymocytes From the CD4+ CD8+ Double Negative to the CD4+ CD8+ Double Positive Differentiation Stage in Vitro  Immunology  1999  97 (4), 672-8  PubMed ID: 10457222
8682  M Nakahara, K Isozaki, S Hirota, J Miyagawa, N Hase-Sawada, M Taniguchi, T Nishida, S Kanayama, Y Kitamura, Y Shinomura, Y Matsuzawa  A Novel Gain-Of-Function Mutation of C-Kit Gene in Gastrointestinal Stromal Tumors  Gastroenterology  1998  115 (5), 1090-5  PubMed ID: 9797363
8752  Y Tajima, E J Huang, K Vosseller, M Ono, M A Moore, P Besmer  Role of Dimerization of the Membrane-Associated Growth Factor Kit Ligand in Juxtacrine Signaling: The Sl17H Mutation Affects Dimerization and Stability-Phenotypes in Hematopoiesis  J Exp Med  1998  187 (9), 1451-61  PubMed ID: 9565637
8753  K Akashi, M Kondo, I L Weissman  Two Distinct Pathways of Positive Selection for Thymocytes  Proc Natl Acad Sci U S A  1998  95 (5), 2486-91  PubMed ID: 9482912
8702  H Faulkner, N Humphreys, J C Renauld, J Van Snick, R Grencis  Interleukin-9 Is Involved in Host Protective Immunity to Intestinal Nematode Infection  Eur J Immunol  1997  27 (10), 2536-40  PubMed ID: 9368607
8729  M A Marcos, S Morales-Alcelay, I E Godin, F Dieterlen-Lievre, S G Copin, M L Gaspar  Antigenic Phenotype and Gene Expression Pattern of Lymphohemopoietic Progenitors During Early Mouse Ontogeny  J Immunol  1997  158 (6), 2627-37  PubMed ID: 9058795
8790  A Iguchi, R Okuyama, M Koguma, M Obinata, N Yanai  Selective Stimulation of Granulopoiesis in Vitro by Established Bone Marrow Stromal Cells  Cell Struct Funct  1997  22 (3), 357-64  PubMed ID: 9248999
8819  Y Umemoto, K Tsuji, F C Yang, Y Ebihara, A Kaneko, S Furukawa, T Nakahata  Leptin Stimulates the Proliferation of Murine Myelocytic and Primitive Hematopoietic Progenitor Cells  Blood  1997  90 (9), 3438-43  PubMed ID: 9345027
8600  V C Broudy, N L Lin, G V Priestley, K Nocka, N S Wolf  Interaction of Stem Cell Factor and Its Receptor C-Kit Mediates Lodgment and Acute Expansion of Hematopoietic Cells in the Murine Spleen  Blood  1996  88 (1), 75-81  PubMed ID: 8704204
8617  R S Ismail 1, Y Okawara, J N Fryer, B C Vanderhyden  Hormonal Regulation of the Ligand for C-Kit in the Rat Ovary and Its Effects on Spontaneous Oocyte Meiotic Maturation  Mol Reprod Dev  1996  43 (4), 458-69  PubMed ID: 9052937
8653  L E Donaldson, E Schmitt, J F Huntley, G F Newlands, R K Grencis  A Critical Role for Stem Cell Factor and C-Kit in Host Protective Immunity to an Intestinal Helminth  Int Immunol  1996  8 (4), 559-67  PubMed ID: 8671643
8703  H Nishimura, Y Agata, A Kawasaki, M Sato, S Imamura, N Minato, H Yagita, T Nakano, T Honjo  Developmentally Regulated Expression of the PD-1 Protein on the Surface of Double-Negative (CD4-CD8-) Thymocytes  Int Immunol  1996  8 (5), 773-80  PubMed ID: 8671666
8716  Y Kanamori, K Ishimaru, M Nanno, K Maki, K Ikuta, H Nariuchi, H Ishikawa  Identification of Novel Lymphoid Tissues in Murine Intestinal Mucosa Where Clusters of C-Kit+ IL-7R+ Thy1+ Lympho-Hemopoietic Progenitors Develop  J Exp Med  1996  184 (4), 1449-59  PubMed ID: 8879216
8717  Y Yamamoto, R Yasumizu, Y Amou, N Watanabe, N Nishio, J Toki, S Fukuhara, S Ikehara  Characterization of Peripheral Blood Stem Cells in Mice  Blood  1996  88 (2), 445-54  PubMed ID: 8695791
8718  T Tsujimura, E Morii, M Nozaki, K Hashimoto, Y Moriyama, K Takebayashi, T Kondo, Y Kanakura, Y Kitamura  Involvement of Transcription Factor Encoded by the Mi Locus in the Expression of C-Kit Receptor Tyrosine Kinase in Cultured Mast Cells of Mice  Blood  1996  88 (4), 1225-33  PubMed ID: 8695840
8750  D Guy-Grand, B Cuénod-Jabri, M Malassis-Seris, F Selz, P Vassalli  Complexity of the Mouse Gut T Cell Immune System: Identification of Two Distinct Natural Killer T Cell Intraepithelial Lineages  Eur J Immunol  1996  26 (9), 2248-56  PubMed ID: 8814274
8758  H Ku, Y Yonemura, K Kaushansky, M Ogawa  Thrombopoietin, the Ligand for the Mpl Receptor, Synergizes With Steel Factor and Other Early Acting Cytokines in Supporting Proliferation of Primitive Hematopoietic Progenitors of Mice  Blood  1996  87 (11), 4544-51  PubMed ID: 8639822
8760  E I Deryugina, B I Ratnikov, M A Bourdon, G L Gilmore, R K Shadduck, C E Müller-Sieburg  Identification of a Growth Factor for Primary Murine Stroma as Macrophage Colony-Stimulating Factor  Blood  1996  86 (7), 2568-78  PubMed ID: 7670102
8950  H Kitayama, T Tsujimura, I Matsumura, K Oritani, H Ikeda, J Ishikawa, M Okabe, M Suzuki, K Yamamura, Y Matsuzawa, Y Kitamura, Y Kanakura  Neoplastic Transformation of Normal Hematopoietic Cells by Constitutively Activating Mutations of C-Kit Receptor Tyrosine Kinase  Blood  1996  88 (3), 995-1004  PubMed ID: 8704259
8751  H Kitayama, Y Kanakura, T Furitsu, T Tsujimura, K Oritani, H Ikeda, H Sugahara, H Mitsui, Y Kanayama, Y Kitamura  Constitutively Activating Mutations of C-Kit Receptor Tyrosine Kinase Confer Factor-Independent Growth and Tumorigenicity of Factor-Dependent Hematopoietic Cell Lines  Blood  1995  85 (3), 790-8  PubMed ID: 7530509
8799  F W Jacobsen, C M Dubois, L S Rusten, O P Veiby, S E Jacobsen  Inhibition of Stem Cell Factor-Induced Proliferation of Primitive Murine Hematopoietic Progenitor Cells Signaled Through the 75-kilodalton Tumor Necrosis Factor Receptor. Regulation of C-Kit and p53 Expression  J Immunol  1995  154 (8), 3732-41  PubMed ID: 7535812
8825  R Okuyama, M Koguma, N Yanai, M Obinata  Bone Marrow Stromal Cells Induce Myeloid and Lymphoid Development of the Sorted Hematopoietic Stem Cells in Vitro  Blood  1995  86 (7), 2590-7  PubMed ID: 7545461
8837  H Kurata, T Arai, T Yokota, K Arai  Differential Expression of Granulocyte-Macrophage Colony-Stimulating Factor and IL-3 Receptor Subunits on Human CD34+ Cells and Leukemic Cell Lines  J Allergy Clin Immunol  1995  96 (6 Pt 2), 1083-99  PubMed ID: 8543766
8847  T Moore, M Bennett, V Kumar  Transplantable NK Cell Progenitors in Murine Bone Marrow  J Immunol  1995  154 (4), 1653-63  PubMed ID: 7530741
8736  K Isozaki, T Tsujimura, S Nomura, E Morii, U Koshimizu, Y Nishimune, Y Kitamura  Cell Type-Specific Deficiency of C-Kit Gene Expression in Mutant Mice of Mi/Mi Genotype  Am J Pathol  1994  145 (4), 827-36  PubMed ID: 7524330
8738  M Antica, L Wu, K Shortman, R Scollay  Thymic Stem Cells in Mouse Bone Marrow  Blood  1994  84 (1), 111-7  PubMed ID: 8018910
8775  D I Godfrey, J Kennedy, P Mombaerts, S Tonegawa, A Zlotnik  Onset of TCR-beta Gene Rearrangement and Role of TCR-beta Expression During CD3-CD4-CD8- Thymocyte Differentiation  J Immunol  1994  152 (10), 4783-92  PubMed ID: 7513723
8817  T A Moore, M Vargas-Cortes, R M Welsh, M Bennett, V Kumar  Expression of CZ-1: A CD45RB Epitope on Progenitors of Natural Killer and Other Haematopoietic Cells  Scand J Immunol  1994  39 (3), 257-66  PubMed ID: 7510413
8822  C M Dubois, F W Ruscetti, J Stankova, J R Keller  Transforming Growth Factor-Beta Regulates C-Kit Message Stability and Cell-Surface Protein Expression in Hematopoietic Progenitors  Blood  1994  83 (11), 3138-45  PubMed ID: 7514900
8823  L Cheng, D P Gearing, L S White, D L Compton, K Schooley, P J Donovan  Role of Leukemia Inhibitory Factor and Its Receptor in Mouse Primordial Germ Cell Growth  Development  1994  120 (11), 3145-53  PubMed ID: 7720558
8357  Hisahiro Yoshida Shin‐Ichi Nishikawa Hitoshi Okamura Teruyo Sakakura Moriaki Kusakabe  The Role of c‐kit Proto‐oncogene during Melanocyte Development in Mouse. In vivo Approach by the In utero Microinjection of Anti‐c‐kit Antibody  Develop. Growth & Differ  1993  35(2):209-220 
8611  T Hirata, E Morii, M Morimoto, T Kasugai, T Tsujimura, S Hirota, Y Kanakura, S Nomura, Y Kitamura  Stem Cell Factor Induces Outgrowth of C-Kit-Positive Neurites and Supports the Survival of C-Kit-Positive Neurons in Dorsal Root Ganglia of Mouse Embryos  Development  1993  119 (1), 49-56  PubMed ID: 7506140
8768  R P Bourette, G Mouchiroud, R Ouazana, F Morlé, J Godet, J P Blanchet  Expression of Human Colony-Stimulating factor-1 (CSF-1) Receptor in Murine Pluripotent Hematopoietic NFS-60 Cells Induces Long-Term Proliferation in Response to CSF-1 Without Loss of Erythroid Differentiation Potential  Blood  1993  81 (10), 2511-20  PubMed ID: 7683918
8804  D I Godfrey, J Kennedy, T Suda, A Zlotnik  A Developmental Pathway Involving Four Phenotypically and Functionally Distinct Subsets of CD3-CD4-CD8- Triple-Negative Adult Mouse Thymocytes Defined by CD44 and CD25 Expression  J Immunol  1993  150 (10), 4244-52  PubMed ID: 8387091
8833  M Antica, L Wu, K Shortman, R Scollay  Intrathymic Lymphoid Precursor Cells During Fetal Thymus Development  J Immunol  1993  151 (11), 5887-95  PubMed ID: 8245436
8676  R L Phillips, A J Reinhart, G Van Zant  Genetic Control of Murine Hematopoietic Stem Cell Pool Sizes and Cycling Kinetics  Proc Natl Acad Sci U S A   1992  89 (23), 11607-11   PubMed ID: 1280831
8699  D I Godfrey, A Zlotnik, T Suda  Phenotypic and Functional Characterization of C-Kit Expression During Intrathymic T Cell Development  J Immunol   1992  149 (7), 2281-5  PubMed ID: 1382094
8805  R Palacios, J Samaridis  Fetal Liver pro-B and pre-B Lymphocyte Clones: Expression of Lymphoid-Specific Genes, Surface Markers, Growth Requirements, Colonization of the Bone Marrow, and Generation of B Lymphocytes in Vivo and in Vitro  Mol Cell Biol  1992  12 (2), 518-30  PubMed ID: 1346335
5507  Nishikawa S, Kusakabe M, Yoshinaga K, Ogawa M, Hayashi S, Kunisada T, Era T, Sakakura T, Nishikawa S.  In utero manipulation of coat color formation by a monoclonal anti-c-kit antibody: two distinct waves of c-kit-dependency during melanocyte development.  EMBO J  1991  10(8):2111-8  PubMed ID: 1712289
8378  S Okada, H Nakauchi, K Nagayoshi, S Nishikawa, S Nishikawa, Y Miura, T Suda  Enrichment and Characterization of Murine Hematopoietic Stem Cells That Express C-Kit Molecule  Blood  1991  78 (7), 1706-12  PubMed ID: 1717068
8599  A Rolink , M Streb, S Nishikawa, F Melchers  The C-Kit-Encoded Tyrosine Kinase Regulates the Proliferation of Early pre-B Cells  Eur J Immunol  1991  21 (10), 2609-12  PubMed ID: 1717287

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